| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 50,988 | +CG | coding (235/843 nt) | apaH ← | diadenosine tetraphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 50,985 | 1 | . | G | 92.6% | 187.6 / 6.7 | 54 | coding (238/843 nt) | apaH | diadenosine tetraphosphatase |
| Reads supporting (aligned to +/- strand): ref base . (3/1); new base G (25/25); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 50,985 | 2 | . | C | 92.6% | 186.7 / 6.8 | 54 | coding (238/843 nt) | apaH | diadenosine tetraphosphatase |
| Reads supporting (aligned to +/- strand): ref base . (3/1); new base C (25/25); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGCGTGATCCCTGCGTGGGCCATCACCAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATT‑‑GCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAGCGCAGAACATCCAGCGAACCCGGCCCGCGCGCGACCAG > NC_000913/50847‑51111 || gcggcgTGATCCCTGCGTGGGCCATCACCAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATT‑‑g < 2:249864/140‑1 (MQ=255) cggcgTGATCCCTGCGTGGGCCATCACCAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATT‑‑Gc > 2:709766/1‑140 (MQ=255) cgTGATCCCTGCGTGGGCCATCACCAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATT‑‑GCgcg > 2:878395/1‑138 (MQ=255) gTGGGCCATCACCAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGc > 1:664798/1‑140 (MQ=255) ggCCATCACCAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCaaa > 1:434609/1‑140 (MQ=255) ggCCATCACCAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCACCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCaaa > 2:401778/1‑140 (MQ=255) aTCACCAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATAcc > 2:570448/1‑140 (MQ=255) aTCACCAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATAcc > 2:866448/1‑140 (MQ=255) tCACCAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCg < 1:868261/140‑1 (MQ=255) aCCAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGcc < 2:754767/140‑1 (MQ=255) cAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCcag < 1:709766/140‑1 (MQ=255) cAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCcag < 1:878395/140‑1 (MQ=255) cAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCcag < 1:563066/140‑1 (MQ=255) cTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCcagcag > 2:232327/1‑140 (MQ=255) ttttCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCcag < 1:432274/135‑1 (MQ=255) ttttCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCcag > 2:432274/1‑135 (MQ=255) ctTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAgcagatgcagat < 2:434609/140‑1 (MQ=255) tcgtcgATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCcagca > 1:1000883/1‑129 (MQ=255) tcgtcgATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCcagca < 2:1000883/129‑1 (MQ=255) tttGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATT‑‑GCGCTGCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGc > 1:384036/1‑140 (MQ=255) ttGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGcc < 1:866448/140‑1 (MQ=255) tGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGccc < 2:761603/140‑1 (MQ=255) tGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATT‑‑GCGCTGCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGccc < 2:384036/140‑1 (MQ=255) agGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAg > 2:924817/1‑140 (MQ=255) agGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAg > 1:1063390/1‑140 (MQ=255) gCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGAc > 1:183055/1‑140 (MQ=255) gCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGAc < 2:53824/140‑1 (MQ=255) tGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGt < 2:8738/140‑1 (MQ=255) cgccgcAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACg > 1:770056/1‑140 (MQ=255) gggcagcaAGTTAAGCAGCCCGGCGGCGTCGGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCt < 2:997352/138‑1 (MQ=255) ccgcAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCt < 2:448081/140‑1 (MQ=255) gcAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGt < 1:232327/140‑1 (MQ=255) cAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTc > 1:26122/1‑140 (MQ=255) cAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCt < 2:981859/140‑1 (MQ=255) gTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGTAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTaa < 1:570448/140‑1 (MQ=255) gTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTaa < 2:1063390/140‑1 (MQ=255) gcagcTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGAtt < 1:581604/140‑1 (MQ=255) cagcTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGACCGTGATTGCCCCGCACCAGACGTACGCGGTTGTCTAATGaact > 1:1059375/1‑137 (MQ=255) agcTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTc > 1:908183/1‑140 (MQ=255) tcgtcgGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTcaca < 1:97379/140‑1 (MQ=255) cgtcgGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACAt > 2:867535/1‑140 (MQ=255) tcgGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAg < 1:637231/140‑1 (MQ=255) cgGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAgc < 1:924220/140‑1 (MQ=255) cgGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCACAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAgc > 2:686254/1‑140 (MQ=255) tCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAGCGCAGa > 2:904678/1‑140 (MQ=255) ggCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAGCGCAGAACa > 2:744865/1‑140 (MQ=255) ccAGCAGCGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAGCGCAGAACATCCAGCg > 2:24994/1‑140 (MQ=255) cagcGGTGTCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAGCGCAGAACATCCAGCGAAcc < 1:867535/140‑1 (MQ=255) gtgtCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAGCGCAGAACATCCAGCGAACCCGGcc > 2:402662/1‑140 (MQ=255) gtCAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAGCGCAGAACATCCAGCGAACCCGGCCcg < 2:67734/140‑1 (MQ=255) cAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATCCCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAGCGCAGAACATCCAGCGAACCCGGCCcgcg < 2:5733/140‑1 (MQ=255) cAGGCGATCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAGCGCAGAACATCCAGCGAACCCGGCCcgcg > 2:201500/1‑140 (MQ=255) aTCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAGCGCAGAACATCCAGCGAACCCGGCCCGCGCGCGAc > 1:220308/1‑140 (MQ=255) aTCTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGGGATTGCCCAGCACCATACGTACGCTGTCGCCTAAGGATTTCACATAGCGCAGAACATCCAGCTAACCCTGCCCGCGCGCGAc > 2:986193/1‑140 (MQ=255) cTTTCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAGCGCAGAACATCCAGCGAACCCGGCCCGCGCGCGACCa < 2:26122/140‑1 (MQ=255) tttCGGTTTATTGCGCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAGCGCAGAACATCCAGCGAACCCGGCCCGCGCGCGACCAg > 1:938347/1‑140 (MQ=255) || GCGGCGTGATCCCTGCGTGGGCCATCACCAGCTTTTTCTCTTCGTCGATTTGCAGCAGAGGCTGGCGCCGCAGCCAGTTAAGCAGCTCGTCGGCATCCGGCGCTTCCAGCAGCGGTGTCAGGCGATCTTTCGGTTTATT‑‑GCG‑‑GCTGATCCCGGCAAATACCGCCAGCAGATGCAGATCGTGATTGCCCAGCACCAGACGTACGCTGTCGCCTAAGGATTTCACATAGCGCAGAACATCCAGCGAACCCGGCCCGCGCGCGACCAG > NC_000913/50847‑51111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |