Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,963,333	T→C	E128E (GAA→GAG)	ppdB ←	conserved protein PpdB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,963,333	0	T	C	100.0%	280.2 / NA	72	E128E (GAA→GAG)	ppdB	conserved protein PpdB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/38);  total (34/38)

TGCGGTTCAGACTTACTGGCAGCACGCATATTAACCGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACA  >  NC_000913/2963199‑2963464                                                                                                                                         |                                                                                                                                    tGCGGTTCAGACTTACTGGCAGCACGCATATTAACCGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTcaca                                                                                                                                <  1:960870/140‑1 (MQ=255)  gCGGTTCAGACTTACTGGCAGCACGCATATTAACCGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAg                                                                                                                               >  2:577051/1‑140 (MQ=255)        cAGACTTACTGGCAGCACGCATATTAACCGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTc                                                                                                                         >  2:154614/1‑140 (MQ=255)        cAGACTTACTGGCAGCACGCATATTAACCGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTc                                                                                                                         >  1:338717/1‑140 (MQ=255)        cAGACTTACTGGCAGCACGCATATTAACCGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTc                                                                                                                         >  2:1012096/1‑140 (MQ=255)          gACTTACTGGCAGCACGCATATTAACCGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGc                                                                                                                       <  1:566171/140‑1 (MQ=255)                       caCGCATATTAACCGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGtt                                                                                                          >  1:72045/1‑140 (MQ=255)                          gCATATTAACCGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTcc                                                                                                       >  2:923117/1‑140 (MQ=255)                             tatTAACCGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGc                                                                                                    >  1:633388/1‑140 (MQ=255)                             tatTAACCGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGc                                                                                                    <  1:577051/140‑1 (MQ=255)                                tAACCGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGcaca                                                                                                 <  2:528156/140‑1 (MQ=255)                                   ccGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGc                                                                                              <  1:310967/140‑1 (MQ=255)                                    cGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCt                                                                                             >  1:782474/1‑140 (MQ=255)                                    cGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCt                                                                                             >  2:955530/1‑140 (MQ=255)                                    cGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCt                                                                                             >  1:67820/1‑140 (MQ=255)                                     gTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTc                                                                                            <  1:112909/140‑1 (MQ=255)                                      tCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTcc                                                                                           >  1:772359/1‑140 (MQ=255)                                        aaCACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCtt                                                                                         >  2:475634/1‑140 (MQ=255)                                        aaCACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCtt                                                                                         >  2:920815/1‑140 (MQ=255)                                         acacCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTc                                                                                        <  1:841462/140‑1 (MQ=255)                                            ccGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGa                                                                                     <  2:72045/140‑1 (MQ=255)                                             cGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGAc                                                                                    <  2:633388/140‑1 (MQ=255)                                             cGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGAc                                                                                    <  1:665441/140‑1 (MQ=255)                                             cGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGAc                                                                                    >  1:333686/1‑140 (MQ=255)                                             cGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGAc                                                                                    <  1:154614/140‑1 (MQ=255)                                             cGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGAc                                                                                    <  2:333686/140‑1 (MQ=255)                                              ggCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACg                                                                                   >  1:54174/1‑140 (MQ=255)                                              ggCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACg                                                                                   >  2:406178/1‑140 (MQ=255)                                                      gCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCaa                                                                           <  2:652900/140‑1 (MQ=255)                                                          cTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTg                                                                       <  2:338717/140‑1 (MQ=255)                                                            gACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGt                                                                     <  1:409420/140‑1 (MQ=255)                                                                 ccTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGAc                                                                <  1:583067/140‑1 (MQ=255)                                                                     aCGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCtt                                                            >  1:1028714/1‑140 (MQ=255)                                                                        tACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTa                                                         <  1:923117/140‑1 (MQ=255)                                                                         aCGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTAc                                                        >  1:817364/1‑140 (MQ=255)                                                                            aCCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCgg                                                     >  1:324763/1‑140 (MQ=255)                                                                            aCCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCgg                                                     >  2:937019/1‑140 (MQ=255)                                                                             ccTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGt                                                    >  2:654384/1‑140 (MQ=255)                                                                                gAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTcg                                                 <  1:937019/140‑1 (MQ=255)                                                                                gAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTcg                                                 <  2:1028714/140‑1 (MQ=255)                                                                                  aaaGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTAAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTcgcg                                               <  2:327240/140‑1 (MQ=255)                                                                                    aGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGAt                                             <  2:54174/140‑1 (MQ=255)                                                                                    aGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGAt                                             <  2:970181/140‑1 (MQ=255)                                                                                        tCGATAATGATGGCATCCGGATTAGTGTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATcc                                           >  2:491136/1‑140 (MQ=255)                                                                                        tCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCa                                         <  1:654384/140‑1 (MQ=255)                                                                                        tCGATAATGATGGAATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCa                                         <  1:383636/140‑1 (MQ=255)                                                                                           aTAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGaa                                      >  2:856801/1‑139 (MQ=255)                                                                                           aTAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGAt                                      >  2:500220/1‑140 (MQ=255)                                                                                               tgatgGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCg                                  >  2:668256/1‑140 (MQ=255)                                                                                                  tgGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTa                               <  1:500220/140‑1 (MQ=255)                                                                                                           gATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCa                      <  2:240907/140‑1 (MQ=255)                                                                                                           gATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCa                      >  2:900783/1‑140 (MQ=255)                                                                                                           gATTA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCa                      <  2:778492/140‑1 (MQ=255)                                                                                                              tA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATcc                   <  1:475634/140‑1 (MQ=255)                                                                                                              tA‑‑GTGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCACATcc                   <  2:772359/140‑1 (MQ=255)                                                                                                                   tGACTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCAc                <  1:920815/140‑1 (MQ=255)                                                                                                                     aCTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTg              <  1:406178/140‑1 (MQ=255)                                                                                                                     aCTTTATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCACGATCCCAGATACCGTTACTGTTCGCATCCCACTg              <  1:1012096/140‑1 (MQ=255)                                                                                                                       tttATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGca            >  1:503104/1‑140 (MQ=255)                                                                                                                        ttATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCg                        >  1:656484/1‑127 (MQ=255)                                                                                                                        ttATCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCg                        <  2:656484/127‑1 (MQ=255)                                                                                                                           tCCCAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGTGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAAt        >  1:150759/1‑140 (MQ=255)                                                                                                                             ccAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGa      <  1:83030/140‑1 (MQ=255)                                                                                                                             ccAGCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGa      <  2:301855/140‑1 (MQ=255)                                                                                                                                gCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGacac   >  1:572396/1‑140 (MQ=255)                                                                                                                                gCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGa      <  2:118467/137‑1 (MQ=255)                                                                                                                                gCCCTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGa      >  1:118467/1‑137 (MQ=255)                                                                                                                                 cccTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGAcaca  >  2:820308/1‑140 (MQ=255)                                                                                                                                 cccTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGAcaca  <  1:955530/140‑1 (MQ=255)                                                                                                                                 cccTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGAcaca  >  2:938410/1‑140 (MQ=255)                                                                                                                                 cccTTACCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGCCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGAcaca  >  1:449982/1‑140 (MQ=255)                                                                                                                                      aCCCTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGaca    <  2:63319/133‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TGCGGTTCAGACTTACTGGCAGCACGCATATTAACCGTCAACACCGGCGAGAAGCCGCTGACATCCTGACGTACGACCTGAAAAGTGTCGATAATGATGGCATCCGGATTA‑‑GTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACA  >  NC_000913/2963199‑2963464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑