Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	312,031	A→C	intergenic (‑695/‑81)	ecpR ← / → ykgL	DNA‑binding transcriptional dual regulator MatA/uncharacterized protein YkgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	312,031	0	A	C	100.0%	334.0 / NA	85	intergenic (‑695/‑81)	ecpR/ykgL	DNA‑binding transcriptional dual regulator MatA/uncharacterized protein YkgL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (39/46);  total (39/46)

AGATTAAGTGAAGTTAAACGAAGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGA  >  NC_000913/311895‑312165                                                                                                                                         |                                                                                                                                       aGATTAAGTGAAGTTAAACGAAGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGAc                                                                                                                                     <  2:23484/140‑1 (MQ=255) aGATTAAGTGAAGTTAAACGAAGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGAc                                                                                                                                     <  2:812905/140‑1 (MQ=255)   aTTAAGTGAAGTTAAACGAAGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTg                                                                                                                                   <  1:858164/140‑1 (MQ=255)           aaGTTAAACGAAGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCtgtg                                                                                                                           <  2:487321/140‑1 (MQ=255)             gTTAAACGAAGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTg                                                                                                                                   <  2:606465/130‑1 (MQ=255)             gTTAAACGAAGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTg                                                                                                                                   >  1:606465/1‑130 (MQ=255)              ttAAACGAAGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCg                                                                                                                        >  2:385698/1‑140 (MQ=255)                 aaCGAAGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTaa                                                                                                                     <  1:784306/140‑1 (MQ=255)                 aaCGAAGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGCGGCGTac                                                                                                                     <  2:880642/140‑2 (MQ=255)                  aCGAAGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTaaa                                                                                                                    <  1:385698/140‑1 (MQ=255)                     aaGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGt                                                                                                                 <  2:462351/140‑1 (MQ=255)                      aGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTaa                                                                                                                     <  2:460930/135‑1 (MQ=255)                      aGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTaa                                                                                                                     >  1:460930/1‑135 (MQ=255)                       ggCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGttt                                                                                                               >  1:846671/1‑140 (MQ=255)                       ggCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGttt                                                                                                               >  1:146783/1‑140 (MQ=255)                        gCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGtttt                                                                                                              <  1:239920/140‑1 (MQ=255)                        gCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGtttt                                                                                                              >  2:583458/1‑140 (MQ=255)                        gCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGtttt                                                                                                              >  1:130161/1‑140 (MQ=255)                        gCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGtttt                                                                                                              >  2:314585/1‑140 (MQ=255)                              aGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAAc                                                                                                                   >  2:381711/1‑129 (MQ=255)                              aGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAAc                                                                                                                   <  1:381711/129‑1 (MQ=255)                                 tGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGt                                                                                                     <  1:484419/140‑1 (MQ=255)                                  gTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGtttt                                                                                                              >  2:952997/1‑130 (MQ=255)                                  gTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGtttt                                                                                                              <  1:952997/130‑1 (MQ=255)                                        ggAACGATGGCCTTTTTGTCA‑GGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAg                                                                                             <  1:895299/140‑1 (MQ=255)                                         gAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAg                                                                                             >  1:462145/1‑140 (MQ=255)                                          aaCGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGa                                                                                            >  1:1119394/1‑140 (MQ=255)                                           aCGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGAt                                                                                           >  2:669916/1‑140 (MQ=255)                                            cGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGAtt                                                                                          <  1:809434/140‑1 (MQ=255)                                               tGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAg                                                                                                      >  2:184169/1‑125 (MQ=255)                                               tGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAg                                                                                                      <  1:184169/125‑1 (MQ=255)                                               tGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTAc                                                                                       >  2:1119211/1‑140 (MQ=255)                                               tGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTAc                                                                                       >  2:310342/1‑140 (MQ=255)                                                      tttGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCaa                                                                                                <  2:1077658/124‑1 (MQ=255)                                                      tttGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCaa                                                                                                >  1:1077658/1‑124 (MQ=255)                                                        tGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTat                                                                              <  2:846671/140‑1 (MQ=255)                                                             gggCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCt                                                                         >  1:328666/1‑140 (MQ=255)                                                              ggCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCtt                                                                        >  2:853471/1‑140 (MQ=255)                                                                cACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTaa                                                                      <  2:738777/140‑1 (MQ=255)                                                                aaaagCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTaa                                                                      <  1:153959/136‑1 (MQ=255)                                                                    gCATTCAGTTATCTGTGCGCGTTGCTCTAACTAGCACTGGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACCTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGt                                                                  <  2:267128/140‑1 (MQ=255)                                                                       ttCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTg                                                               <  2:1137408/140‑1 (MQ=255)                                                                        tCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGt                                                                  <  1:947060/136‑1 (MQ=255)                                                                        tCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGt                                                                  >  2:947060/1‑136 (MQ=255)                                                                        tCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGa                                                              >  1:389899/1‑140 (MQ=255)                                                                          aGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGt                                                            >  2:1172568/1‑140 (MQ=255)                                                                             tATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGaa                                                         >  1:398412/1‑140 (MQ=255)                                                                               tCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGaaaa                                                       >  2:1022632/1‑140 (MQ=255)                                                                               tCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGaaaa                                                       >  2:249542/1‑140 (MQ=255)                                                                                  gtgCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGc                                                    >  2:760466/1‑140 (MQ=255)                                                                                   tgCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCa                                                   <  1:669916/140‑1 (MQ=255)                                                                                    gcgcGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAg                                                  >  2:360345/1‑140 (MQ=255)                                                                                    gcgcGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAg                                                  >  2:806735/1‑140 (MQ=255)                                                                                    gcgcGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAg                                                  >  1:152664/1‑140 (MQ=255)                                                                                     cgcgTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAgg                                                 <  2:970509/140‑1 (MQ=255)                                                                                      gcgTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGc                                                >  2:1051283/1‑140 (MQ=255)                                                                                        gTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTg                                              <  1:1051283/140‑1 (MQ=255)                                                                                         ttACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTgc                                             <  1:1022632/140‑1 (MQ=255)                                                                                         ttACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTgc                                             <  1:193271/140‑1 (MQ=255)                                                                                          tACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTgcg                                            <  2:398412/140‑1 (MQ=255)                                                                                             tctAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGcgccg                                         >  1:233757/1‑140 (MQ=255)                                                                                               tAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAG‑GAAAATGCAGGCTGCGCCGCaa                                      <  2:382998/140‑1 (MQ=255)                                                                                                aaCTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCaa                                      <  2:1124482/140‑1 (MQ=255)                                                                                                aaCTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCaa                                      <  1:360345/140‑1 (MQ=255)                                                                                                aaCTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCaa                                      <  1:676264/140‑1 (MQ=255)                                                                                                aaCTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCaa                                      >  1:257690/1‑140 (MQ=255)                                                                                                    ggCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAggg                                  >  2:283917/1‑140 (MQ=255)                                                                                                      cACTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGggag                                >  2:703814/1‑140 (MQ=255)                                                                                                       aCTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGggagg                               >  2:174480/1‑140 (MQ=255)                                                                                                       aCTCGAACGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGggagg                               >  1:566113/1‑140 (MQ=255)                                                                                                            aaCGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACt                          <  1:583458/140‑1 (MQ=255)                                                                                                              cGTCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGa                        <  2:925730/140‑1 (MQ=255)                                                                                                                tCGGCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGt                      >  1:57417/1‑140 (MQ=255)                                                                                                                   gCGACGGGGTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGc                   <  1:703814/140‑1 (MQ=255)                                                                                                                        ggggTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGtt              <  2:152664/140‑1 (MQ=255)                                                                                                                        ggggTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGtt              <  2:716369/140‑1 (MQ=255)                                                                                                                        ggggTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGtt              <  1:760466/140‑1 (MQ=255)                                                                                                                        ggggTTACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGtt              <  1:853471/140‑1 (MQ=255)                                                                                                                            ttACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGt          <  1:806735/140‑1 (MQ=255)                                                                                                                             tACCGGTGGATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTc         <  1:174480/140‑1 (MQ=255)                                                                                                                                    ggATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAaga  >  1:601729/1‑140 (MQ=255)                                                                                                                                    ggATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAaga  >  1:66209/1‑140 (MQ=255)                                                                                                                                    ggATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAaga  <  2:566113/140‑1 (MQ=255)                                                                                                                                    ggATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAaga  <  1:1026372/140‑1 (MQ=255)                                                                                                                                    ggATCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAaga  <  2:146783/140‑1 (MQ=255)                                                                                                                                      aTCCGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGtt              <  2:1177638/126‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       AGATTAAGTGAAGTTAAACGAAGGCTGCCAGCTGTCACAGGAACGATGGCCTTTTTGTCAGGGCACTGCATTCAGTTATCTGTGCGCGTTACTCTAACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGA  >  NC_000913/311895‑312165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑