| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,206,435 | 0 | C | T | 33.3% | 36.0 / 14.4 | 27 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/18); new base T (9/0); total (9/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.13e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAA > NC_000913/3206305‑3206574 | tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTTACAC‑AAAt > 2:1159987/1‑140 (MQ=255) ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGa < 2:720197/140‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAg < 1:1181837/140‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAg < 1:616689/140‑1 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGAC‑AAAAACAAAAAAAGGAGGTGc > 2:1181837/1‑140 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAC‑AAAAACAAAAAAATGAGTTGc > 1:1371795/1‑140 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTAACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAC‑AAATACAAAAACTGGAGTTGc > 1:1181903/1‑140 (MQ=255) tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAg < 2:483940/140‑1 (MQ=255) attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAC‑AAAAAAAAAAAAAGGAGGTGGCATGATGAggg > 2:958836/1‑138 (MQ=255) ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGa < 1:958836/140‑1 (MQ=255) ttATTTTCGCGTTGATTAACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAg < 2:1181903/140‑1 (MQ=255) tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAC‑AAAAAAAAAAAATTGAGGTGCCATGATGAGCGAAAga > 1:178014/1‑139 (MQ=255) tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAC‑AAAAAAAAAAAATGGAGTTGCCATGATGAGCGAAAga > 1:507899/1‑139 (MQ=255) tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat < 2:993203/140‑1 (MQ=255) tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat < 1:700217/140‑1 (MQ=255) gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCTGAC‑AAAAAAAAAAAATGGAGGTTGCATGATGAGGGAGAGaaataaaa > 2:864024/1‑138 (MQ=255) gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAGAC‑AAATACAAAAACTGGGGTTGGCCTGATGAGCGGAAAgaataaaa > 2:1269450/1‑138 (MQ=255) agacagcACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc < 1:364678/134‑1 (MQ=255) aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCTCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg < 2:372803/140‑1 (MQ=255) cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAg < 1:1159987/140‑1 (MQ=255) gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg < 1:864024/140‑1 (MQ=255) gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg < 2:936170/140‑1 (MQ=255) atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAC‑AAAAAAAAAAAATGGAGTTGGCATGATGAGCGAAAAAAAAAAAAAACAAGCAGAGAAAAAAATGAAagag > 2:1321757/1‑140 (MQ=255) gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt < 1:1321757/140‑1 (MQ=255) gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAC‑AAAAAAAAAAAATGGAGGTGGCATGATGAGCGAAAGTAATAAACAACAAGCAGTGAATAAAATGACAGAGAATGTTGCTaaa > 2:723725/1‑139 (MQ=255) taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAGACAAAAAAAAAAAAATGAGGTTGCC‑TGATGAGGGAAAATAAAAAAAAACAAGCAGTGAATAAATTTACAGAGAATGTCGCTAACTTTAACGccc > 2:296313/1‑139 (MQ=255) caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAC‑AAAAAAAAAAACAGGAGGTGGCATGATGAGGGAAAGAAAAAAAAAAAAAGCAGAGAATAAAATGACAGAGATTGTCGGTAAATTTACCGCCATGAttttt > 2:756696/1‑138 (MQ=255) aaTTTTTAGTGGTAGTTTGTGGCGGTAAATTTTCCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATAAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTATCTTTACCGCCATGATTTCt < 2:257909/139‑1 (MQ=255) aGTGGTAGTTTGTGGGGGTGATTTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa < 1:296313/140‑1 (MQ=255) aGTGGTAGTTTGTGGCGGTGAATTTTTTCAGGC‑AAAAAAAAAAAATGGAGGTGCCATGATGAGGGGAAGGAATAAAAAAAAAGGAGAGAAAAAAATTACAGAGATTGTCGCTTACATTTCCGCCATTGTTTCTTCCCCaa > 2:831207/1‑140 (MQ=255) gTGGTAGTTTGTGGCGGTGAATTTTTCCAAAC‑AAAAAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt < 2:1371795/140‑1 (MQ=255) agacaggTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt < 1:465439/134‑1 (MQ=255) ttGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAATTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCtgatg < 2:178014/140‑1 (MQ=255) tgtgGCGGTGAATTTTTTCCGAC‑AAAAAAAAAAAATGGAGGTGCCATGATGAGCGGAAGTAATAAACAAAAAGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTACCCCCATGATTTTTACCCCAATGCCtgatta > 2:1252642/1‑138 (MQ=255) tGAATTTTTTCAGAC‑AAAAACAAAAAATGGAGGTGCCATGATGAGCGAAAGAAAAAAACAAAAAGCAGAGAATAAAATGACAGAGATTTTTGCTAACTTTACCCCCATGATTTCTACCCCAATGCCTGATGACgctgtct > 2:465439/1‑134 (MQ=255) gAATTTTTCAAGAC‑AAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa < 2:507899/140‑1 (MQ=255) aaaTTTTTCCAGAA‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa < 1:250534/139‑1 (MQ=255) ccAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa < 1:1186272/140‑1 (MQ=255) ccAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa > 2:1186272/1‑140 (MQ=255) | TGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAA > NC_000913/3206305‑3206574 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |