Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,649,867	T→G	V25G (GTT→GGT)	ydfD →	Qin prophage; lysis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,649,867	0	T	G	100.0%	319.8 / NA	81	V25G (GTT→GGT)	ydfD	Qin prophage; lysis protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (36/45);  total (36/45)

AAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGTTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAGGTCTTTAAAACAGTTCCGTAATAAA  >  NC_000913/1649731‑1650002                                                                                                                                         |                                                                                                                                        aaataaaataTGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTca                                                                                                                                      >  2:584586/1‑140 (MQ=255)      aaataTGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGa                                                                                                                                 <  1:573334/140‑1 (MQ=255)       aataTGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGAc                                                                                                                                >  2:834867/1‑140 (MQ=255)       aataTGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGAc                                                                                                                                >  2:85882/1‑140 (MQ=255)         tatGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTTACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACaa                                                                                                                              >  2:641105/1‑140 (MQ=255)         tatGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACaa                                                                                                                              >  1:698647/1‑140 (MQ=255)         tatGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACaa                                                                                                                              >  1:1294557/1‑140 (MQ=255)            gCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGc                                                                                                                           <  1:830648/140‑1 (MQ=255)             cATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCa                                                                                                                          >  2:1344620/1‑140 (MQ=255)              aTTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAg                                                                                                                         <  2:698647/140‑1 (MQ=255)              aTTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAg                                                                                                                         <  1:565321/140‑1 (MQ=255)              aTTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAg                                                                                                                         <  2:606135/140‑1 (MQ=255)              aTTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAg                                                                                                                         <  2:525498/140‑1 (MQ=255)              aTTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAg                                                                                                                         <  2:524034/140‑1 (MQ=255)               ttGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTca                                                                                                                                      <  2:1384213/126‑1 (MQ=255)               ttGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTca                                                                                                                                      >  1:1384213/1‑126 (MQ=255)                tGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGa                                                                                                                       >  2:1302795/1‑140 (MQ=255)                 gTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAg                                                                                                                      >  1:169269/1‑140 (MQ=255)                  tttCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGa                                                                                                                                 >  2:1277198/1‑128 (MQ=255)                  tttCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGa                                                                                                                                 <  1:1277198/128‑1 (MQ=255)                  tttCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAgt                                                                                                                     >  2:819909/1‑140 (MQ=255)                  tttCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAgt                                                                                                                     >  1:862557/1‑140 (MQ=255)                       aTGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATg                                                                                                                >  1:1272188/1‑140 (MQ=255)                            ggCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAg                                                                                                                         <  1:308957/126‑1 (MQ=255)                            ggCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAg                                                                                                                         >  2:308957/1‑126 (MQ=255)                            ggCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTgc                                                                                                           >  1:975611/1‑140 (MQ=255)                            ggCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTgc                                                                                                           >  1:1270578/1‑140 (MQ=255)                                  tttACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAAc                                                                                                     >  2:244570/1‑140 (MQ=255)                                  tttACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAAc                                                                                                     <  2:171649/140‑1 (MQ=255)                                  tttACGTCTGATGCCAAAAGGATGTGCACAAAGAATTCCGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAAc                                                                                                     >  2:247245/1‑140 (MQ=255)                                    tACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTACAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCg                                                                                                   <  2:1157051/140‑1 (MQ=255)                                     aCGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGa                                                                                                  <  1:658405/140‑1 (MQ=255)                                            aTGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGaaa                                                                                           >  2:1286516/1‑140 (MQ=255)                                            aTGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGaaa                                                                                           >  1:48363/1‑140 (MQ=255)                                            aTGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGaaa                                                                                           >  1:911269/1‑140 (MQ=255)                                               ccAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAAtt                                                                                        <  1:1302795/140‑1 (MQ=255)                                                    aGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCgg                                                                                   <  2:1203916/140‑1 (MQ=255)                                                    aGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCgg                                                                                   <  1:318355/140‑1 (MQ=255)                                                     ggATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGt                                                                                  <  1:834867/140‑1 (MQ=255)                                                     ggATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGt                                                                                  <  1:621504/140‑1 (MQ=255)                                                     ggATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGt                                                                                  <  1:534085/140‑1 (MQ=255)                                                        tgtgCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAAc                                                                               <  1:85882/140‑1 (MQ=255)                                                                 tGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCg                                                                      >  1:1279362/1‑140 (MQ=255)                                                                 tGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCg                                                                      >  2:323672/1‑140 (MQ=255)                                                                       cAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGAt                                                                >  2:847474/1‑140 (MQ=255)                                                                          cATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATaaa                                                             <  1:847474/140‑1 (MQ=255)                                                                           aTTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAg                                                            >  1:725042/1‑140 (MQ=255)                                                                           aTTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAg                                                            <  1:244570/140‑1 (MQ=255)                                                                             ttGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGtt                                                          >  1:114427/1‑140 (MQ=255)                                                                              tgtgCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTa                                                         <  2:48363/140‑1 (MQ=255)                                                                              tgtgCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTa                                                         <  2:1294557/140‑1 (MQ=255)                                                                              tgtgCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTa                                                         <  1:323672/140‑1 (MQ=255)                                                                               gtgCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTAt                                                        >  2:1224768/1‑140 (MQ=255)                                                                                tgCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTAtt                                                       <  2:1272188/140‑1 (MQ=255)                                                                                 gCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTc                                                      <  2:210011/140‑1 (MQ=255)                                                                                   ttGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCAc                                                    <  1:1004450/140‑1 (MQ=255)                                                                                    tGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCAcc                                                   >  2:648164/1‑140 (MQ=255)                                                                                     gTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCa                                                  >  2:1280431/1‑140 (MQ=255)                                                                                       tCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGa                                                                 >  2:1188364/1‑123 (MQ=255)                                                                                       tCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGa                                                                 <  1:1188364/123‑1 (MQ=255)                                                                                       tCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAgg                                                <  2:610486/140‑1 (MQ=255)                                                                                         tGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGat                                              <  2:975611/140‑1 (MQ=255)                                                                                         tGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGat                                              <  1:1099125/140‑1 (MQ=255)                                                                                               tttttCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCAc                                                    >  1:482944/1‑128 (MQ=255)                                                                                               tttttCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCAc                                                    <  2:482944/128‑1 (MQ=255)                                                                                                    cTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAAt                                   <  2:862557/140‑1 (MQ=255)                                                                                                     ttGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATc                                  >  1:839389/1‑140 (MQ=255)                                                                                                          tCCGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGc                             <  2:1270578/140‑1 (MQ=255)                                                                                                            cGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAg                           <  2:1336541/140‑1 (MQ=255)                                                                                                            cGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAg                           <  2:639620/140‑1 (MQ=255)                                                                                                            cGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAg                           <  1:819909/140‑1 (MQ=255)                                                                                                            cGGAGAGCCAGTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAg                           <  2:839389/140‑1 (MQ=255)                                                                                                            cGGAGAGCCAGTTGATATTGCAGTCAATGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAg                           <  2:450365/140‑1 (MQ=255)                                                                                                                      gTTGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAGGTCTTTaaaa                 <  2:911269/140‑1 (MQ=255)                                                                                                                        tGATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAGGTCTTTAAAACa               <  1:648164/140‑1 (MQ=255)                                                                                                                         gATATTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAGGTCTTTAAAACAg              <  1:1344620/140‑1 (MQ=255)                                                                                                                            atTGCAGTCAGTGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAGGTCTTTAAAACAGTTc           >  1:282463/1‑140 (MQ=255)                                                                                                                                 agtcagtGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAGGTCTTTAAAACAGTTCCGtaa      >  2:877762/1‑140 (MQ=255)                                                                                                                                  gtcagtGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAGGTCTTTAAAACAGTTCCGtaat     <  2:46004/140‑1 (MQ=255)                                                                                                                                     agtGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAGGTCTTTAAAACAGTTCCGtaata    >  1:1365947/1‑138 (MQ=255)                                                                                                                                     agtGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAGGTCTTTAAAACAGTTCCGtaata    <  2:1365947/138‑1 (MQ=255)                                                                                                                                     agtGGTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAGGTCTTTAAAACAGTTCCGTaataaa  >  1:1352625/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGTCAGTGTTCACAGGACAATGCAGGAGTGTATGACTGCAGCAACCGAACAGAAAATTCCCGGTAACTGTTACCCGGTCGATAAAGTTATTCACCAGGATAATATCGAAATCCCGGCAGGTCTTTAAAACAGTTCCGTAATAAA  >  NC_000913/1649731‑1650002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑