| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,521,833 | C→G | L411L (CTG→CTC) | yncD ← | putative TonB‑dependent outer membrane receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,521,833 | 0 | C | G | 98.4% | 228.3 / ‑5.2 | 61 | L411L (CTG→CTC) | yncD | putative TonB‑dependent outer membrane receptor |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (30/30); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTA > NC_000913/1521702‑1521959 | tatTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTc < 1:761807/140‑1 (MQ=255) atTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCg < 1:741777/140‑1 (MQ=255) ttttAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGc < 1:3799/140‑1 (MQ=255) ttAACGAACCGGCAGGTAGCCATTTATTATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTc < 1:667830/140‑1 (MQ=255) aaCGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTc > 1:151075/1‑138 (MQ=255) aaCGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTc < 2:151075/138‑1 (MQ=255) aaCGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAg < 2:729477/140‑1 (MQ=255) aaCGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAg < 1:940142/140‑1 (MQ=255) agacccGGCAGGTAGCCATTTATGCTCACTGGCATCCCCGCTGTCATCGCCGTTACCCGGAGTACCGTAATGGTCGTTGGAATCAAACCTCACGGAGCTGTAGCGCACGCCCGCATCCAGCGACAGTTTTTCGCTCAGCt < 2:674940/136‑1 (MQ=255) cGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCGGCTGCCAc < 1:612527/140‑1 (MQ=255) gCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTg < 1:338252/140‑1 (MQ=255) cAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGc > 2:43321/1‑140 (MQ=255) cAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGc > 2:364369/1‑140 (MQ=255) tAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCt < 2:175542/140‑1 (MQ=255) tttATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAAt > 2:365704/1‑140 (MQ=255) aTGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAgg > 2:142265/1‑140 (MQ=255) aTGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAgg > 2:902381/1‑140 (MQ=255) tGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAggg > 1:736273/1‑140 (MQ=255) aTAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGAt > 1:463197/1‑140 (MQ=255) tAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATc > 1:842387/1‑140 (MQ=255) aCTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGa < 1:248514/140‑1 (MQ=255) aCTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGa < 2:842387/140‑1 (MQ=255) cTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGAt > 2:34038/1‑140 (MQ=255) tGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATg < 1:142265/140‑1 (MQ=255) gCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGtt > 2:684532/1‑140 (MQ=255) cATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTc > 1:480158/1‑140 (MQ=255) cGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATc > 1:258967/1‑140 (MQ=255) tGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGa > 2:420692/1‑140 (MQ=255) gCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGtt > 1:798696/1‑140 (MQ=255) tACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTtcgtcg > 2:576751/1‑140 (MQ=255) ccGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGa < 2:798696/140‑1 (MQ=255) ccGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGa < 1:365704/140‑1 (MQ=255) ccGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGa < 2:480158/140‑1 (MQ=255) cGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGAc < 1:216040/140‑1 (MQ=255) gTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCaa > 1:463328/1‑140 (MQ=255) tAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAAc > 1:634948/1‑140 (MQ=255) aCGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTc < 1:34038/140‑1 (MQ=255) tGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCttt > 1:592699/1‑140 (MQ=255) gTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCttttt > 2:939236/1‑140 (MQ=255) cGTTGGAAACAAACCTAACGGAGCGGTAGCGCTCGCCATCCTCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGTGTCTGTAAATAGGGATCGATGTTCCACATCATATTGCGTTCGTCGCGACGCAACTCACCTTTTTGc < 2:390315/140‑1 (MQ=255) gTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGcc > 2:65953/1‑140 (MQ=255) tGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTc > 2:133554/1‑127 (MQ=255) tGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTc < 1:133554/127‑1 (MQ=255) tGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCg > 2:247357/1‑140 (MQ=255) tGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCg > 1:199663/1‑140 (MQ=255) ccACACGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCa < 1:576751/140‑1 (MQ=255) cacGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGc < 1:364369/140‑1 (MQ=255) cacGGAGCTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGc < 1:43321/140‑1 (MQ=255) cTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTAtt < 2:33824/140‑1 (MQ=255) cTGTAGCGCACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTAtt < 1:684532/140‑1 (MQ=255) cgcACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTGACCTTTTTGCCCGTACTCCGGCATGCCGCTATTAAGGCg < 2:634948/140‑1 (MQ=255) cgcACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCg > 2:265009/1‑140 (MQ=255) cACGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGa > 2:682619/1‑140 (MQ=255) cGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAg < 1:682619/140‑1 (MQ=255) cGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAg < 1:265009/140‑1 (MQ=255) cGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAg < 2:199663/140‑1 (MQ=255) cGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAg > 1:511150/1‑140 (MQ=255) cGCCAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTACCCGTACTCCGGCATGCCGCTATTCAGGCGGAAg < 2:463197/140‑1 (MQ=255) cAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCATATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTa > 2:938931/1‑140 (MQ=255) cAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGt < 2:655693/138‑1 (MQ=255) cAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGt > 1:344726/1‑138 (MQ=255) cAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGt < 2:344726/138‑1 (MQ=255) cAGCATCCAGCGAGAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGt > 1:655693/1‑138 (MQ=255) | TATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCGTACTCCGGCATGCCGCTATTCAGGCGGAAGTTA > NC_000913/1521702‑1521959 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |