Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,277,527	G→A	R20H (CGC→CAC)	soxR →	DNA‑binding transcriptional dual regulator SoxR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,277,527	0	G	A	100.0%	271.3 / NA	77	R20H (CGC→CAC)	soxR	DNA‑binding transcriptional dual regulator SoxR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/40);  total (37/40)

CCTCTTTTCAGTGTTCAGTTCGTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACGCAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTC  >  NC_000913/4277391‑4277658                                                                                                                                         |                                                                                                                                    ccTCTTTTCAGTGTTCAGTTCGTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAg                                                                                                                                  >  1:683233/1‑140 (MQ=255)  ctctTTTCAGTGTTCAGTTCGTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGc                                                                                                                                 >  1:614895/1‑140 (MQ=255)    ctTTTCAGTGTTCAGTTCGTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCgg                                                                                                                               >  1:8493/1‑140 (MQ=255)     ttttCAGTGTTCAGTTCGTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGgt                                                                                                                              <  2:319518/140‑1 (MQ=255)                   ttCGTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGgc                                                                                                                >  2:1173467/1‑140 (MQ=255)                   ttCGTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGgc                                                                                                                >  1:882902/1‑140 (MQ=255)                    tCGTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGgcg                                                                                                               >  2:121166/1‑140 (MQ=255)                    tCGTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGgcg                                                                                                               <  2:584263/140‑1 (MQ=255)                    tCGTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGgcg                                                                                                               <  1:121166/140‑1 (MQ=255)                      gTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCt                                                                                                             >  1:912343/1‑140 (MQ=255)                      gTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCt                                                                                                             >  1:903814/1‑140 (MQ=255)                        tAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGc                                                                                                           >  2:243703/1‑140 (MQ=255)                        tAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGc                                                                                                           >  2:1177937/1‑140 (MQ=255)                          aTTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCAt                                                                                                         <  2:69497/140‑1 (MQ=255)                               tCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTa                                                                                                    >  1:622000/1‑140 (MQ=255)                                cTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTAt                                                                                                   <  2:527113/140‑1 (MQ=255)                                  gTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGa                                                                                                 <  1:215929/140‑1 (MQ=255)                                  gTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGa                                                                                                 <  2:265822/140‑1 (MQ=255)                                      gggAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCt                                                                                                             <  1:827874/124‑1 (MQ=255)                                      gggAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCt                                                                                                             >  2:827874/1‑124 (MQ=255)                                        gAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTaa                                                                                           >  1:347398/1‑140 (MQ=255)                                          gTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAg                                                                                         <  2:94568/140‑1 (MQ=255)                                           tataATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAgg                                                                                        >  1:431264/1‑140 (MQ=255)                                            ataATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAggg                                                                                       <  2:8493/140‑1 (MQ=255)                                             taATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAg                                                                                         <  2:1156070/137‑1 (MQ=255)                                             taATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAg                                                                                         >  1:1156070/1‑137 (MQ=255)                                             taATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGt                                                                                      >  1:271962/1‑140 (MQ=255)                                               aTTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTg                                                                                    <  1:243703/140‑1 (MQ=255)                                                     cAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGa                                                                                   >  1:285310/1‑135 (MQ=255)                                                     cAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGa                                                                                   <  2:285310/135‑1 (MQ=255)                                                     cAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTAcc                                                                              >  2:781343/1‑140 (MQ=255)                                                     cAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTAcc                                                                              >  2:1193932/1‑140 (MQ=255)                                                     cAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTAcc                                                                              >  2:619360/1‑140 (MQ=255)                                                     cAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTAcc                                                                              >  1:197671/1‑140 (MQ=255)                                                     cAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTAcc                                                                              >  1:141921/1‑140 (MQ=255)                                                     cAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTAcc                                                                              >  2:505385/1‑140 (MQ=255)                                                     cAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGGTGATTAcc                                                                              >  2:92128/1‑140 (MQ=255)                                                     cAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAAGGGTGATTAcc                                                                              >  2:60153/1‑140 (MQ=255)                                                     cAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGGATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTAcc                                                                              >  2:665584/1‑140 (MQ=255)                                                      aaGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCa                                                                             <  2:118518/140‑1 (MQ=255)                                                      aaGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCa                                                                             <  2:271962/140‑1 (MQ=255)                                                      aaGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCa                                                                             <  2:683233/140‑1 (MQ=255)                                                      aaGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCa                                                                             <  1:505385/140‑1 (MQ=255)                                                        gTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGt                                                                           <  1:619360/140‑1 (MQ=255)                                                         ttAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTa                                                                          <  1:214561/140‑1 (MQ=255)                                                             cTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCg                                                                      >  1:1032787/1‑140 (MQ=255)                                                              ttGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGt                                                                     <  1:309435/140‑1 (MQ=255)                                                                  ggTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCa                                                                             >  1:160620/1‑128 (MQ=255)                                                                  ggTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCa                                                                             <  2:160620/128‑1 (MQ=255)                                                                  ggTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACa                                                                 <  2:1211970/140‑1 (MQ=255)                                                                       aGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGc                                                            >  1:497217/1‑140 (MQ=255)                                                                           aTTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATc                                                        >  1:1207113/1‑140 (MQ=255)                                                                             ttATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAg                                                                <  1:1102829/130‑1 (MQ=255)                                                                             ttATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAg                                                                >  2:1102829/1‑130 (MQ=255)                                                                             ttATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGGTGATTACCAGTATCCGTAACAGCGGCAATCAg                                                      >  1:614738/1‑140 (MQ=255)                                                                                tGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAgcgg                                                   <  2:497217/140‑1 (MQ=255)                                                                                  gAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAgcggcg                                                 <  1:1218929/140‑1 (MQ=255)                                                                                        aaaTTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATaa                                           <  2:1032787/140‑1 (MQ=255)                                                                                             aCCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTg                                      <  1:654506/140‑1 (MQ=255)                                                                                             aCCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTg                                      <  1:881838/140‑1 (MQ=255)                                                                                             aCCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTg                                      <  1:817483/140‑1 (MQ=255)                                                                                             aCCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGAAAAAGGGTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTg                                      >  2:1218929/1‑140 (MQ=255)                                                                                                  gCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGAtgtg                                 <  2:141921/140‑1 (MQ=255)                                                                                                   cATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGGATCCGGAAAAGCGGCAATCAGGGGCGATATAAACGTGATttgt                                >  2:817483/1‑140 (MQ=255)                                                                                                     ttAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTg                              <  1:718402/140‑1 (MQ=255)                                                                                                     ttAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTg                              <  2:347398/140‑1 (MQ=255)                                                                                                     ttAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTg                              <  2:912343/140‑1 (MQ=255)                                                                                                       aaaGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTGCg                            <  1:1193932/140‑1 (MQ=255)                                                                                                         aGCGCTGCTAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTGCGat                          <  2:903814/140‑1 (MQ=255)                                                                                                            gctgctAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATg                       <  2:1106998/140‑1 (MQ=255)                                                                                                             ctgctAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGt                      >  1:636408/1‑140 (MQ=255)                                                                                                                ctAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCCTTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGc                   >  2:864236/1‑140 (MQ=255)                                                                                                                 tAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCa                  <  2:622000/140‑1 (MQ=255)                                                                                                                 tAACCCCCGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCa                  <  1:1177937/140‑1 (MQ=255)                                                                                                                        cGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCa           >  1:68671/1‑140 (MQ=255)                                                                                                                        cGGCGAAGTGGCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCa           <  2:995016/140‑1 (MQ=255)                                                                                                                                 ggCGAAACACAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTc  <  2:917059/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CCTCTTTTCAGTGTTCAGTTCGTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTATGGAAAAGAAATTACCCCGCATTAAAGCGCTGCTAACCCCCGGCGAAGTGGCGAAACGCAGCGGTGTGGCGGTATCGGCGCTGCATTTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTC  >  NC_000913/4277391‑4277658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑