Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,886,861	T→C	Y120C (TAT→TGT)	cas3 ←	CRISPR‑associated endonuclease/helicase Cas3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,886,861	0	T	C	100.0%	318.8 / NA	81	Y120C (TAT→TGT)	cas3	CRISPR‑associated endonuclease/helicase Cas3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/46);  total (35/46)

TGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCAAACTTTCCA  >  NC_000913/2886726‑2886992                                                                                                                                        |                                                                                                                                    tGTAATATAAAACCATGATGTTCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAg                                                                                                                                 >  2:1331225/1‑140 (MQ=255) tGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAg                                                                                                                                 <  1:1262927/140‑1 (MQ=255)   tAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGAc                                                                                                                               <  1:1246797/140‑1 (MQ=255)   tAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGAc                                                                                                                               <  1:1142817/140‑1 (MQ=255)       ataAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGc                                                                                                                           <  1:1179558/140‑1 (MQ=255)        taAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATC‑AAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTg                                                                                                                         <  2:776488/140‑1 (MQ=255)         aaaaCCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTg                                                                                                                         <  1:868509/140‑1 (MQ=255)             ccATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCAcc                                                                                                                     >  2:894557/1‑140 (MQ=255)                  atgTTCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGAt                                                                                                                <  1:1331225/140‑1 (MQ=255)                          tAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTa                                                                                                        <  1:493067/140‑1 (MQ=255)                            aCGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACg                                                                                                      <  2:478405/140‑1 (MQ=255)                             cGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACgg                                                                                                     <  2:310525/140‑1 (MQ=255)                               gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGAtt                                                                                                               >  2:380023/1‑128 (MQ=255)                               gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGAtt                                                                                                               <  1:380023/128‑1 (MQ=255)                               gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAAtt                                                                                                          >  2:1114729/1‑133 (MQ=255)                               gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAAtt                                                                                                          <  1:1114729/133‑1 (MQ=255)                               gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACgg                                                                                                     >  1:1273896/1‑138 (MQ=255)                               gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACgg                                                                                                     <  2:1273896/138‑1 (MQ=255)                               gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGca                                                                                                   >  1:142054/1‑140 (MQ=255)                               gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGca                                                                                                   <  2:784742/140‑1 (MQ=255)                               gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGca                                                                                                   >  1:686905/1‑140 (MQ=255)                                  tctACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACAt                                                                                                >  2:129319/1‑140 (MQ=255)                                    tACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACAttt                                                                                              >  1:248506/1‑140 (MQ=255)                                     aCCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTtg                                                                                             <  1:902447/140‑1 (MQ=255)                                        cATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTgtgt                                                                                          <  2:551609/140‑1 (MQ=255)                                         aTGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGtt                                                                                         >  1:342443/1‑140 (MQ=255)                                             aaaCCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATg                                                                                     <  2:1207302/140‑1 (MQ=255)                                              aaCCAGGACTCATAATGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATgg                                                                                    <  2:139735/140‑1 (MQ=255)                                              aaCCAGGACTCATAAGGATGAGGAGCGGCATCAAAAATACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATgg                                                                                    <  2:644852/140‑1 (MQ=255)                                              aaCCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATgg                                                                                    <  2:342905/140‑1 (MQ=255)                                                ccAGGACTCATAAGGATGAGGAGCGGCCTCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGcac                                                                                                  >  1:306065/1‑124 (MQ=255)                                                ccAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGcac                                                                                                  <  2:306065/124‑1 (MQ=255)                                                   ggACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCAt                                                                               >  1:534581/1‑140 (MQ=255)                                                    gACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCAtt                                                                              >  1:617434/1‑140 (MQ=255)                                                         aTAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTg                                                                         >  1:1246216/1‑140 (MQ=255)                                                          tAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGa                                                                        <  2:342443/140‑1 (MQ=255)                                                           aaGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGAt                                                                       <  2:534581/140‑1 (MQ=255)                                                                 gaggagCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGtt                                                                 >  1:157612/1‑140 (MQ=255)                                                                 gaggagCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGtt                                                                 >  1:189386/1‑140 (MQ=255)                                                                  aggagCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTg                                                                <  1:431591/140‑1 (MQ=255)                                                                  aggagCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTg                                                                <  2:617434/140‑1 (MQ=255)                                                                   ggagCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGc                                                               >  1:313801/1‑140 (MQ=255)                                                                       cGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGa                                                           >  1:1094510/1‑140 (MQ=255)                                                                        ggCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGAt                                                          >  1:995386/1‑140 (MQ=255)                                                                         gCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGc                                                               >  1:1326514/1‑134 (MQ=255)                                                                         gCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGc                                                               >  1:1376729/1‑134 (MQ=255)                                                                         gCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGc                                                               <  2:1326514/134‑1 (MQ=255)                                                                         gCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGc                                                               <  2:1376729/134‑1 (MQ=255)                                                                         gCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGAtt                                                         >  1:670343/1‑140 (MQ=255)                                                                         gCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGAtt                                                         >  1:1090702/1‑140 (MQ=255)                                                                         gCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGAtt                                                         >  1:80069/1‑140 (MQ=255)                                                                         gCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGAtt                                                         >  2:191764/1‑140 (MQ=255)                                                                            tCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTaa                                                      >  1:783576/1‑140 (MQ=255)                                                                            tCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTaa                                                      >  2:489613/1‑140 (MQ=255)                                                                                             aTCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTctg                                     <  2:670343/140‑1 (MQ=255)                                                                                             aTCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTctg                                     <  2:142054/140‑1 (MQ=255)                                                                                              tCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTctgc                                    <  2:995386/140‑1 (MQ=255)                                                                                                  cGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGAt                                <  2:384752/140‑1 (MQ=255)                                                                                                   gagagaTTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGAtt                               <  2:313801/140‑1 (MQ=255)                                                                                                     gagaTTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTta                             <  2:248506/140‑1 (MQ=255)                                                                                                        aTTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTctg                                     <  2:1080762/129‑1 (MQ=255)                                                                                                        aTTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTctg                                     <  2:367988/129‑1 (MQ=255)                                                                                                        aTTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTctg                                     >  1:1080762/1‑129 (MQ=255)                                                                                                        aTTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTctg                                     >  1:367988/1‑129 (MQ=255)                                                                                                         ttGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACttt                                        <  2:1175461/125‑1 (MQ=255)                                                                                                         ttGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACttt                                        >  1:1175461/1‑125 (MQ=255)                                                                                                            ctctGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGa                      >  1:893760/1‑140 (MQ=255)                                                                                                             tctGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGaa                     <  1:489613/140‑1 (MQ=255)                                                                                                               tGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATc                   <  1:1198399/140‑1 (MQ=255)                                                                                                                  aaGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGta                >  2:960342/1‑140 (MQ=255)                                                                                                                    gTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGtata              >  2:1019431/1‑140 (MQ=255)                                                                                                                       aaTCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCa           >  2:307402/1‑140 (MQ=255)                                                                                                                         tCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCaaa         <  1:462168/140‑1 (MQ=255)                                                                                                                         tCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCaaa         <  1:894557/140‑1 (MQ=255)                                                                                                                         tCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCaaa         <  2:424751/140‑1 (MQ=255)                                                                                                                         tCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCaaa         <  1:1019431/140‑1 (MQ=255)                                                                                                                           cTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCAAACt       >  1:1042227/1‑140 (MQ=255)                                                                                                                              gTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCAAACTTTc    <  2:1042227/140‑1 (MQ=255)                                                                                                                              gTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCAAACTTTc    <  2:1094510/140‑1 (MQ=255)                                                                                                                                tAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCAAACTTTCCa  <  2:570116/140‑1 (MQ=255)                                                                                                                                tAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCAAACTTTCCa  <  2:893760/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    TGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCAAACTTTCCA  >  NC_000913/2886726‑2886992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑