Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,240,9850TG26.5% 96.3 / 14.4 49S348A (TCT→GCT) sstTserine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (10/26);  new base G (13/0);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 4.36e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGAC  >  NC_000913/3240846‑3241119
                                                                                                                                           |                                                                                                                                       
gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGt                                                                                                                                         <  1:290709/140‑1 (MQ=255)
   cgcAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGtctc                                                                                                                                      <  1:499205/140‑1 (MQ=255)
          aTCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTctgctgct                                                                                                                               <  1:566959/140‑1 (MQ=255)
            cACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGa                                                                                                                             <  2:346042/140‑1 (MQ=255)
                  tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCAc                                                                                                                       <  1:187249/140‑1 (MQ=255)
                    ccGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTg                                                                                                                     >  1:531520/1‑140 (MQ=255)
                     cGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                                                                    >  1:786109/1‑140 (MQ=255)
                      gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                                   <  1:1045492/140‑1 (MQ=255)
                      gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                                   <  1:929425/140‑1 (MQ=255)
                       tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGcc                                                                                                                  >  1:128005/1‑140 (MQ=255)
                       tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                                   <  1:245873/139‑1 (MQ=255)
                       tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGcc                                                                                                                  >  1:632551/1‑140 (MQ=255)
                       tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGc                                                                                                                   >  2:245873/1‑139 (MQ=255)
                         ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTctgct                                                                                                                                  >  1:500236/1‑122 (MQ=255)
                         ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTctgct                                                                                                                                  <  2:500236/122‑1 (MQ=255)
                         ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                                   <  2:936110/137‑1 (MQ=255)
                         ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGc                                                                                                                   >  1:936110/1‑137 (MQ=255)
                         ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTg                                                                                                                >  2:589316/1‑140 (MQ=255)
                           gACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                                                                              <  2:128005/140‑1 (MQ=255)
                               ctggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                                   <  2:648405/131‑1 (MQ=255)
                               ctggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGc                                                                                                                   >  1:648405/1‑131 (MQ=255)
                                 ggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACCGGCCCGTAATATGt                                                                                                        >  1:918713/1‑140 (MQ=255)
                                      cGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGt                                                                                                   <  1:998631/140‑1 (MQ=255)
                                            aTACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCg                                                                                             >  2:269174/1‑140 (MQ=255)
                                              aCGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                                                           <  2:1091389/140‑1 (MQ=255)
                                              aCGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                                                           <  1:1212788/140‑1 (MQ=255)
                                              aCGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                                                           <  2:531520/140‑1 (MQ=255)
                                                 cTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                                        <  2:632551/140‑1 (MQ=255)
                                                 cTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                                        >  1:472462/1‑140 (MQ=255)
                                                   gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGata                                                                                      >  1:1019211/1‑140 (MQ=255)
                                                   gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGata                                                                                      >  2:231427/1‑140 (MQ=255)
                                                   gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTTCTGCTGATCCCCCCTGCCTGTAATATGTTCGGTATTTTGAACGaat                                                                                      >  2:8062/1‑138 (MQ=255)
                                                              tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                                                                           >  2:540864/1‑140 (MQ=255)
                                                              tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCCCTGGCCTGTAATATGTTTGGTATTTTGAACGATATCGCCATGCAg                                                                           >  2:999163/1‑140 (MQ=255)
                                                              tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                                                                           >  2:879275/1‑140 (MQ=255)
                                                                        cACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCg                                                                <  2:786109/140‑1 (MQ=255)
                                                                          cGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTc                                                              <  1:999163/140‑1 (MQ=255)
                                                                          cGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGAT‑CCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCg                                                             >  1:836330/1‑140 (MQ=255)
                                                                           ggCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCg                                                             >  2:554653/1‑140 (MQ=255)
                                                                            gcgcTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGGATTTCGAACGATATCGCCATGCAGGG‑GGGTGCCGTCgg                                                            >  2:213374/1‑140 (MQ=255)
                                                                                     ttGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATTCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTatcat                                                   <  1:282862/140‑1 (MQ=255)
                                                                                       gAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCCGTAATATGTTCGGTATTTCGGACGATATCGCCATGCAGGG‑GGTTGCCGTCGGCTTTatcatcc                                                 >  2:212410/1‑139 (MQ=255)
                                                                                        aGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTATCATCgg                                                <  1:1234631/140‑1 (MQ=255)
                                                                                          cGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTATCATCGGCg                                              <  1:8062/140‑1 (MQ=255)
                                                                                            tggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGTCTTCTGCTGATTCCCCCGGCCTGTAATATGTTTGGTATTTCGGAAGATATCGCCATTCGGGT‑GGCTGCCCTCGGCTTTTTCATCCGCGTa                                            >  2:275578/1‑140 (MQ=255)
                                                                                            tggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACAATATCGCCCTGCAGGT‑GGTTGCCGTCGGCTTTATCATCGGCGTa                                            >  1:612658/1‑140 (MQ=255)
                                                                                                     ctctGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGAc                                   <  1:212410/140‑1 (MQ=255)
                                                                                                        tgtgtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCCCTGGCCTGTAATATGTTCGGTATTTTGGACGATATTGCCATGCAGGC‑GGGTGGCGGCGGCTTTATCATCCGCGGCTTGCAGGGctct                                >  2:139901/1‑140 (MQ=255)
                                                                                                           gtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGc                             >  1:1136681/1‑140 (MQ=255)
                                                                                                             gCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGa                           >  1:1062254/1‑140 (MQ=255)
                                                                                                               cTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGaaa                         <  1:231427/140‑1 (MQ=255)
                                                                                                                  tgGCGCATCCGGCGTGGCGGGGGGGTCTCTGCCGCTTAATCCACTGGGCCGTAATATGTTTGGTGTTTTGAACGATATCGCCATGCAGGTGGGTTGCC‑TCGGCTTTATCATCTGCGCATTTGAGGACTCCTGCGaaaacc                      >  2:1202655/1‑137 (MQ=255)
                                                                                                                  tgGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGGATTTTGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACcg                      >  2:173090/1‑140 (MQ=255)
                                                                                                                    gcgcATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACcgcg                    >  1:472397/1‑140 (MQ=255)
                                                                                                                         tCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGaa               <  1:540864/140‑1 (MQ=255)
                                                                                                                         tCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGaa               <  1:554653/140‑1 (MQ=255)
                                                                                                                                    gggggggTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTg    <  1:275578/140‑1 (MQ=255)
                                                                                                                                      gggggTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGAc  <  2:1019211/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
GGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGT‑GGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGAC  >  NC_000913/3240846‑3241119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑