Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,516	G→T	V148V (GTC→GTA)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,516	0	G	T	100.0%	328.5 / NA	92	V148V (GTC→GTA)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/51);  total (41/51)

TGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCG  >  NC_000913/7380‑7649                                                                                                                                         |                                                                                                                                      tGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACg                                                                                                                                    >  2:1710679/1‑140 (MQ=255)    ttGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCg                                                                                                                                 <  1:553093/140‑1 (MQ=255)         agcagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAg                                                                                                                            <  1:81000/140‑1 (MQ=255)         agcagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAg                                                                                                                            <  2:791164/140‑1 (MQ=255)         agcagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAg                                                                                                                            <  1:409087/140‑1 (MQ=255)         agcagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAg                                                                                                                            <  2:1293812/140‑1 (MQ=255)          gcagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAg                                                                                                                            >  1:962611/1‑139 (MQ=255)          gcagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAg                                                                                                                            <  2:962611/139‑1 (MQ=255)           cagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGaa                                                                                                                          <  1:1323656/140‑1 (MQ=255)              agTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGc                                                                                                                       >  2:813979/1‑140 (MQ=255)                   aGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATc                                                                                                                  <  2:305314/140‑1 (MQ=255)                       aGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgc                                                                                                              >  2:1183168/1‑140 (MQ=255)                        gCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             >  1:44853/1‑140 (MQ=255)                        gCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             <  2:1207710/140‑1 (MQ=255)                        gCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             >  2:1181078/1‑140 (MQ=255)                        gCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             >  2:19629/1‑140 (MQ=255)                        gCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             >  2:268920/1‑140 (MQ=255)                        gCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             >  2:1099196/1‑140 (MQ=255)                        gCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             <  2:237892/140‑1 (MQ=255)                        gCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             <  1:980270/140‑1 (MQ=255)                        gCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             <  1:1457980/140‑1 (MQ=255)                        gCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             >  2:824501/1‑140 (MQ=255)                        gCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             >  1:1207710/1‑140 (MQ=255)                        gCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             >  1:911170/1‑140 (MQ=255)                         cGAGAATAATGCCTGTCACCAGCGTGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcgc                                                                                                            >  1:110697/1‑140 (MQ=255)                         cGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             >  1:213100/1‑139 (MQ=255)                         cGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             <  2:213100/139‑1 (MQ=255)                          gagaATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCa                                                                                                           >  1:48611/1‑140 (MQ=255)                              ataatGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATccc                                                                                                       >  1:192468/1‑140 (MQ=255)                              ataatGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATccc                                                                                                       <  2:20226/140‑1 (MQ=255)                                 atGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             >  2:1381290/1‑131 (MQ=255)                                 atGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAgcg                                                                                                             <  1:1381290/131‑1 (MQ=255)                                  tGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGc                                                                                                   >  1:947409/1‑140 (MQ=255)                                      tGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgc                                                                                               <  1:824501/140‑1 (MQ=255)                                       gTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                              <  1:1505648/140‑1 (MQ=255)                                       gTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                              <  2:155942/140‑1 (MQ=255)                                       gTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                              <  2:1685225/140‑1 (MQ=255)                                       gTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                              <  2:1050687/140‑1 (MQ=255)                                       gTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                              <  2:185004/140‑1 (MQ=255)                                       gTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                              >  2:967753/1‑140 (MQ=255)                                       gTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                              <  1:502676/140‑1 (MQ=255)                                       gTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                              <  1:1270196/140‑1 (MQ=255)                                       gTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                              <  1:1181078/140‑1 (MQ=255)                                        tCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcgc                                                                                             <  2:203132/140‑1 (MQ=255)                                        tCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcgc                                                                                             <  2:947409/140‑1 (MQ=255)                                        tCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcgc                                                                                             <  2:453456/140‑1 (MQ=255)                                         cACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcgcg                                                                                            <  1:890796/140‑1 (MQ=255)                                         cACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcgcg                                                                                            <  1:1262898/140‑1 (MQ=255)                                         cACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                              >  1:858988/1‑138 (MQ=255)                                         cACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                              <  2:858988/138‑1 (MQ=255)                                           ccAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGcc                                                                                          <  1:813979/140‑1 (MQ=255)                                            cAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCa                                                                                         >  2:907963/1‑140 (MQ=255)                                                  gggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATAcc                                                                                   >  1:988148/1‑140 (MQ=255)                                                  gggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATAcc                                                                                   >  2:968188/1‑140 (MQ=255)                                                  gggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATAcc                                                                                   >  2:535709/1‑140 (MQ=255)                                                    gAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                              >  1:1697618/1‑127 (MQ=255)                                                    gAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                              <  2:1697618/127‑1 (MQ=255)                                                         tCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcgcg                                                                                            <  2:736691/124‑1 (MQ=255)                                                         tCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcgcg                                                                                            >  1:736691/1‑124 (MQ=255)                                                           aaaaGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTc                                                                          <  1:1457467/140‑1 (MQ=255)                                                                aaaaaCTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCa                                                                     <  2:1583969/140‑1 (MQ=255)                                                                     cTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGaaa                                                                <  1:535709/140‑1 (MQ=255)                                                                     cTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGaaa                                                                <  1:968188/140‑1 (MQ=255)                                                                     cTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGaaa                                                                <  2:192468/140‑1 (MQ=255)                                                                      tCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAAc                                                               <  1:1183168/140‑1 (MQ=255)                                                                          ggCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGcc                                                           <  1:967753/140‑1 (MQ=255)                                                                              cgGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCAtt                                                       >  1:58224/1‑140 (MQ=255)                                                                               gggCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTg                                                      <  2:206678/140‑1 (MQ=255)                                                                               gggCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTg                                                      <  2:300681/140‑1 (MQ=255)                                                                               gggCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTg                                                      >  2:1359356/1‑140 (MQ=255)                                                                                ggCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGaaa                                                                >  1:1704642/1‑129 (MQ=255)                                                                                ggCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGaaa                                                                <  2:1704642/129‑1 (MQ=255)                                                                                         gTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGtt                                            <  1:19629/140‑1 (MQ=255)                                                                                         gTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGtt                                            <  2:48611/140‑1 (MQ=255)                                                                                         gTTCGCTTGAACTCCGCTGAAAATAATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGTGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGtt                                            <  1:63222/140‑1 (MQ=255)                                                                                                 gAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATaa                                    <  2:44853/140‑1 (MQ=255)                                                                                                  aaCTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATaaa                                   >  2:1350043/1‑140 (MQ=255)                                                                                                      ccGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtg                               <  2:792271/140‑1 (MQ=255)                                                                                                       cGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtgt                              >  1:190660/1‑140 (MQ=255)                                                                                                       cGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtgt                              >  1:157911/1‑140 (MQ=255)                                                                                                       cGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtgt                              >  1:1766142/1‑140 (MQ=255)                                                                                                       cGCTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtgt                              >  1:983569/1‑140 (MQ=255)                                                                                                         cTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGc                            >  2:1752596/1‑140 (MQ=255)                                                                                                         cTGAAAATTATGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGc                            >  2:960691/1‑140 (MQ=255)                                                                                                                  aTGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACa                   <  1:1099196/140‑1 (MQ=255)                                                                                                                  aTGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACa                   <  2:988148/140‑1 (MQ=255)                                                                                                                   tGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACAt                  >  1:191416/1‑140 (MQ=255)                                                                                                                   tGCCATAGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACAt                  >  2:1700862/1‑140 (MQ=255)                                                                                                                         aGGCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCa            >  1:663036/1‑140 (MQ=255)                                                                                                                          ggCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCaa           >  1:286107/1‑140 (MQ=255)                                                                                                                                   cAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCg  <  2:630491/140‑1 (MQ=255)                                                                                                                                   cAAAAATACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCg  >  1:630491/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      TGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCG  >  NC_000913/7380‑7649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑