Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,517,080	T→G	D76A (GAT→GCT)	damX ←	cell division protein DamX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,517,080	0	T	G	100.0%	216.3 / NA	57	D76A (GAT→GCT)	damX	cell division protein DamX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/30);  total (27/30)

AACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGT  >  NC_000913/3516943‑3517213                                                                                                                                          |                                                                                                                                      aaCCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCATGGTTTCAGCt                                                                                                                                     >  1:925622/1‑140 (MQ=255)           gatgatCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGGTTCGt                                                                                                                           >  2:1036476/1‑140 (MQ=255)           gatgatCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGt                                                                                                                           >  2:50137/1‑140 (MQ=255)             tgatgaACAGCAGGAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAACGGGTTTACTGGCTGCTTTTTTGCGATTACGCGGACGACGCTCTACGCGCTCTTCATCGACGGTTTCAGCTTCGGAttactca                                                                                                                         >  2:578555/1‑135 (MQ=255)             tgatCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCa                                                                                                                         >  2:804314/1‑140 (MQ=255)              gatCAACAGCAGAAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCaa                                                                                                                        >  2:403349/1‑140 (MQ=255)                      gcagTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATttcttct                                                                                                                >  2:88856/1‑140 (MQ=255)                       cagTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATttcttctt                                                                                                               >  1:987235/1‑140 (MQ=255)                         gTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCg                                                                                                             >  2:149803/1‑140 (MQ=255)                          tAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGa                                                                                                            >  1:72628/1‑140 (MQ=255)                            gAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGAtt                                                                                                          <  2:408172/140‑1 (MQ=255)                              aCCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTc                                                                                                        <  2:593001/140‑1 (MQ=255)                                cAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCg                                                                                                      <  1:88856/140‑1 (MQ=255)                                cAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCg                                                                                                      <  1:50137/140‑1 (MQ=255)                                  gAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGt                                                                                                    >  2:639049/1‑140 (MQ=255)                                   aaTGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGtt                                                                                                   <  1:804314/140‑1 (MQ=255)                                     tGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTcc                                                                                                 <  1:810057/140‑1 (MQ=255)                                                 tcatcatATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGctct                                                                                     <  2:72628/140‑1 (MQ=255)                                                   atcatATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACCCGCTCTTCATCCATGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCttt                                                                                   <  2:925622/140‑1 (MQ=255)                                                     catATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCttttt                                                                                 <  1:794403/140‑1 (MQ=255)                                                     catATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCttttt                                                                                 <  1:724289/140‑1 (MQ=255)                                                        ataCTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTgcg                                                                              >  2:142913/1‑140 (MQ=255)                                                        ataCTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTgcg                                                                              <  1:925312/140‑1 (MQ=255)                                                            tGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACg                                                                          >  2:689312/1‑140 (MQ=255)                                                            tGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACg                                                                          >  2:256810/1‑140 (MQ=255)                                                             gACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGct                                                                                       >  2:877328/1‑126 (MQ=255)                                                             gACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGct                                                                                       <  1:877328/126‑1 (MQ=255)                                                              aCGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAg                                                                        >  1:786323/1‑140 (MQ=255)                                                              aCGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAg                                                                        >  1:1045838/1‑140 (MQ=255)                                                                   aaGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGa                                                                   >  1:339678/1‑140 (MQ=255)                                                                        ggTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTTCGCCCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCg                                                              <  2:786323/140‑1 (MQ=255)                                                                        ggTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCg                                                              <  1:479918/140‑1 (MQ=255)                                                                             aCTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGt                                                         <  1:403349/140‑1 (MQ=255)                                                                             aCTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGt                                                         >  2:174730/1‑140 (MQ=255)                                                                             aCTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGt                                                         >  2:585619/1‑140 (MQ=255)                                                                               tGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCa                                                       <  2:339678/140‑1 (MQ=255)                                                                                  ctgctTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAgtg                                                    <  1:149803/140‑1 (MQ=255)                                                                                   tgctTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAgtgt                                                   >  2:505521/1‑140 (MQ=255)                                                                                   tgctTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAgtgt                                                   >  2:820991/1‑140 (MQ=255)                                                                                       tttttGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGtcatc                                               <  2:77027/140‑1 (MQ=255)                                                                                       tttttGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGtcatc                                               <  2:987235/140‑1 (MQ=255)                                                                                       tttttGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGtcatc                                               <  1:423594/140‑1 (MQ=255)                                                                                       tttttGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGtcatc                                               <  1:224593/140‑1 (MQ=255)                                                                                                ttACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAat                                      <  1:258024/140‑1 (MQ=255)                                                                                                            cgCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAAtt                          >  1:493868/1‑140 (MQ=255)                                                                                                                    gcgcTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCg                  <  1:505521/140‑1 (MQ=255)                                                                                                                      gcTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGt                <  1:820991/140‑1 (MQ=255)                                                                                                                        tctTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTc              >  2:82297/1‑140 (MQ=255)                                                                                                                         ctTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCg             >  2:296017/1‑140 (MQ=255)                                                                                                                           tCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGcc           <  1:374562/140‑1 (MQ=255)                                                                                                                             aTCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCAc         <  1:689312/140‑1 (MQ=255)                                                                                                                             aTCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCAc         <  1:256810/140‑1 (MQ=255)                                                                                                                              tCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACg        <  1:585619/140‑1 (MQ=255)                                                                                                                               ccACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGt       <  1:1040395/140‑1 (MQ=255)                                                                                                                               ccACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGt       >  1:220498/1‑140 (MQ=255)                                                                                                                               ccACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCACACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGt       <  1:1036476/140‑1 (MQ=255)                                                                                                                                  cGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCa    >  2:943246/1‑140 (MQ=255)                                                                                                                                  cGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCa    >  1:909909/1‑140 (MQ=255)                                                                                                                                    gTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGt  <  1:876101/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      AACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGT  >  NC_000913/3516943‑3517213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑