Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT27.5% 71.2 / 19.8 40intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (1/28);  new base T (11/0);  total (12/28)
Fisher's exact test for biased strand distribution p-value = 5.19e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGA  >  NC_000913/3206298‑3206566
                                                                                                                                          |                                                                                                                                   
aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCa                                                                                                                                   <  1:1039309/140‑1 (MQ=255)
  aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGa                                                                                                                                 >  1:324875/1‑140 (MQ=255)
    cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACa                                                                                                                               <  2:1074448/140‑1 (MQ=255)
       tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCACACAAAt                                                                                                                            >  2:183057/1‑140 (MQ=255)
       tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACaaaa                                                                                                                            >  1:891339/1‑139 (MQ=255)
       tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGATaaaa                                                                                                                            >  1:674716/1‑139 (MQ=255)
         ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATAc                                                                                                                          <  2:708781/140‑1 (MQ=255)
          cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACa                                                                                                                         <  2:3923/140‑1 (MQ=255)
                 tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCTGAAAAAAAAAAAAACta                                                                                                                  >  1:306556/1‑139 (MQ=255)
                    tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                               <  2:716595/140‑1 (MQ=255)
                    tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                               <  1:931729/140‑1 (MQ=255)
                    tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                               <  1:783812/140‑1 (MQ=255)
                        gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTAGACAAAAAAAAAAAAAGGAGTTgg                                                                                                           >  2:570469/1‑139 (MQ=255)
                         cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAGAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGGTgtc                                                                                                          >  2:783812/1‑138 (MQ=255)
                             atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga                                                                                                      <  1:1165425/140‑1 (MQ=255)
                                ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGCCAttatga                                                                                                   >  2:775206/1‑140 (MQ=255)
                                 tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                  <  2:891339/140‑1 (MQ=255)
                                    ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                               <  1:1228053/140‑1 (MQ=255)
                                    ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                               <  2:490043/140‑1 (MQ=255)
                                        tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCCTGGTGAGCGgaaaa                                                                                           >  1:685239/1‑138 (MQ=255)
                                         aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGta                                                                                          <  2:54438/140‑1 (MQ=255)
                                           tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                        <  2:1055750/140‑1 (MQ=255)
                                              agacaggATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAg                                                                                     <  1:982621/134‑1 (MQ=255)
                                                     aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                              <  2:872048/140‑1 (MQ=255)
                                                               tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGGCATGATGAGCGGAAATAAAAAAAAAAAAGCAGTGAaaaa                                                                    >  2:174249/1‑140 (MQ=255)
                                                               tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑CATTTTTTCAGATAAAAACAAAAAATGGAGTGGCGCTGAAGAGGGAAAGAAAAAAAGAACAAGCAgcgtgttt                                                                    >  2:982621/1‑133 (MQ=255)
                                                                gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                   <  1:915061/140‑1 (MQ=255)
                                                                gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AATTTTTTCATACAAAAAAAAAAAATGGAGGTGGCATGATGAGCGGAAGAAAAAAAAAAAAAGCAGAGAATaaa                                                                   >  2:233988/1‑139 (MQ=255)
                                                                 ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGGGTTGCCATGATGAGCGAAAGTAATAAGCAACAAGCAGGGAaaaaat                                                                  >  1:1056916/1‑138 (MQ=255)
                                                                 ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGTTGGCATGATGAGCGGAAGAAATAAAAAACAAGCAGGGAaaaaaa                                                                  >  2:971449/1‑138 (MQ=255)
                                                                 ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGt                                                                  <  1:1003442/140‑1 (MQ=255)
                                                                  tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGtt                                                                 <  1:412118/140‑1 (MQ=255)
                                                                   aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTg                                                                <  1:775206/140‑1 (MQ=255)
                                                                          tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                         <  2:353639/140‑1 (MQ=255)
                                                                          tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                         <  1:233988/140‑1 (MQ=255)
                                                                           atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCAAACAAAAAAAAAAAAT‑GAGGTGGCCTGATGAGGGGAAATAAAAAACAAAAAGCAGAGAAAAAAATGACAgagaa                                                        >  2:762055/1‑139 (MQ=255)
                                                                           atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGACAAAAAAAAAAAAAGGGGGTGGCCTGATGAGGGAAAAGAATAAACAAAAAGCAGGGAAAAAAATTACAgagaa                                                        >  2:760201/1‑139 (MQ=255)
                                                                           atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGAGGTAGTTTGTAGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                        >  1:344180/1‑140 (MQ=255)
                                                                             tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCAACAAAAAAAAAAAAAGGAGTTTGCCTGATGAGGGAAAGTAATAAACAACAAGCAGTGAAAAAATTGACAGAGAttt                                                      >  2:1313/1‑139 (MQ=255)
                                                                                cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAATACAAAAAATTGGGGTGGCCTGATGAGGGGAAGAAATAAGCAAAAAGCAGTGAATAAATTGAAAGAGAATGTCg                                                   >  2:830140/1‑140 (MQ=255)
                                                                                 ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGc                                                  <  1:750104/140‑1 (MQ=255)
                                                                                  gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAAAAAAAAAAATTGAGGTGCCATGATGAGGGAAAGTAAAAAAAAAAAAGCAAGGAAAAAATTTACAGAGATTGTCGCt                                                 >  2:667641/1‑140 (MQ=255)
                                                                                     gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGTTGGCATGATGAGCGGAAATAATAAACAACAAGCAGGGAATAAATTGACAGAGATTGTCGCTAAc                                              >  2:444288/1‑140 (MQ=255)
                                                                                                   aCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt                                <  1:971449/140‑1 (MQ=255)
                                                                                                     cacaTTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGAAAAAAAAAAAAAATGGAGGTGGCATGATGAGGGGAAGTAAAAAG‑AAAAAGCAGTGAATAAAATGACAGAGAATGTCGGTAACTTTTACGCCATGAtttt                             >  2:849022/1‑139 (MQ=255)
                                                                                                            ttAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAAAGGGGGTGCCATGATGAGGGAAAGGAATAAAAAAAAAGCAGTGAATAAATTGACAGAGATTTTTGCTAACTTTACCGCCCTGATTTTTAcccc                       >  2:857243/1‑139 (MQ=255)
                                                                                                             tAGTGGTATTTTGGGGGGGTA‑AATTTTTCCAGAAAAAAAAAAAAACTGGAGTTGCCATGATGAGCAAAAGAAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGTTTGTCGTTAATTTTACCGCCATGATTTCTACCCGa                      <  2:612513/140‑1 (MQ=255)
                                                                                                             tAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                      <  1:174249/140‑1 (MQ=255)
                                                                                                              aGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                     <  1:570469/140‑1 (MQ=255)
                                                                                                                       ttGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg            <  1:444288/140‑1 (MQ=255)
                                                                                                                       ttGTGGCGGGA‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGAGGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg            <  1:1110053/140‑1 (MQ=255)
                                                                                                                          ggggggTG‑AATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCGGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACg         <  2:1056916/136‑1 (MQ=255)
                                                                                                                                 g‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa  <  1:1313/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                   
AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGA  >  NC_000913/3206298‑3206566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑