| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,614,496 | 0 | C | T | 20.0% | 97.9 / 14.6 | 40 | intergenic (+236/+184) | yjjV/yjjW | putative DNase YjjV/putative glycyl‑radical enzyme activating enzyme YjjW |
| Reads supporting (aligned to +/- strand): ref base C (17/15); new base T (5/3); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACG > NC_000913/4614359‑4614620 | tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCgg > 1:1074027/1‑140 (MQ=255)tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCgg < 2:1074027/140‑1 (MQ=255)tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCgg < 1:799321/140‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAAt < 1:565339/140‑1 (MQ=255) aTCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGt < 1:1042712/140‑1 (MQ=255) ggCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTcgcg < 2:1045299/140‑1 (MQ=255) cacaATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACGCCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCagg > 1:254812/1‑138 (MQ=11) cacaATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACGCCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCagg > 1:468428/1‑138 (MQ=11) cacaATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGc > 2:972828/1‑140 (MQ=255) acaATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGcc > 2:706013/1‑140 (MQ=255) agtGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTa < 2:439545/138‑1 (MQ=255) cTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGt < 1:706013/140‑1 (MQ=255) tGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTc < 2:1015856/122‑1 (MQ=255) tGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTc > 1:1015856/1‑122 (MQ=255) aTGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTtg > 1:509700/1‑140 (MQ=255) gCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCtgtg > 2:173016/1‑140 (MQ=255) gACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGcc > 1:663530/1‑140 (MQ=255) aCGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGcct > 1:439545/1‑139 (MQ=255) tGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCg < 2:509700/140‑1 (MQ=255) gTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGcc > 2:500288/1‑140 (MQ=255) ccTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGt < 2:125592/140‑1 (MQ=255) cTACAAGTCTGTGCCGACCCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGtt < 1:486086/140‑1 (MQ=255) cTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGtt > 2:231660/1‑140 (MQ=255) aCAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTcg > 1:126270/1‑140 (MQ=255) cAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAg < 2:366009/132‑1 (MQ=255) cAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAg > 1:366009/1‑132 (MQ=255) aaGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTcacg > 2:1005045/1‑140 (MQ=255) gtttgtgCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGcgcc < 1:1102142/137‑1 (MQ=2) tttgtgCCGAACCGTAGGAGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTcgcg < 1:697028/138‑1 (MQ=14) tgCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATc > 1:109180/1‑140 (MQ=255) ccGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCg > 2:1155587/1‑140 (MQ=255) ccGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCg > 2:134203/1‑140 (MQ=255) cGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCgg > 2:767432/1‑140 (MQ=255) aaCCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCa > 2:1102142/1‑140 (MQ=17) aaCCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCa > 2:94811/1‑140 (MQ=255) aCCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAg < 1:1155587/140‑1 (MQ=255) t‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCAACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTgg < 2:730967/140‑1 (MQ=16) t‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTgg > 1:820369/1‑140 (MQ=255) cACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCg < 1:94811/140‑1 (MQ=255) g‑ccgcATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACg < 1:500288/140‑1 (MQ=255) | TAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGT‑‑‑AGGCCGGATAAGGCGTTCACG‑CCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACG > NC_000913/4614359‑4614620 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |