Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,616,162	C→A	A23A (GCC→GCA)	bepA →	beta‑barrel assembly‑enhancing protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,616,162	0	C	A	100.0%	245.5 / NA	72	A23A (GCC→GCA)	bepA	beta‑barrel assembly‑enhancing protease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (42/30);  total (42/30)

CGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCCCCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTACGCGGCAGCGCGCCGTTAATTAAT  >  NC_000913/2616025‑2616297                                                                                                                                          |                                                                                                                                        cggcgCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGcacc                                                                                                                                       <  1:638479/140‑1 (MQ=255)        gTGGGAACGATATTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGttt                                                                                                                                >  1:794325/1‑140 (MQ=255)          gggAACGATATTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGc                                                                                                                              >  2:855753/1‑140 (MQ=255)             aaCGATATTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGa                                                                                                                           <  2:1264813/140‑1 (MQ=255)              aCGATATTTCACAGTATAGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGAc                                                                                                                          >  2:1258417/1‑140 (MQ=255)                    tttCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCa                                                                                                                    <  1:1154316/140‑1 (MQ=255)                     ttCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAgcgc                                                                                                                     >  1:466755/1‑138 (MQ=255)                     ttCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAgcgc                                                                                                                     <  2:466755/138‑1 (MQ=255)                       cacaGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGac                                                                                                                 >  1:1091469/1‑140 (MQ=255)                            tATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCtt                                                                                                            >  2:1018258/1‑140 (MQ=255)                            tATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCtc                                                                                                            >  2:1104208/1‑139 (MQ=255)                                 gTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCgg                                                                                                       >  1:1277727/1‑140 (MQ=255)                                 gTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCgg                                                                                                       <  2:1186592/140‑1 (MQ=255)                                      aTGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATg                                                                                                  >  1:99443/1‑140 (MQ=255)                                            aTCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAAcc                                                                                            >  1:1350625/1‑140 (MQ=255)                                            aTCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAAcc                                                                                            >  2:770135/1‑140 (MQ=255)                                                     aaTACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGa                                                                                   >  1:1173189/1‑140 (MQ=255)                                                       tACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAg                                                                                 >  1:255969/1‑140 (MQ=255)                                                        aCAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGc                                                                                <  1:1018258/140‑1 (MQ=255)                                                         cAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCa                                                                               >  1:350985/1‑140 (MQ=255)                                                         cAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCa                                                                               >  1:999229/1‑140 (MQ=255)                                                           ggATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACg                                                                             <  2:921501/140‑1 (MQ=255)                                                            gATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGc                                                                            >  1:68442/1‑140 (MQ=255)                                                             aTAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCt                                                                           <  2:1091469/140‑1 (MQ=255)                                                                gagGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTc                                                                        <  2:255969/140‑1 (MQ=255)                                                                  ggTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCa                                                                      <  2:916260/140‑1 (MQ=255)                                                                   gTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCATCCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCAt                                                                     <  2:654025/140‑1 (MQ=255)                                                                   gTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCAt                                                                     <  2:1196775/140‑1 (MQ=255)                                                                   gTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCAt                                                                     <  1:1258417/140‑1 (MQ=255)                                                                   gTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCAt                                                                     <  2:1277727/140‑1 (MQ=255)                                                                      aTGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTgg                                                                  >  1:877714/1‑140 (MQ=255)                                                                      aTGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTgg                                                                  >  2:1112058/1‑140 (MQ=255)                                                                      aTGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTgg                                                                  >  1:249171/1‑140 (MQ=255)                                                                      aTGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTgg                                                                  >  2:153256/1‑140 (MQ=255)                                                                      aTGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTgg                                                                  <  1:855753/140‑1 (MQ=255)                                                                      aTGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTgg                                                                  >  2:168755/1‑140 (MQ=255)                                                                        gTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCa                                                                      <  2:660367/134‑1 (MQ=255)                                                                        gTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCa                                                                      >  1:660367/1‑134 (MQ=255)                                                                            aGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGa                                                            >  1:31153/1‑140 (MQ=255)                                                                             ggCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTgg                                                                  <  2:4265/133‑1 (MQ=255)                                                                             ggCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTgg                                                                  >  1:4265/1‑133 (MQ=255)                                                                                  ttGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCa                                                      >  1:931831/1‑140 (MQ=255)                                                                                  ttGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCa                                                      >  2:180993/1‑140 (MQ=255)                                                                                   tGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAg                                                     >  2:868975/1‑140 (MQ=255)                                                                                   tGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAg                                                     >  1:1053453/1‑140 (MQ=255)                                                                                   tGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAg                                                     >  1:704504/1‑140 (MQ=255)                                                                                    gAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGa                                                    <  2:68442/140‑1 (MQ=255)                                                                                      aaaaaaaCCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATg                                                  >  2:854302/1‑140 (MQ=255)                                                                                        aaaaaCCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATggg                                                <  1:770135/140‑1 (MQ=255)                                                                                        aaaaaCCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATggg                                                >  2:44779/1‑140 (MQ=255)                                                                                        aaaaaCCAGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATggg                                                <  2:1082154/140‑1 (MQ=255)                                                                                         aaaaCCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGc                                               <  1:298874/140‑1 (MQ=255)                                                                                          aaaCCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCg                                              <  1:1112058/140‑1 (MQ=255)                                                                                           aaCCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGa                                             <  2:933191/140‑1 (MQ=255)                                                                                               tGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATggg                                                >  1:82496/1‑133 (MQ=255)                                                                                               tGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATggg                                                <  2:82496/133‑1 (MQ=255)                                                                                               tGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACtat                                         >  1:862548/1‑140 (MQ=255)                                                                                                 gTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATACGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACtatta                                       >  2:692492/1‑140 (MQ=255)                                                                                                     cAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTc                                   >  1:577156/1‑140 (MQ=255)                                                                                                            cATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGa                                             >  1:1230355/1‑123 (MQ=255)                                                                                                            cATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGa                                             <  2:1230355/123‑1 (MQ=255)                                                                                                               tgctgcTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGcc                               >  2:519565/1‑134 (MQ=255)                                                                                                               tgctgcTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGcc                               <  1:519565/134‑1 (MQ=255)                                                                                                               tgctgcTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTAc                         >  2:630381/1‑140 (MQ=255)                                                                                                                  tgctATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTAcgcg                      >  2:1163904/1‑140 (MQ=255)                                                                                                                   gctATGTCCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTACGCgg                     >  1:803993/1‑140 (MQ=255)                                                                                                                       tGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTACGCGGCAgc                 >  2:1358060/1‑140 (MQ=255)                                                                                                                         aCCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTACGCGGCAgcgc               >  2:218856/1‑140 (MQ=255)                                                                                                                          ccATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTACGCGGCAgcgcg              <  2:577156/140‑1 (MQ=255)                                                                                                                          ccATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTACGCGGCAgcgcg              <  1:168755/140‑1 (MQ=255)                                                                                                                             ttGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTACGCGGCAGCGCGCCg           <  2:593000/140‑1 (MQ=255)                                                                                                                                      tAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTACGCGGCAGCGCGCCGTtaattaat  <  1:1358060/140‑1 (MQ=255)                                                                                                                                      tAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATACGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTACGCGGCAGCGCGCCGTtaattaat  <  1:692492/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCCCCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTACGCGGCAGCGCGCCGTTAATTAAT  >  NC_000913/2616025‑2616297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑