Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	594,664	A→C	I36S (ATC→AGC)	cusS ←	sensory histidine kinase CusS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	594,664	0	A	C	100.0%	255.2 / NA	64	I36S (ATC→AGC)	cusS	sensory histidine kinase CusS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/28);  total (36/28)

ACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCACCTCAAGCATGTAACCCACGCC  >  NC_000913/594528‑594798                                                                                                                                         |                                                                                                                                       aCTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCa                                                                                                                                     <  1:1179046/140‑1 (MQ=255)  cTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCAt                                                                                                                                    <  2:1164717/140‑1 (MQ=255)   tGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATg                                                                                                                                   >  2:882539/1‑140 (MQ=255)   tGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATg                                                                                                                                   >  2:763528/1‑140 (MQ=255)    gACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGa                                                                                                                                  >  2:709619/1‑140 (MQ=255)    gACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGa                                                                                                                                  >  1:30143/1‑140 (MQ=255)     aCGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGAt                                                                                                                                 <  1:763528/140‑1 (MQ=255)                     tCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGaaa                                                                                                                 >  2:502926/1‑140 (MQ=255)                       aTTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGaaaaa                                                                                                               >  1:777982/1‑140 (MQ=255)                       aTTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGaaaaa                                                                                                               >  1:83817/1‑140 (MQ=255)                       aTTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGaaaaa                                                                                                               <  1:95744/140‑1 (MQ=255)                            gCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAAcgccg                                                                                                          <  2:83817/140‑1 (MQ=255)                                cGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGAt                                                                                                      >  1:278887/1‑140 (MQ=255)                                cGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGAt                                                                                                      >  1:452382/1‑140 (MQ=255)                                       gCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGcc                                                                                               <  2:1002228/140‑1 (MQ=255)                                         gTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAg                                                                                             <  1:502926/140‑1 (MQ=255)                                          tttCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAgg                                                                                            <  2:278887/140‑1 (MQ=255)                                          tttCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAgg                                                                                            <  2:452382/140‑1 (MQ=255)                                          tttCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAgg                                                                                            <  2:777982/140‑1 (MQ=255)                                           ttCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGc                                                                                           <  2:592315/140‑1 (MQ=255)                                            tCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCt                                                                                          >  1:580631/1‑140 (MQ=255)                                            tCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCt                                                                                          >  1:143009/1‑140 (MQ=255)                                                  gggTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCt                                                                                          >  2:1018758/1‑134 (MQ=255)                                                  gggTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCt                                                                                          <  1:1018758/134‑1 (MQ=255)                                                      gATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAg                                                                                <  1:882539/140‑1 (MQ=255)                                                        tttAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGt                                                                              <  1:709619/140‑1 (MQ=255)                                                                ccGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCggg                                                                      <  2:30143/140‑1 (MQ=255)                                                                  gTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGtt                                                                    >  1:107233/1‑140 (MQ=255)                                                                             gcgcTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGaaaa                                                         >  2:999989/1‑140 (MQ=255)                                                                             gcgcTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTAATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGaaaa                                                         >  1:2799/1‑140 (MQ=255)                                                                              cgcTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGc                                                                  >  2:872473/1‑130 (MQ=255)                                                                              cgcTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGc                                                                  <  1:872473/130‑1 (MQ=255)                                                                                          ttAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAAt                                            >  2:262325/1‑140 (MQ=255)                                                                                                cATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTa                                      >  2:735838/1‑140 (MQ=255)                                                                                                  ttAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACt                                    <  2:1090864/140‑1 (MQ=255)                                                                                                    aaTATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGCTAATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGa                                  <  2:2799/140‑1 (MQ=255)                                                                                                           tGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCg                           >  1:902559/1‑140 (MQ=255)                                                                                                           tGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCg                           >  1:71382/1‑140 (MQ=255)                                                                                                           tGCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCg                           >  1:634801/1‑140 (MQ=255)                                                                                                            gCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCgg                          >  2:152102/1‑140 (MQ=255)                                                                                                            gCTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCgg                          >  2:649022/1‑140 (MQ=255)                                                                                                             cTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCg                           <  2:1233894/138‑1 (MQ=255)                                                                                                             cTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCg                           >  1:1233894/1‑138 (MQ=255)                                                                                                             cTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGc                         >  2:1459/1‑140 (MQ=255)                                                                                                             cTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGc                         >  1:102624/1‑140 (MQ=255)                                                                                                             cTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGc                         >  2:740510/1‑140 (MQ=255)                                                                                                             cTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGc                         >  2:286244/1‑140 (MQ=255)                                                                                                             cTCGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGc                         >  2:304188/1‑140 (MQ=255)                                                                                                             cTCGGCAAAATGAACTTTTACCGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGc                         >  1:786077/1‑140 (MQ=255)                                                                                                               cGGCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGTAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCAc                       >  1:1013449/1‑140 (MQ=255)                                                                                                                ggCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAACCGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCAcc                      >  2:555882/1‑140 (MQ=255)                                                                                                                ggCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCAcc                      >  1:1096583/1‑140 (MQ=255)                                                                                                                ggCAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCAcc                      >  2:229290/1‑140 (MQ=255)                                                                                                                  cAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCgg                          >  1:782495/1‑134 (MQ=255)                                                                                                                  cAAAATGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCgg                          <  2:782495/134‑1 (MQ=255)                                                                                                                   aaaaTGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCACCTCa                   <  1:999989/140‑1 (MQ=255)                                                                                                                   aaaaTGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCACCTCa                   <  1:735838/140‑1 (MQ=255)                                                                                                                    aaaTGAACTTTTACTGAGTGGCTCATGATCCATGTAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCACCTCaa                  <  2:1013449/140‑1 (MQ=255)                                                                                                                    aaaTGAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCACCTCaa                  <  1:1459/140‑1 (MQ=255)                                                                                                                        gAACTTTTACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCACCTCAAGCAt              <  1:229290/140‑1 (MQ=255)                                                                                                                               tACTGAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCACCTCAAGCATGTAAccc       <  1:152102/140‑1 (MQ=255)                                                                                                                                   gAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCACCTCAAGCATGTAACCCAcgc   <  1:262325/140‑1 (MQ=255)                                                                                                                                   gAGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCACCTCAAGCATGTAACCCAcgc   <  2:902559/140‑1 (MQ=255)                                                                                                                                    aGTGGCTCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCACCTCAAGCATGTAACCCAcgcc  >  2:1226065/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGAAATGGCTTACTGACCATCCGGCACCTCAAGCATGTAACCCACGCC  >  NC_000913/594528‑594798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑