| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,825,061 | 0 | G | A | 26.1% | 91.2 / 16.4 | 46 | intergenic (‑124/+79) | ves/spy | HutD family protein Ves/ATP‑independent periplasmic chaperone |
| Reads supporting (aligned to +/- strand): ref base G (31/3); new base A (0/12); total (31/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.17e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGA > NC_000913/1824933‑1825191 | tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGa > 2:577966/1‑140 (MQ=255)tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGa > 1:271750/1‑140 (MQ=255)tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGa > 1:181554/1‑140 (MQ=255) tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTc > 2:954785/1‑140 (MQ=255) ccccccccGGCCTTTTTTTTTCTTTTTTATCGTTCAGGTACCGGTGGATGGTTATCGGCAACGGGGGTGTTTTACGTTGGTGGTGATCAGTTTTTTTTTACAACTTTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTc < 2:134451/135‑1 (MQ=255) gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCa > 1:268369/1‑140 (MQ=255) cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATccctgtctc > 1:629838/1‑133 (MQ=255) tCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAcc > 1:142253/1‑140 (MQ=255) cctttttttttCTTTTTTATCGTTCAGGTACCGGGGGATGGTTACCGGCACGGGGGGTGATTTGCTTTAGGGGTGACCAGTTTTTTTTAACACTTTTTGGAAAAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTa < 2:2128211/133‑1 (MQ=255) cccTTTTTTTTCTTTGTTATCGTTCGGGTAACCGGGGATGGTTATGGGCAACTGGGGTGATTTACGTTAGGGGTGATCAGGTTTTTTTAACAATTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTa < 2:265765/140‑1 (MQ=255) cccTTTTTTTTCATTGTTATCGTTCAGTTACCGGTGGTTGTTTATCGGCAACTGGGGTGATTTACGTTGGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTa < 2:268369/140‑1 (MQ=255) ttttttCTTTTTTTTCGTTCAGGTAGCTGTGGTTGGTTATCGGCACTTGGGGTGATTTACTTTAGTGGTGATCAGGTTTTTTTTACAATTTTTGGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCa < 2:1692952/138‑1 (MQ=255) ttttttCTTTTTTATCTTTCAGGTAACCGTGGATGGTTATCGGCAACTGGGGTGATTTACGTTGGGGGTGATCAGGTTTTTTTTACAATTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCa < 2:271750/138‑1 (MQ=255) ttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgct > 1:129294/1‑138 (MQ=255) ttATCGTTCAGGTAACCGTGGATGTTTTGCGGCATTTGGGGTGATTTAATTTGGGGGTGATCGGGTTTTTTTTACATTTTTTGGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc < 2:2045957/140‑1 (MQ=255) ttATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc > 2:362706/1‑140 (MQ=255) tCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg > 1:1001355/1‑140 (MQ=255) ttCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc > 1:2045957/1‑140 (MQ=255) cAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg > 2:719979/1‑140 (MQ=255) ggTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg > 2:711451/1‑140 (MQ=255) ggTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGCAGTGGACAAGACcggcg > 1:1410091/1‑140 (MQ=255) aCCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCt > 1:591894/1‑140 (MQ=255) ccGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt > 1:284567/1‑140 (MQ=255) ggTGGATGTTTACCGGAAACTGGGGTGTTTTACGTTAGGGGTGATCGGTTTTTTTTTAAAACTGTTTAAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa < 2:1001355/139‑1 (MQ=255) ggATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGt > 1:1662549/1‑140 (MQ=255) gATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt > 1:1197845/1‑140 (MQ=255) gATGGTTATCGGCAACTCGGGTGATTTACGTTAGCGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt > 1:1316304/1‑140 (MQ=255) aTCGGAAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACATCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc < 1:577966/140‑1 (MQ=255) aTCGACATCTGGGGTATTTTACTTTAGTGGTGTTGGGTTTTTTTTTACAACTGTTGGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc < 2:1197845/140‑1 (MQ=255) tCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCt > 1:1109278/1‑140 (MQ=255) tCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCt > 1:1655278/1‑140 (MQ=255) gggCAACTGGGGTGTTTTATTTTAGTGGTGATCAGTTTTTTTTAACACTTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg < 2:1655278/139‑1 (MQ=255) gggCAACGGGGGTGATTTACGTTAGGGGTGCTCGGGTTTTTTTTAAAATTGTTAGAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg < 2:1201736/139‑1 (MQ=255) cttggggTGTTTTACGTTGGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGa < 1:1238796/136‑1 (MQ=255) aaTTTAGGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCCTTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTcag < 2:1247431/139‑1 (MQ=255) tttgctttAGTGGGGTTCGGGTTTTTTTTACAATTGTTTGAGAAGAAAAAAAAAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTcagca < 1:362706/134‑1 (MQ=255) ttgggggtgATCGGTTTTTTTTTAAAATTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTCAGCAGTTGCa < 2:2030106/135‑1 (MQ=255) agacaggtgATCGGGTTTTTTTTACAACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTCAGCAGTTGCa < 2:1754579/135‑1 (MQ=255) tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTA‑TTCAGCAGTTGCAGGCa > 2:1514716/1‑140 (MQ=255) tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTCAGCAGTTGCAGGcc > 1:1247431/1‑139 (MQ=255) gtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTTCTGAAAAATTA‑TTCAGCAGTTGCAGGCAtt > 2:2106366/1‑140 (MQ=255) cAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGCTGAAAAATTATTTCAG‑AGTTGCAGGGCTTTTaac > 2:1022359/1‑138 (MQ=255) ggCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTCAGCAGTTGCAGGCATTTTACCtt > 1:1561282/1‑140 (MQ=255) gCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTCAGCAGTTGCAGGCATTTTACCttt > 1:336548/1‑140 (MQ=255) ttttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTCAGCAGTTGCAGGCATTTTAccctgtct > 1:1754579/1‑134 (MQ=255) ttttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTCAGCAGTTGCAGGCATTTTACCTTTTGc > 1:1156555/1‑140 (MQ=255) tttttACAACTTTTTAAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTCAGCAGTTGCAGGCATTTTACCtgcaact < 2:776582/140‑7 (MQ=255) tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTA‑TTTAGCAGTTGCAGGCATTTTTCCTTTTTCCGCTGGGCGTTCt > 1:1068904/1‑140 (MQ=255) tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt > 1:43439/1‑140 (MQ=255) agaggacaaAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCa < 2:1410091/140‑1 (MQ=255) aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGa < 2:591894/140‑1 (MQ=255) | TCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTA‑TTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGA > NC_000913/1824933‑1825191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |