Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	224,814	A→G	noncoding (1044/1542 nt)	rrsH →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	224,814	0	A	G	100.0%	213.9 / NA	56	noncoding (1044/1542 nt)	rrsH	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/25);  total (31/25)

AACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGA  >  NC_000913/224678‑224949                                                                                                                                         |                                                                                                                                        aaCTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAg                                                                                                                                      <  1:1029329/140‑1 (MQ=255)  aCTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAgg                                                                                                                                     <  1:502145/140‑1 (MQ=255)      aaaTGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGtgct                                                                                                                                 >  1:156627/1‑140 (MQ=255)       aaTGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGtgctg                                                                                                                                >  2:501499/1‑140 (MQ=255)         tGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCa                                                                                                                              <  1:245632/140‑1 (MQ=255)           aaTTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATg                                                                                                                            <  2:1058756/140‑1 (MQ=255)            aTTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATgg                                                                                                                           <  2:679944/140‑1 (MQ=255)             ttGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAATGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGGGGCAGGTGCTGCATGGc                                                                                                                          >  2:443565/1‑140 (MQ=255)                    gggCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTgtcgtc                                                                                                                   <  2:412310/140‑1 (MQ=255)                                gCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTc                                                                                                              <  1:442850/133‑1 (MQ=255)                                gCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTc                                                                                                              >  2:442850/1‑133 (MQ=255)                                 cGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTtgtg                                                                                                      <  2:753247/140‑1 (MQ=255)                                      gAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATg                                                                                                 <  2:280149/140‑1 (MQ=255)                                          aTGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTgg                                                                                             >  2:453053/1‑140 (MQ=255)                                           tgtgGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGtgttgt                                                                                                       <  1:1195674/129‑1 (MQ=255)                                           tgtgGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGtgttgt                                                                                                       >  2:1195674/1‑129 (MQ=255)                                                          tGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACg                                                                             >  2:416027/1‑140 (MQ=255)                                                              aCGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAgcg                                                                         <  1:794909/140‑1 (MQ=255)                                                               cgcgAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAgcgc                                                                        <  1:501499/140‑1 (MQ=255)                                                               cgcgAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAgcgc                                                                        >  1:144204/1‑140 (MQ=255)                                                                         cTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATc                                                              >  1:741535/1‑140 (MQ=255)                                                                           tACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCtt                                                            >  1:187333/1‑140 (MQ=255)                                                                            aCCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCttt                                                           >  2:383153/1‑140 (MQ=255)                                                                             ccTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGGGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCtttt                                                          <  1:443565/140‑1 (MQ=255)                                                                               tGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTttgt                                                        <  2:156627/140‑1 (MQ=255)                                                                                ggTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTttgtt                                                       >  2:755814/1‑140 (MQ=255)                                                                                ggTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTttgtt                                                       >  2:1148481/1‑140 (MQ=255)                                                                                  tCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGc                                                     >  2:954352/1‑140 (MQ=255)                                                                                  tCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGc                                                     >  2:1050738/1‑140 (MQ=255)                                                                                   cTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGcc                                                    >  1:686996/1‑140 (MQ=255)                                                                                     tGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAg                                                  <  2:144204/140‑1 (MQ=255)                                                                                     tGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAg                                                  <  1:1050738/140‑1 (MQ=255)                                                                                      gACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGc                                                 >  1:1001808/1‑140 (MQ=255)                                                                                      gACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGc                                                 >  1:1072602/1‑140 (MQ=255)                                                                                      gACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGc                                                 >  2:780425/1‑140 (MQ=255)                                                                                      gACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGc                                                 >  1:1127830/1‑140 (MQ=255)                                                                                           ccACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTcc                                            >  1:968591/1‑140 (MQ=255)                                                                                           ccACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTcc                                            >  1:722591/1‑140 (MQ=255)                                                                                              caGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTccggc                                         >  2:1079805/1‑140 (MQ=255)                                                                                              caGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTccggc                                         <  1:780425/140‑1 (MQ=255)                                                                                              caGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTccggc                                         >  2:931746/1‑140 (MQ=255)                                                                                              caGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTccggc                                         <  1:383153/140‑1 (MQ=255)                                                                                                 aaCTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTccggccgg                                      <  1:954352/140‑1 (MQ=255)                                                                                                     ttCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAAc                                  <  1:453053/140‑1 (MQ=255)                                                                                                      tCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACt                                 >  1:1124078/1‑140 (MQ=255)                                                                                                      tCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCAAGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTccg                                           <  2:140545/130‑1 (MQ=255)                                                                                                      tCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCAAGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTccg                                           >  1:140545/1‑130 (MQ=255)                                                                                                                gATTGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGAc                       >  1:387742/1‑140 (MQ=255)                                                                                                                   tGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGc                    <  2:1001808/140‑1 (MQ=37)                                                                                                                   tGGTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGc                    <  1:755814/140‑1 (MQ=37)                                                                                                                    ggTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGcc                   <  2:968591/140‑1 (MQ=25)                                                                                                                     gTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCa                  >  2:1029669/1‑140 (MQ=25)                                                                                                                     gTGCCTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCa                  >  2:909031/1‑140 (MQ=25)                                                                                                                        ccTTCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTg               >  2:110349/1‑140 (MQ=21)                                                                                                                           tCGGGAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATa            >  1:603473/1‑140 (MQ=25)                                                                                                                             gggAACTGTGAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATaaa          <  2:603473/140‑1 (MQ=21)                                                                                                                                     tgAGGCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTggagga  >  2:376826/1‑140 (MQ=17)                                                                                                                                         |                                                                                                                                        AACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGA  >  NC_000913/224678‑224949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑