Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,545,872	C→A	D83E (GAC→GAA)	nfuA →	iron‑sulfur cluster carrier protein NfuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,545,872	0	C	A	100.0%	319.9 / NA	91	D83E (GAC→GAA)	nfuA	iron‑sulfur cluster carrier protein NfuA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (40/51);  total (40/51)

AATGTGGCGTTTCTTATTGTCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGT  >  NC_000913/3545736‑3546007                                                                                                                                         |                                                                                                                                        aaTGTGGCGTTTCTTATTGTCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAg                                                                                                                                      >  2:995015/1‑140 (MQ=255)  aTGTGGCGTTTCTTATTGTCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGt                                                                                                                                     >  2:20860/1‑140 (MQ=255)     tgGCGTTTCTTATTGTCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTgg                                                                                                                                  <  1:197083/140‑1 (MQ=255)       gCGTTTCTTATTGTCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGt                                                                                                                                >  2:1204528/1‑140 (MQ=255)       gCGTTTCTTATTGTCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGt                                                                                                                                <  1:96197/140‑1 (MQ=255)           ttCTTATTGTCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTccc                                                                                                                            <  1:71971/140‑1 (MQ=255)             cTTATTGTCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAg                                                                                                                          >  1:291753/1‑140 (MQ=255)              ttATTGTCCGCCGGACGCTGTGGAAGCCACCGACACAGCCTTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGt                                                                                                                         <  1:362067/140‑1 (MQ=255)               tATTGTCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGtt                                                                                                                        <  1:995015/140‑1 (MQ=255)                  tGTCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAAc                                                                                                                     >  1:9404/1‑140 (MQ=255)                  tGTCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAAc                                                                                                                     <  2:225526/140‑1 (MQ=255)                     ccgccgGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCt                                                                                                                  >  2:139465/1‑140 (MQ=255)                      cgccgGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTg                                                                                                                 >  1:204306/1‑140 (MQ=255)                        ccgGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGaa                                                                                                               <  2:489568/140‑1 (MQ=255)                         cgGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGaaa                                                                                                              >  2:742717/1‑140 (MQ=255)                          ggACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCTATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAg                                                                                                             <  1:733666/140‑1 (MQ=255)                          ggACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAg                                                                                                             <  2:1149760/140‑1 (MQ=255)                          ggACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAg                                                                                                             <  2:801911/140‑1 (MQ=255)                          ggACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAg                                                                                                             <  1:865193/140‑1 (MQ=255)                          ggACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAg                                                                                                             <  2:317038/140‑1 (MQ=255)                              gCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGcccc                                                                                                         <  2:1131151/140‑1 (MQ=255)                              gCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGcccc                                                                                                         <  1:470419/140‑1 (MQ=255)                               cTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCg                                                                                                        <  1:586239/140‑1 (MQ=255)                                 gtgGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGaa                                                                                                      <  1:759244/140‑1 (MQ=255)                                     aaGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGcc                                                                                                  <  1:76693/140‑1 (MQ=255)                                        ccACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCaaa                                                                                               <  2:1050252/140‑1 (MQ=255)                                          aCCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAAt                                                                                             >  1:1066987/1‑140 (MQ=255)                                          aCCGACACAGCCATGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAAt                                                                                             >  2:895093/1‑140 (MQ=255)                                            cGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCaaatgcg                                                                                           >  1:707450/1‑136 (MQ=255)                                            cGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGc                                                                                           >  2:1094345/1‑140 (MQ=255)                                            cGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGc                                                                                           >  1:327327/1‑140 (MQ=255)                                              acacAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGcccc                                                                                                         <  2:37739/124‑1 (MQ=255)                                              acacAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGcccc                                                                                                         >  1:37739/1‑124 (MQ=255)                                               cacaGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTa                                                                                        <  2:1066987/140‑1 (MQ=255)                                                    cccTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTg                                                                                   >  1:1026265/1‑140 (MQ=255)                                                      cTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTaaa                                                                                      >  2:751815/1‑135 (MQ=255)                                                      cTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTaaa                                                                                      <  1:751815/135‑1 (MQ=255)                                                      cTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGc                                                                                 <  2:325445/140‑1 (MQ=255)                                                      cTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGc                                                                                 <  1:1204528/140‑1 (MQ=255)                                                        gAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAg                                                                               <  2:9404/140‑1 (MQ=255)                                                         aaaTTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGa                                                                              <  1:1094345/140‑1 (MQ=255)                                                         aaaTTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGa                                                                              >  2:710113/1‑140 (MQ=255)                                                             ttGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGAt                                                                          <  1:20860/140‑1 (MQ=255)                                                              tGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAATCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATg                                                                         >  2:770656/1‑140 (MQ=255)                                                              tGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATg                                                                         >  1:1045981/1‑140 (MQ=255)                                                              tGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATg                                                                         >  1:694716/1‑140 (MQ=255)                                                              tGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATg                                                                         >  2:937546/1‑140 (MQ=255)                                                               gACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGc                                                                        <  2:112778/140‑1 (MQ=255)                                                               gACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGc                                                                        <  2:291753/140‑1 (MQ=255)                                                                 ccTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCAc                                                                      <  1:256164/140‑1 (MQ=255)                                                                     ctgACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCt                                                                  >  1:517285/1‑140 (MQ=255)                                                                      tgACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTg                                                                 >  1:619814/1‑140 (MQ=255)                                                                               aTGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAgcgc                                                        <  1:433784/140‑1 (MQ=255)                                                                               aTGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAgcgc                                                        <  2:694716/140‑1 (MQ=255)                                                                               aTGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAgcgc                                                        >  2:519901/1‑140 (MQ=255)                                                                               aTGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAgcgc                                                        <  2:163767/140‑1 (MQ=255)                                                                                tGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGcgcg                                                       <  1:742717/140‑1 (MQ=255)                                                                                  ttGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTg                                                     <  2:769848/140‑1 (MQ=255)                                                                                  ttGATGAGTTAAGAGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTg                                                     >  2:469396/1‑140 (MQ=255)                                                                                   tgatgaGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTgg                                                    <  1:139465/140‑1 (MQ=255)                                                                                    gatgaGTTGAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGa                                                   >  1:1069727/1‑140 (MQ=255)                                                                                          ttAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTatat                                             >  2:568870/1‑140 (MQ=255)                                                                                            aaGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATAtgc                                           >  1:260158/1‑140 (MQ=255)                                                                                              gcgcACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATAtgctg                                         <  2:1026265/140‑1 (MQ=255)                                                                                                    cATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCg                                   <  2:1069727/140‑1 (MQ=255)                                                                                                    cATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCg                                   >  1:695189/1‑140 (MQ=255)                                                                                                    cATACCTGGAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCg                                   <  1:469396/140‑1 (MQ=255)                                                                                                           ggAAGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCa                            <  2:84694/140‑1 (MQ=255)                                                                                                             aaGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAAc                          <  2:695189/140‑1 (MQ=255)                                                                                                              aGATGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAAcc                         >  1:294837/1‑140 (MQ=255)                                                                                                                aTGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCca                       >  1:718416/1‑140 (MQ=255)                                                                                                                 tGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCcac                      >  2:1028057/1‑140 (MQ=255)                                                                                                                 tGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCcac                      >  1:525301/1‑140 (MQ=255)                                                                                                                 tGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCcac                      >  2:321704/1‑140 (MQ=255)                                                                                                                 tGCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCcac                      >  2:1193192/1‑140 (MQ=255)                                                                                                                  gCAGAGATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCcaca                     >  2:121120/1‑140 (MQ=255)                                                                                                                    agagATCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGc                   <  2:1045981/140‑1 (MQ=255)                                                                                                                      agaTCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATACACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCtt                 >  2:275662/1‑140 (MQ=255)                                                                                                                       gaTCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAATCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTg                <  1:770656/140‑1 (MQ=255)                                                                                                                       gaTCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTg                <  1:937546/140‑1 (MQ=255)                                                                                                                        aTCGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGc               <  2:525301/140‑1 (MQ=255)                                                                                                                          cGATTTTGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTg             <  1:519901/140‑1 (MQ=255)                                                                                                                              tttGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCa         <  1:710113/140‑1 (MQ=255)                                                                                                                              tttGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCa         <  1:1028057/140‑1 (MQ=255)                                                                                                                                tGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACg       >  2:934061/1‑140 (MQ=255)                                                                                                                                tGTTACCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATACACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACg       <  1:275662/140‑1 (MQ=255)                                                                                                                                    aCCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACggtgg   >  1:46601/1‑140 (MQ=255)                                                                                                                                    aCCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACggtgg   >  1:557708/1‑140 (MQ=255)                                                                                                                                    aCCGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACggtgg   <  1:1193192/140‑1 (MQ=255)                                                                                                                                     ccGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACggtggt  <  2:619814/140‑1 (MQ=255)                                                                                                                                     ccGAACAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACggtggt  <  1:321704/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        AATGTGGCGTTTCTTATTGTCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGT  >  NC_000913/3545736‑3546007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑