Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,498,314	G→A	G163R (GGG→AGG)	cysG →	siroheme synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,498,314	0	G	A	100.0%	293.5 / NA	82	G163R (GGG→AGG)	cysG	siroheme synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/41);  total (41/41)

GACCGCTCACCGCTCATGGTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCGGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAAC  >  NC_000913/3498179‑3498443                                                                                                                                        |                                                                                                                                  gACCGCTCACCGCTCATGGTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCg                                                                                                                               <  2:1418724/140‑1 (MQ=255)     gctcaccgctcaTGGTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTg                                                                                                                           >  1:591378/1‑140 (MQ=255)      ctcaccgctcaTGGTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGa                                                                                                                          >  1:234494/1‑140 (MQ=255)      ctcaccgctcaTGGTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGa                                                                                                                          >  2:1272967/1‑140 (MQ=255)      ctcaccgctcaTGGTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGa                                                                                                                          >  2:1199081/1‑140 (MQ=255)      ctcaccgctcaTGGTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACG‑CGGGCAATTACGCAGGCGAGTGaa                                                                                                                         >  2:609964/1‑140 (MQ=255)            gctcaTGGTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAaacaac                                                                                                                    >  2:1506167/1‑140 (MQ=255)            gctcaTGGTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAaacaac                                                                                                                    >  1:1306155/1‑140 (MQ=255)             ctcaTGGTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAacaaca                                                                                                                   >  2:57423/1‑140 (MQ=255)             ctcaTGGTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAacaaca                                                                                                                   <  2:1167721/140‑1 (MQ=255)              tcaTGGTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGTAATTACGCAGGCGAGTGAAACAACAg                                                                                                                  >  2:53115/1‑140 (MQ=255)                 tGGTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGTAATTACGCAGGCGAGTGAAACAACAGTTc                                                                                                               <  1:53115/140‑1 (MQ=255)                   gTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGc                                                                                                             <  2:1663242/140‑1 (MQ=255)                   gTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGc                                                                                                             <  1:615668/140‑1 (MQ=255)                     aGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCa                                                                                                           <  1:1522317/140‑1 (MQ=255)                       cGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACg                                                                                                         <  1:1199081/140‑1 (MQ=255)                          tctcCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGcc                                                                                                            >  1:1410694/1‑134 (MQ=255)                          tctcCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGcc                                                                                                            <  2:1410694/134‑1 (MQ=255)                          tctcCTCTGGCAGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATg                                                                                                      <  1:593141/140‑1 (MQ=255)                            tcctcTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATggg                                                                                                    >  2:1103412/1‑140 (MQ=255)                            tcctcTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATggg                                                                                                    >  1:525457/1‑140 (MQ=255)                             cctcTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGt                                                                                                   <  2:1194747/140‑1 (MQ=255)                                ctGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAg                                                                                                <  1:57423/140‑1 (MQ=255)                                 tGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGc                                                                                               <  1:1225425/140‑1 (MQ=255)                                   gcggcACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGt                                                                                             >  2:553728/1‑140 (MQ=255)                                        aCCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCg                                                                                        >  2:31303/1‑140 (MQ=255)                                          ctctCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGtt                                                                                      >  1:1089378/1‑140 (MQ=255)                                             tcCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCt                                                                                   <  1:538197/140‑1 (MQ=255)                                               cGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTgg                                                                                 <  1:1272967/140‑1 (MQ=255)                                                    cTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGaa                                                                            >  2:214926/1‑140 (MQ=255)                                                       gCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTggg                                                                                >  2:654953/1‑133 (MQ=255)                                                       gCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTggg                                                                                <  1:654953/133‑1 (MQ=255)                                                       gCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGgag                                                                              <  1:1358591/135‑1 (MQ=255)                                                       gCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGgag                                                                              >  2:1358591/1‑135 (MQ=255)                                                       gCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAAtt                                                                         >  1:1598053/1‑140 (MQ=255)                                                       gCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAAtt                                                                         >  1:816872/1‑140 (MQ=255)                                                       gCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAAtt                                                                         >  1:87253/1‑140 (MQ=255)                                                         aCGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGt                                                                       <  1:1103412/140‑1 (MQ=255)                                                         aCGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGt                                                                       <  2:1507784/140‑1 (MQ=255)                                                           gCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTc                                                                     >  1:88617/1‑140 (MQ=255)                                                            ccTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCg                                                                    >  2:792191/1‑140 (MQ=255)                                                            ccTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCg                                                                    >  1:288074/1‑140 (MQ=255)                                                             cTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGt                                                                   <  1:1506167/140‑1 (MQ=255)                                                                 tGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAAc                                                               >  1:463551/1‑140 (MQ=255)                                                                   cgcgAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGa                                                             <  2:105621/140‑1 (MQ=255)                                                                   cgcgAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGa                                                             <  1:267529/140‑1 (MQ=255)                                                                   cgcgAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCAAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGa                                                             <  2:5893/140‑1 (MQ=255)                                                                     cgAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGAcc                                                           >  2:1100574/1‑140 (MQ=255)                                                                       aaaaaCTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGtt                                                                  >  1:1648753/1‑131 (MQ=255)                                                                       aaaaaCTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGtt                                                                  <  2:1648753/131‑1 (MQ=255)                                                                       aaaaaCTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGc                                                         <  2:816872/140‑1 (MQ=255)                                                                                  tcgctgctgCCGTTACATCTGGGCCAGGTAGCGAAATACG‑CGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCg                                             <  1:609964/137‑1 (MQ=255)                                                                                   cACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCg                                             <  1:402089/140‑1 (MQ=255)                                                                                   cACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCg                                             <  2:1692324/140‑1 (MQ=255)                                                                                      tgctgcCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTg                                          >  1:684175/1‑140 (MQ=255)                                                                                      tgctgcCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTg                                          >  2:556351/1‑140 (MQ=255)                                                                                           ccGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCaaa                                     <  1:1100574/140‑1 (MQ=255)                                                                                           ccGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCaaa                                     <  2:395701/140‑1 (MQ=255)                                                                                             gTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAaaca                                   >  2:929382/1‑140 (MQ=255)                                                                                                      gggCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAaacaa                                  >  2:1472024/1‑132 (MQ=255)                                                                                                      gggCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAaacaa                                  <  1:1472024/132‑1 (MQ=255)                                                                                                       ggCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGaa                         >  2:4804/1‑140 (MQ=255)                                                                                                       ggCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGaa                         <  1:929382/140‑1 (MQ=255)                                                                                                        gCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGaaa                        >  1:37770/1‑140 (MQ=255)                                                                                                        gCCAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGaaa                        >  1:1780264/1‑140 (MQ=255)                                                                                                          cAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGc                      <  2:1306155/140‑1 (MQ=255)                                                                                                          cAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGc                      <  2:87253/140‑1 (MQ=255)                                                                                                          cAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGc                      <  1:4804/140‑1 (MQ=255)                                                                                                          cAGGTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTAGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGc                      <  2:234494/140‑1 (MQ=255)                                                                                                            ggTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGc                      >  1:851484/1‑138 (MQ=255)                                                                                                            ggTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGc                      <  2:851484/138‑1 (MQ=255)                                                                                                            ggTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCa                    >  2:247716/1‑140 (MQ=255)                                                                                                             gTAGCGAAATACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCAt                   >  2:974166/1‑140 (MQ=255)                                                                                                                   aaaTACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGa             <  2:288074/140‑1 (MQ=255)                                                                                                                      tACGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAAc          >  1:1765612/1‑140 (MQ=255)                                                                                                                       aCGCCGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACg         >  1:1585715/1‑140 (MQ=255)                                                                                                                           cGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCg     <  1:332079/140‑1 (MQ=255)                                                                                                                           cGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCg     >  1:1695583/1‑140 (MQ=255)                                                                                                                           cGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCg     <  2:684175/140‑1 (MQ=255)                                                                                                                           cGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCg     <  1:556351/140‑1 (MQ=255)                                                                                                                           cGGGCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCg     <  2:525457/140‑1 (MQ=255)                                                                                                                              gCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAAc  >  2:648328/1‑140 (MQ=255)                                                                                                                              gCAATTACGCAGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAAc  >  2:278502/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                  GACCGCTCACCGCTCATGGTAGCGGTCTCCTCTGGCGGCACCTCTCCGGTTCTGGCACGCCTGTTGCGCGAAAAACTTGAATCACTGCTGCCGTTACATCTGGGCCAGGTAGCGAAATACGCCGGGCAATTACGCGGGCGAGTGAAACAACAGTTCGCCACGATGGGTGAGCGTCGCCGTTTCTGGGAGAAATTGTTCGTTAACGACCGCCTGGCGCAGTCGCTGGCAAACAACGATCAGAAAGCCATTACTGAAACGACCGAAC  >  NC_000913/3498179‑3498443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑