Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	302,390	Δ1 bp	coding (184/690 nt)	paoA ←	aldehyde dehydrogenase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	302,387	0	G	.	100.0%	221.3 / NA	51	coding (187/690 nt)	paoA	aldehyde dehydrogenase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (23/28);  total (23/28)

TACAGGCTCCGCACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCAGGGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGA  >  NC_000913/302252‑302499                                                                                                                                        |                                                                                                                 tACAGGCTCCGCACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCAgggca                                                                                                              >  1:850642/1‑138 (MQ=255) tACAGGCTCCGCACTGTCCGTGATCACAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCATCAGTGTCAg                                                                                                                  >  1:837641/1‑136 (MQ=255) tACAGGCTCCGCACTGTCCGTGATCACAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCATCAGTGTCAg                                                                                                                  <  2:837641/136‑1 (MQ=255)      gCTCCGCACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTAtc                                                                                                        >  1:183256/1‑140 (MQ=255)         ccGCACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATctct                                                                                                     >  2:1030912/1‑140 (MQ=255)         ccGCACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATctct                                                                                                     >  1:1184896/1‑140 (MQ=255)         ccGCACTGTACGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATctct                                                                                                     <  1:245062/140‑1 (MQ=255)           gCACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCAgggc                                                                                                               <  2:147681/129‑2 (MQ=255)           gCACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCAgggc                                                                                                               >  1:147681/1‑128 (MQ=255)            cgcTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCTCCTTCAGTGTCA‑GGGCATTATCTCTGgc                                                                                                  <  2:930344/138‑1 (MQ=255)            cACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGgc                                                                                                  <  1:729937/140‑1 (MQ=255)            cACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGgc                                                                                                  <  2:160032/140‑1 (MQ=255)            cACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGgc                                                                                                  <  2:657347/140‑1 (MQ=255)            cACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGgc                                                                                                  <  2:278003/140‑1 (MQ=255)             aCTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGgcg                                                                                                 <  1:1129066/140‑1 (MQ=255)               tGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGcgcg                                                                                               >  2:1024159/1‑140 (MQ=255)                 tCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCggg                                                                                             >  2:100548/1‑140 (MQ=255)                 tCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATACACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGgc                                                                                                  >  1:843236/1‑135 (MQ=255)                 tCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATACACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGgc                                                                                                  <  2:843236/135‑1 (MQ=255)                           cAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGcc                                                                                   >  2:415451/1‑140 (MQ=255)                           cAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGcc                                                                                   >  2:1097309/1‑140 (MQ=255)                           cAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGcc                                                                                   >  1:541019/1‑140 (MQ=255)                           cAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGcc                                                                                   >  2:1055849/1‑140 (MQ=255)                                  tCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGc                                                                            >  2:57936/1‑140 (MQ=255)                                        tACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTgccg                                                                      >  2:934300/1‑140 (MQ=255)                                         aCCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTgccgc                                                                     <  2:541019/140‑1 (MQ=255)                                         aCCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTgccgc                                                                     <  1:1055849/140‑1 (MQ=255)                                          ccGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTgccgcc                                                                    <  2:535191/140‑1 (MQ=255)                                            gATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAg                                                                  >  1:42682/1‑140 (MQ=255)                                             aTCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGc                                                                 >  1:472174/1‑140 (MQ=255)                                              tCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCg                                                                <  2:42682/140‑1 (MQ=255)                                               cAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGt                                                               <  2:472174/140‑1 (MQ=255)                                                   tGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGaa                                                           <  1:100548/140‑1 (MQ=255)                                                   tGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGaa                                                           <  2:104941/140‑1 (MQ=255)                                                    gCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAAt                                                          <  2:437153/140‑1 (MQ=255)                                                    gCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAAt                                                          <  1:57936/140‑1 (MQ=255)                                                    gCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAAt                                                          <  1:1024159/140‑1 (MQ=255)                                                                  aaaGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCAcg                                            >  1:995372/1‑140 (MQ=255)                                                                          cAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTcgc                                    <  2:1018090/140‑1 (MQ=255)                                                                          cAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTcgc                                    <  1:934300/140‑1 (MQ=255)                                                                                cGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTcgcccccgt                              >  1:894282/1‑135 (MQ=255)                                                                                cGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTcgcccccgt                              >  1:572220/1‑135 (MQ=255)                                                                                cGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGt                              >  2:1063722/1‑140 (MQ=255)                                                                                cGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGt                              >  1:592214/1‑140 (MQ=255)                                                                                  tGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTg                            <  2:592214/140‑1 (MQ=255)                                                                                  tGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTg                            <  1:1063722/140‑1 (MQ=255)                                                                                     ttCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTgcgg                         >  2:679289/1‑140 (MQ=255)                                                                                        gggTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGgcgc                      <  2:747851/140‑1 (MQ=255)                                                                                        gggTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGgcgc                      <  1:345375/140‑1 (MQ=255)                                                                                        gggTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGgcgc                      <  1:1097309/140‑1 (MQ=255)                                                                                        gggTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGgcgc                      <  1:1030912/140‑1 (MQ=255)                                                                                        gggTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGgcgc                      <  2:995372/140‑1 (MQ=255)                                                                                                 ccTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTaa             <  1:415451/140‑1 (MQ=255)                                                                                                  cTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCAGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCCGTTGCGGCGCTCACTTTTAt            >  1:430466/1‑140 (MQ=255)                                                                                                           ctCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGGCGCCGCCGTTGCGGGGCCCCCTTTTATCAAAAcgcc   >  2:456266/1‑139 (MQ=255)                                                                                                            tCGGTTTTGCCGTTCACCTTCAGTGTCA‑GGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGGCGCCGCCGTTGGGGGGCCCCCTTTTATCAGAAcgcgg  >  2:137632/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                 TACAGGCTCCGCACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCAAAGTGTCCAGTAGCGTGGTTCGGGTATCCACCTCAAGCTGCTCGGTTTTGCCGTTCACCTTCAGTGTCAGGGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGA  >  NC_000913/302252‑302499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑