| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,917,090 | T→G | A678A (GCT→GCG) | barA → | sensory histidine kinase BarA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,917,090 | 0 | T | G | 97.3% | 134.2 / ‑3.9 | 37 | A678A (GCT→GCG) | barA | sensory histidine kinase BarA |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (4/32); total (4/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
GCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA > NC_000913/2916955‑2917226 | gctgAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAAcc > 2:899580/1‑140 (MQ=255)gctgAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAAcc < 2:949610/140‑1 (MQ=255) aaCCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGaaa < 1:115259/140‑1 (MQ=255) tAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAAACCCCCGGACCTGAAAATTAtt > 2:115259/1‑139 (MQ=255) gccTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGgcg < 1:1109933/139‑1 (MQ=255) gagacagcGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGgcg < 1:831847/133‑1 (MQ=255) cTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCa < 1:603914/140‑1 (MQ=255) cacGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTg < 1:745961/140‑1 (MQ=255) cacGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTg > 1:962025/1‑140 (MQ=255) cGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCt < 1:749337/140‑1 (MQ=255) ccTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTgg < 2:582649/140‑1 (MQ=255) gTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTTAAGTCAACCCCTCCTACCCGTAACTTATTGGCGCCTTTGTGGaaa > 2:1109933/1‑139 (MQ=255) gTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAg > 2:113043/1‑140 (MQ=255) ttGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCCCGAACCTGAAACTTATCGGCGCATTGCTGGAAGa > 1:150474/1‑140 (MQ=255) tgcctgccCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGat < 1:295409/140‑1 (MQ=255) gcctgccCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGata < 1:806517/140‑1 (MQ=255) gcctgccCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCATGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGata < 1:569165/140‑1 (MQ=255) cccTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTg > 1:822544/1‑140 (MQ=255) cccTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTg > 2:20389/1‑140 (MQ=255) cccTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTg < 1:133759/140‑1 (MQ=255) cccTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCCCGGACCTGAAAATTATCGGCGCATTGGTGGAAGGTATgggg > 2:133759/1‑138 (MQ=255) cccTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCCCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTg > 1:708611/1‑140 (MQ=255) cccTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGGTGATGACAACCCCCCGAACCCGAAACTTATCGGCGCACTGCTGGAAGATATGGTg > 2:1087620/1‑140 (MQ=255) cTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCa < 2:1053938/140‑1 (MQ=255) acagcGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGGCAACCGCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCa < 1:249709/136‑1 (MQ=255) aaTTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCCCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACAtgtg > 2:105298/1‑140 (MQ=255) aTTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTgg < 2:525111/140‑1 (MQ=255) ttttGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAAACTGAAAATTATCGGCGCATTGCTGGGAGATGTGGTGGAAAAAGTggg > 2:452267/1‑139 (MQ=255) tttGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGaa < 1:20389/140‑1 (MQ=255) gTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACtt > 1:494094/1‑140 (MQ=255) tcaCAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCg > 2:1081163/1‑140 (MQ=255) aacaCGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCa < 1:429276/140‑1 (MQ=255) cacGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATc < 1:113043/140‑1 (MQ=255) ccTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGa < 2:822544/140‑1 (MQ=255) gTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCCCGAACCTGAAACTTATCGGCGCATTTCTGGAAGAAATGGGGCAAAATGTGGAACTTTTCGATAGCGGGGATCAGGCGGTTGAACg > 2:406314/1‑140 (MQ=255) aaCCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACggg < 2:620921/140‑1 (MQ=255) aCCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGc < 1:1081163/140‑1 (MQ=255) aCCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGc < 2:999092/140‑1 (MQ=255) aCCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGc < 1:669812/140‑1 (MQ=255) ccGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCCCGAACCTGAAAATTATCGGCGCATTGCTGGGAGATATTGTGCAACATGTGGGAATTTTCGATAGCGGGGATCAGGGGGGTGAAAggggg > 2:789874/1‑138 (MQ=255) aaGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGa < 2:446333/140‑1 (MQ=255) gCAATGACAGTCATGGCGGTTGATGACAGCCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGa < 1:452267/140‑1 (MQ=255) cAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGAt < 2:517291/140‑1 (MQ=255) cAATGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGAt < 1:899580/140‑1 (MQ=255) aaTGACAGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGAtt < 2:1125041/140‑1 (MQ=255) aGTCATGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCt < 2:708611/140‑1 (MQ=255) tCATGGCGGTTGATGACAACCCCCCGAACCTGAAACTTATTGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGGGGGTGAAAGGGCGAAACAGATGCCGGTCGATTTGATCTTa > 2:946316/1‑140 (MQ=255) tGGCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATg < 2:962025/140‑1 (MQ=255) ggCGGTTGATGACAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATgg < 1:789874/140‑1 (MQ=255) tgatgaCAACCCCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTc < 1:82762/140‑1 (MQ=255) atgaCAACACCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGa > 2:479099/1‑133 (MQ=255) atgaCAACACCGCGAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGa < 1:479099/133‑1 (MQ=255) cgcgAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACa < 1:1087620/140‑1 (MQ=255) | GCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTGATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGCTGGAAGATATGGTGCAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA > NC_000913/2916955‑2917226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |