Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	136,712	T→C	D69G (GAC→GGC)	yacC ←	putative lipoprotein YacC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	136,712	0	T	C	100.0%	304.5 / NA	80	D69G (GAC→GGC)	yacC	putative lipoprotein YacC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/39);  total (41/39)

GACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGTCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCTTCGGCTTCAGACTCGCT  >  NC_000913/136576‑136848                                                                                                                                         |                                                                                                                                         gACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGcccc                                                                                                                                       <  1:652750/140‑1 (MQ=255)       ggCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGt                                                                                                                                 >  1:1536006/1‑140 (MQ=255)        gCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGGGTCGTAATTACTGAGGCCCCACTGGtt                                                                                                                                >  2:16145/1‑140 (MQ=255)                gCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAg                                                                                                                        <  2:1536006/140‑1 (MQ=255)                gCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAg                                                                                                                        >  2:422228/1‑140 (MQ=255)                gCTTAAGGAATCGCGCTCCCGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTGCTGAGGCCCCACTGGTTTTGCTGAg                                                                                                                        <  1:1765090/140‑1 (MQ=255)                  ttAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCg                                                                                                                      >  2:1712545/1‑140 (MQ=255)                  ttAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCg                                                                                                                      >  2:1718820/1‑140 (MQ=255)                          tCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCg                                                                                                                      <  1:298758/132‑1 (MQ=255)                          tCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCg                                                                                                                      >  2:298758/1‑132 (MQ=255)                          tCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGAc                                                                                                              >  2:1762920/1‑140 (MQ=255)                          tCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGAc                                                                                                              >  2:372533/1‑140 (MQ=255)                             cgGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAg                                                                                                           <  2:959406/140‑1 (MQ=255)                                    gggCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGcga                                                                                                    >  2:1522635/1‑140 (MQ=255)                                    gggCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGcga                                                                                                    <  2:673477/140‑1 (MQ=255)                                     ggCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGcgac                                                                                                   >  1:1531491/1‑140 (MQ=255)                                     ggCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGcgac                                                                                                   <  2:631422/140‑1 (MQ=255)                                          tGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAAtt                                                                                              >  1:379378/1‑140 (MQ=255)                                          tGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAAtt                                                                                              >  2:1215680/1‑140 (MQ=255)                                          tGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAAtt                                                                                              >  2:1284320/1‑140 (MQ=255)                                          tGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAAtt                                                                                              >  1:535989/1‑140 (MQ=255)                                          tGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAAtt                                                                                              >  1:1527492/1‑140 (MQ=255)                                            cATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTg                                                                                            <  1:349847/140‑1 (MQ=255)                                              ttttttAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGcc                                                                                          <  1:1712545/140‑1 (MQ=255)                                              ttttttAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGcc                                                                                          <  2:343656/140‑1 (MQ=255)                                                 tttAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGt                                                                                       <  2:1628457/140‑1 (MQ=255)                                                 tttAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGt                                                                                       <  2:1531491/140‑1 (MQ=255)                                                 tttAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGt                                                                                       <  2:379378/140‑1 (MQ=255)                                                      cGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGt                                                                                  <  1:1522635/140‑1 (MQ=255)                                                      cGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGt                                                                                  <  1:1762920/140‑1 (MQ=255)                                                      cGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGt                                                                                  <  1:721958/140‑1 (MQ=255)                                                              atgccaatgccGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTg                                                                          <  2:535989/140‑1 (MQ=255)                                                              atgccaatgccGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTg                                                                          <  1:422228/140‑1 (MQ=255)                                                              atgccaatgccGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTg                                                                          <  2:77704/140‑1 (MQ=255)                                                                    atgccGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGt                                                                                  >  1:162237/1‑126 (MQ=255)                                                                    atgccGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGt                                                                                  <  2:162237/126‑1 (MQ=255)                                                                    atgccGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAAcc                                                                    >  2:1050237/1‑140 (MQ=255)                                                                          cTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATc                                                              >  2:457273/1‑140 (MQ=255)                                                                          cTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATc                                                              >  1:1542026/1‑140 (MQ=255)                                                                          cTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATc                                                              >  1:236105/1‑140 (MQ=255)                                                                           tGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCg                                                             <  1:836914/140‑1 (MQ=255)                                                                           tGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCg                                                             <  2:1542026/140‑1 (MQ=255)                                                                           tGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCg                                                             <  1:372533/140‑1 (MQ=255)                                                                           tGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCACCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCg                                                             <  1:1749859/140‑1 (MQ=255)                                                                            gagaTCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGt                                                            >  1:616375/1‑140 (MQ=255)                                                                                tCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGt                                                                        <  2:1018140/124‑1 (MQ=255)                                                                                tCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGt                                                                        >  1:1018140/1‑124 (MQ=255)                                                                                         cTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGAc                                               >  2:287210/1‑140 (MQ=255)                                                                                         cTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGAc                                               >  2:809629/1‑140 (MQ=255)                                                                                         cTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGAc                                               <  1:1420153/140‑1 (MQ=255)                                                                                            tCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACaaa                                            >  2:408322/1‑140 (MQ=255)                                                                                                cACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACt                                        >  1:1585471/1‑140 (MQ=255)                                                                                                  ccGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGc                                      >  1:356373/1‑140 (MQ=255)                                                                                                      gggCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGtt                                  >  2:1463983/1‑140 (MQ=255)                                                                                                      gggCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGtt                                  >  1:1684175/1‑140 (MQ=255)                                                                                                      gggCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGtt                                  >  2:1018045/1‑140 (MQ=255)                                                                                                       ggCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTa                                 >  2:1696450/1‑140 (MQ=255)                                                                                                        gCTTTCATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTaa                                >  2:731490/1‑140 (MQ=255)                                                                                                             cATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAAt                              <  1:427321/137‑1 (MQ=255)                                                                                                             cATGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAAt                              >  2:427321/1‑137 (MQ=255)                                                                                                               tGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGcc                         <  2:616375/140‑1 (MQ=255)                                                                                                               tGTCGAAGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGcc                         <  2:758979/140‑1 (MQ=255)                                                                                                                   gAAGGTGTCGTAATTGCTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCatat                     >  2:1159585/1‑140 (MQ=255)                                                                                                                    aaGGTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATc                    >  2:641780/1‑140 (MQ=255)                                                                                                                      ggTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAGGACAAAAACTGCCGTTAAATCGGCCATATCtt                  <  2:1684175/140‑1 (MQ=255)                                                                                                                      ggTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGTCATATCtt                  >  1:1203138/1‑140 (MQ=255)                                                                                                                      ggTGTCGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCtt                  <  1:1156871/140‑1 (MQ=255)                                                                                                                      ggTGTCGTAATGACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCTTTAGGTAAGTGCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCtt                  >  2:1022629/1‑140 (MQ=255)                                                                                                                           cGTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCTTCGGCt             >  1:445316/1‑140 (MQ=255)                                                                                                                            gTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGCCCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAACCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCTTCGGCtt            <  1:1309139/140‑1 (MQ=255)                                                                                                                            gTAATTACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCTTCGGCtt            <  1:1337165/140‑1 (MQ=255)                                                                                                                                ttACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCTTCGGCTTCAGa        <  1:809629/140‑1 (MQ=255)                                                                                                                                 tACTGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCTTCGGCTTCAGAc       >  2:773683/1‑140 (MQ=255)                                                                                                                                    tGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCTGCCATATCtt                  <  1:1588380/126‑1 (MQ=255)                                                                                                                                    tGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCTGCCATATCtt                  >  2:1588380/1‑126 (MQ=255)                                                                                                                                    tGAGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCTTCGGCTTCAGACTCg    <  2:751660/140‑1 (MQ=255)                                                                                                                                      aGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCTTCGGCtt            <  2:192886/130‑1 (MQ=255)                                                                                                                                      aGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCTTCGGCtt            >  1:192886/1‑130 (MQ=255)                                                                                                                                      aGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCTTCGGCTTCAGACTCGCt  <  2:605549/140‑1 (MQ=255)                                                                                                                                      aGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCTTCGGCTTCAGACTCGCt  <  1:731490/140‑1 (MQ=255)                                                                                                                                      aGGCCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCTTCGGCTTCAGACTCGCt  <  1:408322/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTCATGTCGAAGGTGTCGTAATTACTGAGGTCCCACTGGTTTTGCTGAGCGAAAAAGACCAGTGCGCGACGAATTTGCCCGTTAGGTAAGTTCTGGTAACCACAATCGTTCTTCAGAAAGACAAAAACTGCCGTTAAATCGGCCATATCTTCGGCTTCAGACTCGCT  >  NC_000913/136576‑136848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑