Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,071,509	C→A	G86V (GGC→GTC)	rutD ←	putative aminoacrylate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,071,509	0	C	A	100.0%	188.8 / NA	54	G86V (GGC→GTC)	rutD	putative aminoacrylate hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/24);  total (30/24)

TAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACT  >  NC_000913/1071373‑1071640                                                                                                                                         |                                                                                                                                    tAATCGTTCGCGATCCTGAAAACAGCGGAGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCTCCAGCGCACCGAGCGCATGGACGa                                                                                                                                  <  1:1045957/140‑1 (MQ=255) tAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGa                                                                                                                                  >  1:1046709/1‑140 (MQ=255) tAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGa                                                                                                                                  <  1:794275/140‑1 (MQ=255) tAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGa                                                                                                                                  >  2:841356/1‑140 (MQ=255) tAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGa                                                                                                                                  >  1:322050/1‑140 (MQ=255)   aTCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGAcc                                                                                                                                >  2:46098/1‑140 (MQ=255)      gTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACt                                                                                                                             >  1:916984/1‑140 (MQ=255)      gTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACt                                                                                                                             >  2:486128/1‑140 (MQ=255)         cgcgAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCg                                                                                                                          >  1:424055/1‑140 (MQ=255)           cgAACCTGAGAAGAGCGTCGCGTATGGGCCTTTATTGTTAGCGAGCCGTTAACCCTGATCAGCACAGTTGCCGACGCGGGATAATCCAGCGCGAGCTGCATTCCCACCAGCGCACCGAGCGCCTGCACGACCACTGCGTa                                                                                                                        <  2:281540/140‑1 (MQ=255)           cgAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACt                                                                                                                             <  1:177904/135‑1 (MQ=255)           cgAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACt                                                                                                                             >  2:177904/1‑135 (MQ=255)                          cggcgCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTaa                                                                                                                       <  2:936219/126‑1 (MQ=255)                          cggcgCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTaa                                                                                                                       >  1:936219/1‑126 (MQ=255)                          cggcgCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGc                                                                                                         >  1:59582/1‑140 (MQ=255)                          cggcgCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGc                                                                                                         >  2:143284/1‑140 (MQ=255)                            gcgcGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCgg                                                                                                       <  1:486128/140‑1 (MQ=255)                               cgTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATcc                                                                                                             <  2:606798/131‑1 (MQ=255)                               cgTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATcc                                                                                                             >  1:606798/1‑131 (MQ=255)                                 tATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTAcc                                                                                                  <  1:841356/140‑1 (MQ=255)                                       cGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGcc                                                                                            <  1:143284/140‑1 (MQ=255)                                          ttATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGa                                                                                         >  1:720905/1‑140 (MQ=255)                                          ttATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGa                                                                                         >  2:724414/1‑140 (MQ=255)                                            aTTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATg                                                                                       >  2:589418/1‑140 (MQ=255)                                            aTTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATg                                                                                       <  2:322050/140‑1 (MQ=255)                                               cGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCa                                                                                                 >  2:1018328/1‑127 (MQ=255)                                               cGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCa                                                                                                 <  1:1018328/127‑1 (MQ=255)                                                                gCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTggg                                                                   <  2:859984/140‑1 (MQ=255)                                                                   gATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGa                                                                <  2:762262/140‑1 (MQ=255)                                                                   gATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGa                                                                <  2:424055/140‑1 (MQ=255)                                                                    aTCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGAt                                                               >  1:484773/1‑140 (MQ=255)                                                                           caGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTaa                                                        <  1:724414/140‑1 (MQ=255)                                                                              ttACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTg                                                           >  2:629730/1‑134 (MQ=255)                                                                              ttACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTg                                                           <  1:629730/134‑1 (MQ=255)                                                                              ttACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAAtct                                                     >  1:42937/1‑140 (MQ=255)                                                                                aCCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAAtcttc                                                   >  2:509462/1‑140 (MQ=255)                                                                                       cgGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGc                                            >  1:115666/1‑140 (MQ=255)                                                                                             aaTCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCg                                      <  1:589418/140‑1 (MQ=255)                                                                                             aaTCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCg                                      <  1:46098/140‑1 (MQ=255)                                                                                               tCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCggg                                    >  2:737908/1‑140 (MQ=255)                                                                                                    cgcCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGattat                               >  2:813837/1‑140 (MQ=255)                                                                                                    cgcCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGattat                               >  2:1059688/1‑140 (MQ=255)                                                                                                    cgcCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGattat                               >  1:153051/1‑140 (MQ=255)                                                                                                       cAGCTGCATTCCCACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGc                            <  2:916984/140‑1 (MQ=255)                                                                                                                 cccGCCAGCGCACCGGGCGCATGGACGGCCACTGCGTAATGCTCACGCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCcgcg                  <  2:338184/140‑1 (MQ=255)                                                                                                                 cccACCAGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCcgcg                  <  2:59582/140‑1 (MQ=255)                                                                                                                   caccaGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCt                <  2:42937/140‑1 (MQ=255)                                                                                                                     ccaGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTgg              >  2:539078/1‑140 (MQ=255)                                                                                                                     ccaGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTgg              >  2:125119/1‑140 (MQ=255)                                                                                                                       aGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTc            >  1:205208/1‑140 (MQ=255)                                                                                                                       aGCGCACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTc            >  1:941294/1‑140 (MQ=255)                                                                                                                        gcgcACCGATCGCATGGTCGTCCACTGCGTAATGCTCCATCCCTGCGGCTATCAGCGCGTGATGCAGTTCCGCTGCCGTCTGGGCGATACTGTAATCTTCTGCCAGCGTGTGGGGAGTATTGCCGGCGCCGCGCTGGTCg           >  2:613970/1‑140 (MQ=255)                                                                                                                         cgcACCGAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGt          >  2:970418/1‑140 (MQ=255)                                                                                                                               gAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGa    <  1:330984/140‑1 (MQ=255)                                                                                                                               gAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGa    <  2:484773/140‑1 (MQ=255)                                                                                                                               gAGCGCATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGa    <  1:539078/140‑1 (MQ=255)                                                                                                                                 gcgcATGGACGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACt  <  1:125119/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACT  >  NC_000913/1071373‑1071640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑