Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,111,7470GT31.2% 74.6 / 16.9 32N156K (AAC→AAA) yiiQDUF1454 domain‑containing protein YiiQ
Reads supporting (aligned to +/- strand):  ref base G (21/1);  new base T (0/10);  total (21/11)
Fisher's exact test for biased strand distribution p-value = 1.71e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATG  >  NC_000913/4111612‑4111885
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ttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGtttt                                                                                                                                        >  2:238690/1‑140 (MQ=255)
ttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGtttt                                                                                                                                        >  2:128199/1‑140 (MQ=255)
          ttCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGt                                                                                                                              >  2:15806/1‑140 (MQ=255)
               aCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagta                                                                                                                         >  1:763055/1‑140 (MQ=255)
                  ttCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCg                                                                                                                      >  1:631802/1‑140 (MQ=255)
                   tCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGa                                                                                                                     >  1:252034/1‑140 (MQ=255)
                   ccAAGCGTTTCAGATGGCGCCAGCTAAATCGTTTAAACAGCGAAGGCCACCCCTTTTT‑CGCCGTTGCCCGCGACAACAAACCGCAGTCCCCTTTCTGTCTGGGTGTAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGa                                                                                                                     <  2:159354/139‑1 (MQ=255)
                      aGCGATTAAGATAGCCCCAGTTAAATCGTTTCAACAGCGAGGGCCACCCCCTTTC‑CGCCTTTGTCCGCAAAAACATAACGCGGTGCCCCTTCTGTCTGGGTTTAATAATTTTTTTTTTCCCCCGCCGTTAGTAGCGACTg                                                                                                                  <  2:763055/140‑1 (MQ=255)
                           ttCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtt                                                                                                             >  1:491187/1‑140 (MQ=255)
                             cAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtttt                                                                                                           >  1:712970/1‑140 (MQ=255)
                                     gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                   >  1:425837/1‑140 (MQ=255)
                                                   ttCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                     >  2:563007/1‑140 (MQ=255)
                                                   taaaaCAGCGAAGGTCACCCCCTTTC‑CGCCTTTGTCCGCGAAAACATACCGCGGTCCCCTTTCTGTCTGGGTGTAATACTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                     <  2:278990/137‑1 (MQ=255)
                                                             aaGGTCCGCTTCTTTT‑CGCCTTTGCCCGCAACAAAACACCGCAGTGCCCCTCCGTTGGGGGTTTAATTTTGTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           <  2:425837/140‑1 (MQ=255)
                                                                gTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAg                                                                        >  2:951086/1‑140 (MQ=255)
                                                                     ccccTTTTCCGCCGTTGCCCGAAAAAAAAAACCGCAGTCCCCTTTTTTTCTGGGTAAAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGtgtg                                                                    <  2:100533/140‑1 (MQ=255)
                                                                          ttt‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATc                                                              >  1:108499/1‑140 (MQ=255)
                                                                               gCCGTTGCCCGCAAAAACATAACGCAGTCCCCCTTCTGTCTGGGTTTAATACTTTTTGTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCg                                                          <  2:786699/140‑1 (MQ=255)
                                                                                  tttccccGCAACAACATACCGCAGTGCCCCTTCTGTCTGGGTTTAATATCTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg                                                       <  2:252034/135‑1 (MQ=255)
                                                                                  tttGCCCGCAACAACATAACGCAGTCCCCCTTCTGTCTCGGTGTAATACCTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg                                                       <  1:675860/139‑1 (MQ=255)
                                                                                       ccGCGAAAAAATAACGCGGTCCCCCTTCTGTCTGGGTGAAATAATTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTAATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATg                                                  <  1:190571/140‑1 (MQ=255)
                                                                                          cgACAACATACCGCAGTCCCCCTTCTGTCTGGGTTTAATATCTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTAt                                               <  1:207805/140‑1 (MQ=255)
                                                                                                caTACCGCAGTCCCCCTTCTGTCTGGGTTTAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGa                                         <  2:712970/140‑1 (MQ=255)
                                                                                                caTAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGa                                         >  1:816683/1‑140 (MQ=255)
                                                                                                     cGCAGTCCCCCTTCTGTCTGGGTGTAATAACTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgcttt                                    <  1:238690/140‑1 (MQ=255)
                                                                                                        aGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgc                                 >  1:529675/1‑140 (MQ=255)
                                                                                                          tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  2:458433/1‑140 (MQ=255)
                                                                                                          tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  2:600224/1‑140 (MQ=255)
                                                                                                          tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  1:576093/1‑140 (MQ=255)
                                                                                                           gCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgcttt                              >  1:739876/1‑140 (MQ=255)
                                                                                                           gCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgcttt                              >  2:690116/1‑140 (MQ=255)
                                                                                                               cTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTTAGCTTTCGCTTTcgcg                          >  1:242758/1‑140 (MQ=255)
                                                                                                                         ggTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGct                >  2:505577/1‑140 (MQ=255)
                                                                                                                                   ttttttttttCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTTAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg      <  2:242758/138‑1 (MQ=255)
                                                                                                                                     ttttttttCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGcc          <  2:241765/134‑1 (MQ=255)
                                                                                                                                     tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGcc          >  1:241765/1‑134 (MQ=255)
                                                                                                                                       tgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg  >  2:807163/1‑140 (MQ=255)
                                                                                                                                       tgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg  >  1:718283/1‑140 (MQ=255)
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TTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATG  >  NC_000913/4111612‑4111885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑