Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,704,723	G→A	T196I (ACC→ATC)	lepB ←	signal peptidase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,704,723	0	G	A	100.0%	196.3 / NA	56	T196I (ACC→ATC)	lepB	signal peptidase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/24);  total (32/24)

GCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGACCGGATCGTAAGTGACTTTATCGCCCGGT  >  NC_000913/2704599‑2704857                                                                                                                               |                                                                                                                                       gCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGc                                                                                                                         <  1:636354/140‑1 (MQ=255)  cGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCt                                                                                                                        >  1:542238/1‑140 (MQ=255)  cGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCt                                                                                                                        >  1:425827/1‑140 (MQ=255)    gTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGCGTGAGAAGATCTGAACGAAATCGCt                                                                                                                        <  2:309413/140‑1 (MQ=255)    gTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCg                                                                                                                      >  1:153690/1‑140 (MQ=255)    gTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCg                                                                                                                      >  1:422673/1‑140 (MQ=255)        gTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTc                                                                                                                  >  2:1225212/1‑140 (MQ=255)           acaTCACCCAGTGTCTCTTTATGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCAc                                                                                                               <  2:487408/140‑1 (MQ=255)               cACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGttt                                                                                                           <  2:350230/140‑1 (MQ=255)                   cAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGt                                                                                                       <  2:422673/140‑1 (MQ=255)                      tgtCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAgg                                                                                                    >  1:237714/1‑140 (MQ=255)                         ctctTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCGGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGGAGGTGa                                                                                                 >  2:738819/1‑140 (MQ=255)                         ctctTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTc                                                                                                                  <  1:393421/123‑1 (MQ=255)                         ctctTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTc                                                                                                                  >  2:393421/1‑123 (MQ=255)                           ctTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGAcc                                                                                               >  2:84430/1‑140 (MQ=255)                                cGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAg                                                                                          >  1:322114/1‑140 (MQ=255)                                cGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAg                                                                                          >  1:937045/1‑140 (MQ=255)                                  cTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAgcg                                                                                        >  1:69775/1‑140 (MQ=255)                                               aTTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGc                                                                           >  2:459405/1‑140 (MQ=255)                                               aTTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGc                                                                           <  2:194288/140‑1 (MQ=255)                                                     ttttCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCATGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGcc                                                                     >  1:325712/1‑140 (MQ=255)                                                      tttCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCg                                                                    <  2:237714/140‑1 (MQ=255)                                                          tttGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAAc                                                                >  1:1100035/1‑140 (MQ=255)                                                          tttGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAAc                                                                <  1:846123/140‑1 (MQ=255)                                                                  cGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATccc                                                        >  2:361669/1‑140 (MQ=255)                                                                             aCTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGt                                             <  1:1225212/140‑1 (MQ=255)                                                                             aCTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGt                                             <  2:937045/140‑1 (MQ=255)                                                                              cTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTc                                            >  1:267987/1‑140 (MQ=255)                                                                               ttCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCa                                           <  2:267987/140‑1 (MQ=255)                                                                               ttCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCa                                           <  2:390338/140‑1 (MQ=255)                                                                               ttCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCa                                           >  2:938401/1‑140 (MQ=255)                                                                                tCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAg                                          <  2:153690/140‑1 (MQ=255)                                                                                 cAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGc                                         <  2:322114/140‑1 (MQ=255)                                                                                 cAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGc                                         >  1:808299/1‑140 (MQ=255)                                                                                 cAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGc                                         >  1:1116119/1‑140 (MQ=255)                                                                                 cAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCATGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGc                                         <  2:325712/140‑1 (MQ=255)                                                                                  aaaGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGct                                        <  2:1100035/140‑1 (MQ=255)                                                                                        tCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTg                                  >  1:105949/1‑140 (MQ=255)                                                                                         ccGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTga                                 >  1:1054949/1‑140 (MQ=255)                                                                                         ccGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTga                                 >  2:817284/1‑140 (MQ=255)                                                                                         ccGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTga                                 <  1:459405/140‑1 (MQ=255)                                                                                         ccGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTga                                 >  1:613852/1‑140 (MQ=255)                                                                                           gCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTgaga                               >  2:412095/1‑140 (MQ=255)                                                                                            cTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGAc                              <  2:1116119/140‑1 (MQ=255)                                                                                            cTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGAc                              <  1:412095/140‑1 (MQ=255)                                                                                            cTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGAc                              >  2:627885/1‑140 (MQ=255)                                                                                            cTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGAc                              <  2:613852/140‑1 (MQ=255)                                                                                                tCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGACCGGa                          >  1:486311/1‑140 (MQ=255)                                                                                                tCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGACCGGa                          <  2:760505/140‑1 (MQ=255)                                                                                                tCGCTTCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGACCGGa                          >  1:1127040/1‑140 (MQ=255)                                                                                                     tCCCCACCATTACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGACCGGATCGTa                     <  1:817284/140‑1 (MQ=255)                                                                                                              ttACG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGACCGGATAGTAAGTGACttt            >  2:1026174/1‑140 (MQ=255)                                                                                                                aCG‑‑GCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGACCGGATCGTAAGTGACTTTAt          >  1:464356/1‑140 (MQ=255)                                                                                                                     gCGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGACCGGATCGTAAGTGACTTTATCGc       <  2:1107252/140‑1 (MQ=255)                                                                                                                      cGTGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGACCGGATCGTAAGTGACTTTATCGcc      >  2:524595/1‑140 (MQ=255)                                                                                                                        tGAGAAGATCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCAGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGACCGGATCGTAAGTGACTTTATCGCCCg    >  1:994463/1‑140 (MQ=255)                                                                                                                          agaagaTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGACCGGATCGTAAGTGACTTTATCGCCCGGt  >  2:248118/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                       GCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACG‑‑GCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCGGTTGAATCGTCAGCTCTTTTGAGACCGGATCGTAAGTGACTTTATCGCCCGGT  >  NC_000913/2704599‑2704857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑