Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,082,708	T→G	T18P (ACC→CCC)	fdoH ←	formate dehydrogenase O subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,082,708	0	T	G	100.0%	280.5 / NA	72	T18P (ACC→CCC)	fdoH	formate dehydrogenase O subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (35/37);  total (35/37)

TTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGG  >  NC_000913/4082578‑4082843                                                                                                                                   |                                                                                                                                        ttGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGtt                                                                                                                                >  1:596306/1‑140 (MQ=255)       aCGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTcgc                                                                                                                          >  1:564983/1‑140 (MQ=255)           tATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGaa                                                                                                                      <  2:510248/140‑1 (MQ=255)              cgcgAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAacga                                                                                                                   >  1:1043734/1‑140 (MQ=255)                 gAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAAcgacga                                                                                                                <  1:776042/140‑1 (MQ=255)                 gAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAAcgacga                                                                                                                <  2:68334/140‑1 (MQ=255)                 gAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAAcgacga                                                                                                                <  2:1172847/140‑1 (MQ=255)                 gAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAAcgacga                                                                                                                >  2:658396/1‑140 (MQ=255)                     gTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGa                                                                                                            <  2:797592/140‑1 (MQ=255)                         ttCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATc                                                                                                        >  2:822954/1‑140 (MQ=255)                              ctctGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTgag                                                                                                   >  1:639276/1‑140 (MQ=255)                                   aaCACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGa                                                                                              >  2:466822/1‑140 (MQ=255)                                    acacGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGAt                                                                                             <  2:596306/140‑1 (MQ=255)                                     cacGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATa                                                                                            <  1:274424/140‑1 (MQ=255)                                     cacGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATa                                                                                            <  2:88420/140‑1 (MQ=255)                                      acGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATaa                                                                                           <  2:1001786/140‑1 (MQ=255)                                             cTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCatat                                                                                    >  1:469064/1‑140 (MQ=255)                                                  aGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCa                                                                                       >  2:64946/1‑132 (MQ=255)                                                  aGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCa                                                                                       <  1:64946/132‑1 (MQ=255)                                                  aGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtcgt                                                                               >  1:758122/1‑140 (MQ=255)                                                   ggCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtcgtc                                                                              <  1:658396/140‑1 (MQ=255)                                                    gCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATg                                                                                   <  2:248062/134‑1 (MQ=255)                                                    gCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATg                                                                                   >  1:248062/1‑134 (MQ=255)                                                        tACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGt                                                                         >  1:191809/1‑140 (MQ=255)                                                           aGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTac                                                                      <  1:941761/140‑1 (MQ=255)                                                              cGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACAcc                                                                   >  1:923926/1‑140 (MQ=255)                                                                aTACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCtt                                                                 >  1:16038/1‑140 (MQ=255)                                                                       tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCcaca                                                          <  1:822954/140‑1 (MQ=255)                                                                         gtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACAtt                                                        <  1:592388/140‑1 (MQ=255)                                                                           gaCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTca                                                      >  2:442832/2‑140 (MQ=255)                                                                           aaCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTac                                                                      <  1:588559/124‑1 (MQ=255)                                                                           aaCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTac                                                                      >  2:588559/1‑124 (MQ=255)                                                                           aaCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTca                                                      >  2:1131057/1‑140 (MQ=255)                                                                              gTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaa                                                   >  2:183293/1‑140 (MQ=255)                                                                               tCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaag                                                  >  2:471188/1‑140 (MQ=255)                                                                                cGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagg                                                 >  1:1045062/1‑140 (MQ=255)                                                                                cGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagg                                                 >  2:1125003/1‑140 (MQ=255)                                                                                 gATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga                                                <  1:289762/140‑1 (MQ=255)                                                                                 gATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga                                                <  2:16038/140‑1 (MQ=255)                                                                                 gATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga                                                <  1:509642/140‑1 (MQ=255)                                                                                  aTGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCTGTTACACCTTGTCCACATTCACaaggaa                                               >  2:1048983/1‑140 (MQ=255)                                                                                  aTGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaa                                               >  2:511327/1‑140 (MQ=255)                                                                                  aTGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaa                                               <  2:923926/140‑1 (MQ=255)                                                                                   tGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaag                                              <  2:1045062/140‑1 (MQ=255)                                                                                      gTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGAc                                           <  1:554256/140‑1 (MQ=255)                                                                                            ccACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAAc                                     <  1:442832/140‑1 (MQ=255)                                                                                              aCTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTc                                   <  1:471188/140‑1 (MQ=255)                                                                                              aCTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTc                                   <  2:469064/140‑1 (MQ=255)                                                                                               cttcttCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTcc                                  >  2:733002/1‑140 (MQ=255)                                                                                                ttcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCg                                 >  2:193151/1‑140 (MQ=255)                                                                                                  cttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGc                               <  1:1048983/140‑1 (MQ=255)                                                                                                      cTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCt                           <  2:758122/140‑1 (MQ=255)                                                                                                          aaGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCgt                       <  1:193151/140‑1 (MQ=255)                                                                                                           aGTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCgtg                      <  1:183293/140‑1 (MQ=255)                                                                                                            gTCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCgtgt                     >  1:72209/1‑140 (MQ=255)                                                                                                             tCCCGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGtt                    >  2:862642/1‑140 (MQ=255)                                                                                                               ccGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCg                  >  2:996599/1‑140 (MQ=255)                                                                                                               ccGCGCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCg                  >  1:422871/1‑140 (MQ=255)                                                                                                                cgcgCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGc                 <  2:422871/140‑1 (MQ=255)                                                                                                                cgcgCCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGc                 <  1:1131057/140‑1 (MQ=255)                                                                                                                 gcgcCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCa                <  1:862642/140‑1 (MQ=255)                                                                                                                  cgcCTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCAt               >  2:312347/1‑140 (MQ=255)                                                                                                                    ccTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCa             >  1:212411/1‑140 (MQ=255)                                                                                                                    ccTGAGGCGCGGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCa             >  1:1049715/1‑140 (MQ=255)                                                                                                                            gcgGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGaa     <  2:649361/140‑1 (MQ=255)                                                                                                                            gcgGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGaa     <  2:72209/140‑1 (MQ=255)                                                                                                                            gcgGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGaa     <  2:1043734/140‑1 (MQ=255)                                                                                                                            gcgGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGaa     <  2:857261/140‑1 (MQ=255)                                                                                                                             cgGGGGGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAAt    >  1:457616/1‑140 (MQ=255)                                                                                                                               ggggGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTc                   <  2:600877/123‑1 (MQ=255)                                                                                                                               ggggGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTc                   >  1:600877/1‑123 (MQ=255)                                                                                                                               ggggGCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATgg  >  2:163555/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        TTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGG  >  NC_000913/4082578‑4082843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑