Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,835,418	T→C	N171S (AAT→AGT)	yicJ ←	putative xyloside transporter YicJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,835,418	0	T	C	100.0%	359.2 / NA	91	N171S (AAT→AGT)	yicJ	putative xyloside transporter YicJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/41);  total (50/41)

CGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATT  >  NC_000913/3835285‑3835551                                                                                                                                      |                                                                                                                                          cGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCa                                                                                                                                 <  1:219940/140‑1 (MQ=255)  gttgttGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAg                                                                                                                                <  1:41225/140‑1 (MQ=255)   ttgttgTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGt                                                                                                                               >  2:1031221/1‑140 (MQ=255)      ttgtAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCCCGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGc                                                                                                                            <  2:293083/140‑1 (MQ=255)      ttgtAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGc                                                                                                                            <  1:48344/140‑1 (MQ=255)      ttgtAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGc                                                                                                                            <  1:253132/140‑1 (MQ=255)      ttgtAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGc                                                                                                                            <  2:1174250/140‑1 (MQ=255)          aGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGcatca                                                                                                                        >  2:1299721/1‑140 (MQ=255)          aGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGcatca                                                                                                                        >  1:870031/1‑140 (MQ=255)           ggtggtGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGcatcat                                                                                                                       >  1:1104534/1‑140 (MQ=255)              ggtgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAg                                                                                                                    <  2:1041451/140‑1 (MQ=255)               gtgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGa                                                                                                                   <  1:663856/140‑1 (MQ=255)               gtgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGa                                                                                                                   >  2:640327/1‑140 (MQ=255)               gtgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGa                                                                                                                   >  1:805430/1‑140 (MQ=255)                tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGaa                                                                                                                  >  1:546815/1‑140 (MQ=255)                tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGaa                                                                                                                  >  1:339820/1‑140 (MQ=255)                tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGaa                                                                                                                  >  1:870069/1‑140 (MQ=255)                tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGaa                                                                                                                  >  2:115840/1‑140 (MQ=255)                tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGaa                                                                                                                  >  2:1002422/1‑140 (MQ=255)                     cAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTa                                                                                                             >  1:276810/1‑140 (MQ=255)                     cAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTa                                                                                                             <  2:268984/140‑1 (MQ=255)                          cgTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAg                                                                                                        >  1:343352/1‑140 (MQ=255)                            ttCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCa                                                                                                      >  2:792828/1‑140 (MQ=255)                            ttCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGCTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCa                                                                                                      >  1:1334803/1‑140 (MQ=255)                             tCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCAt                                                                                                     <  1:792828/140‑1 (MQ=255)                                       tgAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCcgcg                                                                                           >  2:911749/1‑140 (MQ=255)                                         aagaaACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGt                                                                                         >  1:944878/1‑140 (MQ=255)                                         aagaaACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGt                                                                                         >  1:73069/1‑140 (MQ=255)                                         aagaaACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGt                                                                                         >  2:1069955/1‑140 (MQ=255)                                         aagaaACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGt                                                                                         >  1:100228/1‑140 (MQ=255)                                         aagaaACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGt                                                                                         >  2:22561/1‑140 (MQ=255)                                                aaaaTGCCAGCATCATGAATACCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGc                                                                                  >  1:1328452/1‑140 (MQ=255)                                                      ccAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACaaaa                                                                            >  1:1123141/1‑140 (MQ=255)                                                              atGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGa                                                                    <  1:147218/140‑1 (MQ=255)                                                               tGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGAt                                                                   >  2:183006/1‑140 (MQ=255)                                                                gAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGAtt                                                                  <  1:450301/140‑1 (MQ=255)                                                                   tGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCa                                                               <  1:843957/140‑1 (MQ=255)                                                                       accacGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCga                                                           >  1:1031107/1‑140 (MQ=255)                                                                        ccacGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCgag                                                          <  2:343352/140‑1 (MQ=255)                                                                         cacGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCgaga                                                         >  2:1030364/1‑140 (MQ=255)                                                                             gAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACg                                                     <  1:598461/140‑1 (MQ=255)                                                                              aaaGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGc                                                    >  1:264704/1‑140 (MQ=255)                                                                                aGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATAc                                                      <  1:1082915/140‑1 (MQ=255)                                                                                aGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTg                                                  >  2:1321277/1‑140 (MQ=255)                                                                                 ggACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGa                                                 <  1:22561/140‑1 (MQ=255)                                                                                   aCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGt                                               <  1:1146495/140‑1 (MQ=255)                                                                                   aCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGt                                               <  2:1123141/140‑1 (MQ=255)                                                                                   aCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGt                                               <  2:276810/140‑1 (MQ=255)                                                                                   aCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGt                                               >  2:146675/1‑140 (MQ=255)                                                                                     cgcgATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCg                                             >  2:298245/1‑140 (MQ=255)                                                                                     cgcgATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCg                                             >  2:373453/1‑140 (MQ=255)                                                                                     cgcgATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCg                                             >  2:1073508/1‑140 (MQ=255)                                                                                      gcgATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCgg                                            >  2:982635/1‑140 (MQ=255)                                                                                      gcgATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCgg                                            >  1:847152/1‑140 (MQ=255)                                                                                       cgATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCggg                                           >  1:790885/1‑140 (MQ=255)                                                                                        gATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGt                                          <  1:329611/140‑1 (MQ=255)                                                                                          tACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCa                                        <  2:805430/140‑1 (MQ=255)                                                                                            ccGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCAtt                                      <  2:1104534/140‑1 (MQ=255)                                                                                            ccGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCAtt                                      <  2:73069/140‑1 (MQ=255)                                                                                            ccGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCAtt                                      <  2:944878/140‑1 (MQ=255)                                                                                             cGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTg                                     >  2:238773/1‑140 (MQ=255)                                                                                               cccTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGt                                   >  1:374897/1‑140 (MQ=255)                                                                                               cccTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGt                                   >  1:192756/1‑140 (MQ=255)                                                                                                ccTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCggg                                           <  1:568289/131‑1 (MQ=255)                                                                                                ccTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCggg                                           >  2:568289/1‑131 (MQ=255)                                                                                                 cTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGa                                 <  2:1328452/140‑1 (MQ=255)                                                                                                  tGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGAt                                >  2:626809/1‑140 (MQ=255)                                                                                                       aCCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGt                                   >  1:605719/1‑132 (MQ=255)                                                                                                       aCCGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGt                                   <  2:605719/132‑1 (MQ=255)                                                                                                        ccGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTAcacc                          <  2:603629/140‑1 (MQ=255)                                                                                                         cGAGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTAcacca                         >  1:716527/1‑140 (MQ=255)                                                                                                           aGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACaccacc                       >  2:1245980/1‑140 (MQ=255)                                                                                                           aGTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACaccacc                       >  1:967771/1‑140 (MQ=255)                                                                                                            gTGGTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCAccc                      >  2:620641/1‑140 (MQ=255)                                                                                                              ggTTTATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACACTGAGTCGGGTCATTGGTGATTACACCACCCaa                    >  2:642516/1‑140 (MQ=255)                                                                                                                tttATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATg                  <  2:790885/140‑1 (MQ=255)                                                                                                                tttATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATg                  <  2:716527/140‑1 (MQ=255)                                                                                                                tttATTATCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATg                  <  1:1069955/140‑1 (MQ=255)                                                                                                                  tattatCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATgcg                <  1:1245980/140‑1 (MQ=255)                                                                                                                  tattatCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATgcg                <  2:870031/140‑1 (MQ=255)                                                                                                                  tattatCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATgcg                <  2:100228/140‑1 (MQ=255)                                                                                                                  tattatCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATgcg                <  1:298245/140‑1 (MQ=255)                                                                                                                  tattatCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATgcg                <  1:911749/140‑1 (MQ=255)                                                                                                                      atCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGt            <  1:1073508/140‑1 (MQ=255)                                                                                                                      atCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGt            <  2:870069/140‑1 (MQ=255)                                                                                                                       tCACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTAGGGTCATTGGTGATTACACCACCCAATGCGCAGTa           >  1:451190/1‑140 (MQ=255)                                                                                                                        cACCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTaa          <  1:1031221/140‑1 (MQ=255)                                                                                                                         aCCGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAg         >  2:557194/1‑140 (MQ=255)                                                                                                                          ccGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAgg        >  2:169091/1‑140 (MQ=255)                                                                                                                          ccGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAgg        >  1:1001754/1‑140 (MQ=255)                                                                                                                           cGCCAATTAAACT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAggg       <  1:640327/140‑1 (MQ=255)                                                                                                                                attaaact‑‑‑‑aaCCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATAtt  <  2:857932/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                          CGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATT‑‑‑‑AACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATT  >  NC_000913/3835285‑3835551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑