Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,035,052	A→G	R41R (CGT→CGC)	lysS ←	lysine‑‑tRNA ligase, constitutive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,035,052	0	A	G	100.0%	204.1 / NA	53	R41R (CGT→CGC)	lysS	lysine‑‑tRNA ligase, constitutive
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/24);  total (29/24)

TAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATA  >  NC_000913/3034916‑3035188                                                                                                                                         |                                                                                                                                         tAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGcggcgg                                                                                                                                       >  1:677656/1‑140 (MQ=255)  aaCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGa                                                                                                                                      <  1:212276/140‑1 (MQ=255)           cGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCg                                                                                                                             >  2:688290/1‑140 (MQ=255)                       aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATcc                                                                                                                 >  1:549094/1‑140 (MQ=255)                            gacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGc                                                                                                            >  2:548612/1‑140 (MQ=255)                                 gTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgcg                                                                                                       >  1:791559/1‑140 (MQ=255)                                 gTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgcg                                                                                                       >  1:243720/1‑140 (MQ=255)                                 gTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgcg                                                                                                       >  1:247589/1‑140 (MQ=255)                                 gTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgcg                                                                                                       >  2:210779/1‑140 (MQ=255)                                    atcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAg                                                                                                    >  1:919643/1‑140 (MQ=255)                                           ggCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGcc                                                                                             >  2:119147/1‑140 (MQ=255)                                            gCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCa                                                                                            >  1:286138/1‑140 (MQ=255)                                                     ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCac                                                                                   <  1:548612/140‑1 (MQ=255)                                                       cgccgtCGATGTTCCGCGGTGCCAGTCCGTCGTCCTGTCTGCCGTCGCATTTTGCGTGCAAGGGCTGAGAGGCATGATCGCGACGTATACCGCTCGGGCAGGGAACCCCCTGATCGCGCAGGTTCGCCAGCTGATCacga                                                                                 <  2:348306/135‑1 (MQ=255)                                                       cGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCacga                                                                                 >  1:407794/1‑140 (MQ=255)                                                        gACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCacgac                                                                                <  2:791559/140‑1 (MQ=255)                                                                   tCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGt                                                                     >  2:73307/1‑140 (MQ=255)                                                                      gcgcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCa                                                                  <  2:453892/140‑1 (MQ=255)                                                                       cgcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCAt                                                                 >  1:620721/1‑140 (MQ=255)                                                                          ttCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGt                                                              <  1:669313/140‑1 (MQ=255)                                                                          ttCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGt                                                              >  1:746608/1‑140 (MQ=255)                                                                          ttCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGt                                                              >  2:669313/1‑140 (MQ=255)                                                                            ccAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTa                                                            >  1:147068/1‑140 (MQ=255)                                                                            ccAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTa                                                            >  2:505556/1‑140 (MQ=255)                                                                            ccAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTa                                                            >  2:524352/1‑140 (MQ=255)                                                                            ccAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTa                                                            >  2:683012/1‑140 (MQ=255)                                                                                ttcttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGAt                                                        <  2:620721/140‑1 (MQ=255)                                                                                 tcttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATc                                                       <  2:919643/140‑1 (MQ=255)                                                                                     cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTc                                                                   >  1:648905/1‑124 (MQ=255)                                                                                     cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTc                                                                   <  2:648905/124‑1 (MQ=255)                                                                                        tctcTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTAcc                                                >  2:553404/1‑140 (MQ=255)                                                                                              tGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCa                                          >  1:573800/1‑140 (MQ=255)                                                                                               gCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAg                                         <  2:286138/140‑1 (MQ=255)                                                                                                 cGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAgcg                                       <  2:247589/140‑1 (MQ=255)                                                                                                 cGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAgcg                                       >  1:620691/1‑140 (MQ=255)                                                                                                  gTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAgcgc                                      <  2:407794/140‑1 (MQ=255)                                                                                                   tCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGcc                                     <  1:495573/140‑1 (MQ=255)                                                                                                          cTGCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCg                              <  1:688290/140‑1 (MQ=255)                                                                                                            gCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCgtg                            <  2:243720/140‑1 (MQ=255)                                                                                                            gCGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCgtg                            <  2:147068/140‑1 (MQ=255)                                                                                                             cGTGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCgtgt                           >  2:753150/1‑140 (MQ=255)                                                                                                               tGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGtgttg                         >  1:915966/1‑140 (MQ=255)                                                                                                               tGCAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGtgttg                         <  1:683012/140‑1 (MQ=255)                                                                                                                 cAATTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTgttgtt                       <  1:524352/140‑1 (MQ=255)                                                                                                                   aTTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCa                     <  1:210779/140‑1 (MQ=255)                                                                                                                   aTTGGTCAGAGGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCa                     <  1:73307/140‑1 (MQ=255)                                                                                                                          agagGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGt              <  2:620691/140‑1 (MQ=255)                                                                                                                            agGTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTg            >  1:55300/1‑140 (MQ=255)                                                                                                                              gTATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGt          <  2:746608/140‑1 (MQ=255)                                                                                                                               tATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGtt         <  1:553404/140‑1 (MQ=255)                                                                                                                               tATGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGtt         <  1:467136/140‑1 (MQ=255)                                                                                                                                aTGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTc        >  2:943955/1‑140 (MQ=255)                                                                                                                                 tGATCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTcc       >  1:241548/1‑140 (MQ=255)                                                                                                                                    tCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCa    <  2:55300/140‑1 (MQ=255)                                                                                                                                    tCGCGGCGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCa    >  2:729934/1‑140 (MQ=255)                                                                                                                                      gcggcgGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATa  >  1:417299/1‑140 (MQ=255)                                                                                                                                      gcggcgGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATa  >  1:230117/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         TAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATA  >  NC_000913/3034916‑3035188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑