Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,370,757	A→T	D247V (GAT→GTT)	arnD →	putative 4‑deoxy‑4‑formamido‑L‑arabinose‑phosphoundecaprenol deformylase ArnD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,370,757	0	A	T	100.0%	143.5 / NA	41	D247V (GAT→GTT)	arnD	putative 4‑deoxy‑4‑formamido‑L‑arabinose‑phosphoundecaprenol deformylase ArnD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (27/14);  total (27/14)

AAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCA  >  NC_000913/2370622‑2370886                                                                                                                                        |                                                                                                                                  aaGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTtctc                                                                                                                               >  1:797779/1‑140 (MQ=255)      aTTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCaa                                                                                                                          >  2:220031/1‑140 (MQ=255)      aTTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCaa                                                                                                                          >  2:10659/1‑140 (MQ=255)           tCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAAcgcg                                                                                                                     <  1:1239636/140‑1 (MQ=255)           tCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAAcgcg                                                                                                                     <  2:347544/140‑1 (MQ=255)           tCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAAcgcg                                                                                                                     >  2:502648/1‑140 (MQ=255)           tCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAAcgcg                                                                                                                     <  1:10659/140‑1 (MQ=255)                aTGTGAAAGGAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCt                                                                                                                >  2:1141704/1‑140 (MQ=255)                aTGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAAcgcg                                                                                                                     <  2:538798/135‑1 (MQ=255)                aTGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAAcgcg                                                                                                                     >  1:538798/1‑135 (MQ=255)                         cAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGc                                                                                                       >  2:123834/1‑140 (MQ=255)                            aagaTTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAAcgcg                                                                                                                     <  2:478481/123‑1 (MQ=255)                            aagaTTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAAcgcg                                                                                                                     >  1:478481/1‑123 (MQ=255)                                       ggttggttACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGccc                                                                                         <  1:220031/140‑1 (MQ=255)                                              tACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGc                                                                                  <  1:63236/140‑1 (MQ=255)                                              tACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGc                                                                                  <  1:871904/140‑1 (MQ=255)                                               aCTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCg                                                                                 <  1:394533/140‑1 (MQ=255)                                                cTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGa                                                                                <  1:502648/140‑1 (MQ=255)                                                cTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGa                                                                                <  2:797779/140‑1 (MQ=255)                                                   aaCCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACt                                                                             >  1:735770/1‑140 (MQ=255)                                                       gCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTa                                                                         >  1:929920/1‑140 (MQ=255)                                                             tGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCagag                                                                   >  1:480315/1‑140 (MQ=255)                                                                 agaTAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGt                                                               >  2:132889/1‑140 (MQ=255)                                                                   aTAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTg                                                             >  1:534136/1‑140 (MQ=255)                                                                             gCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGAc                                                   >  2:551195/1‑140 (MQ=255)                                                                                     ataCCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAgttgtt                                           <  2:735770/140‑1 (MQ=255)                                                                                           ttCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGa                                     >  2:524438/1‑140 (MQ=255)                                                                                           ttCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGa                                     >  1:950688/1‑140 (MQ=255)                                                                                           ttCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGa                                     >  2:609210/1‑140 (MQ=255)                                                                                           ttCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGa                                     >  2:1237302/1‑140 (MQ=255)                                                                                            tCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGaa                                    >  1:189102/1‑140 (MQ=255)                                                                                                   gAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGc                             <  2:534136/140‑1 (MQ=255)                                                                                                      gTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCgg                          >  1:465719/1‑140 (MQ=255)                                                                                                         gAAGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACg                       >  1:485556/1‑140 (MQ=255)                                                                                                           aGGCTGCGCTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTg                     <  1:123834/140‑1 (MQ=255)                                                                                                                 cgcTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAggct               >  2:416447/1‑140 (MQ=255)                                                                                                                 cgcTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAggct               >  1:674336/1‑140 (MQ=255)                                                                                                                 cgcTTATCAGCATAATTTTGTGGTTCTCCTCAAACGCGCAGCTAAGGAAGGCGTGACATTTTGCCCTTTCAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAggct               >  2:579419/1‑140 (MQ=255)                                                                                                                           cATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTg     >  2:507744/1‑140 (MQ=255)                                                                                                                           cATAATTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTg     >  1:382102/1‑140 (MQ=255)                                                                                                                              aaTTTTGTGGTTCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCca  >  2:376372/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                  AAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCA  >  NC_000913/2370622‑2370886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑