Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,620,240	A→G	*300Q (TAA→CAA)	eamA ←	cysteine/O‑acetylserine exporter EamA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,620,240	0	A	G	100.0%	263.1 / NA	67	*300Q (TAA→CAA)	eamA	cysteine/O‑acetylserine exporter EamA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/29);  total (38/29)

CATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCC  >  NC_000913/1620107‑1620375                                                                                                                                      |                                                                                                                                        cATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTcc                                                                                                                                   <  2:1159499/140‑1 (MQ=255)    cGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACtt                                                                                                                                     <  1:762673/135‑1 (MQ=255)    cGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACtt                                                                                                                                     >  2:762673/1‑135 (MQ=255)         gAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTa                                                                                                                           <  1:986993/140‑1 (MQ=255)          aaCACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTAc                                                                                                                          <  1:765578/140‑1 (MQ=255)            cacccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCg                                                                                                                        >  2:528234/1‑140 (MQ=255)              cccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTAc                                                                                                                          <  1:217188/136‑1 (MQ=255)              cccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTAc                                                                                                                          >  2:217188/1‑136 (MQ=255)                 accGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTa                                                                                                                   >  2:1206370/1‑140 (MQ=255)                    gTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGc                                                                                                                <  2:789412/140‑1 (MQ=255)                       tGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCAc                                                                                                             >  2:91518/1‑140 (MQ=255)                               aTCACATGGGTACTGGCAGTGATAAGTAGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGcc                                                                                                     >  1:133872/1‑140 (MQ=255)                                    aTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                                                                >  2:411829/1‑140 (MQ=255)                                    aTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                                                                <  1:528234/140‑1 (MQ=255)                                    aTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                                                                <  1:411829/140‑1 (MQ=255)                                    aTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                                                                <  2:1072997/140‑1 (MQ=255)                                    aTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                                                                >  2:577824/1‑140 (MQ=255)                                      gggTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACa                                                                                              <  1:577824/140‑1 (MQ=255)                                         tACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                                                                <  1:226908/135‑1 (MQ=255)                                         tACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                                                                >  2:226908/1‑135 (MQ=255)                                           cTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGat                                                                                         >  2:685758/1‑140 (MQ=255)                                              gCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                                                                <  2:694750/130‑1 (MQ=255)                                              gCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                                                                >  1:694750/1‑130 (MQ=255)                                                  tGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGc                                                                                  >  1:170142/1‑140 (MQ=255)                                                   gATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGcc                                                                                 <  1:1073976/140‑1 (MQ=255)                                                   gATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGcc                                                                                 <  2:1200613/140‑1 (MQ=255)                                                      aaGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCgg                                                                              <  1:151832/140‑1 (MQ=255)                                                        gTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTc                                                                            <  1:685758/140‑1 (MQ=255)                                                        gTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTc                                                                            >  1:502925/1‑140 (MQ=255)                                                            gATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATaa                                                                        >  2:718321/1‑140 (MQ=255)                                                                  cTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGcc                                                                                 >  2:87580/1‑125 (MQ=255)                                                                  cTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGcc                                                                                 <  1:87580/125‑1 (MQ=255)                                                                                     aTCAACACGCTATGTAGTTTGTTCTGGCCCCGGCATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAg                                               >  2:496940/1‑140 (MQ=255)                                                                                      tCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTg                                                  >  2:946920/1‑136 (MQ=255)                                                                                      tCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTg                                                  <  1:946920/136‑1 (MQ=255)                                                                                      tCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGa                                              <  1:843890/140‑1 (MQ=255)                                                                                      tCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGa                                              >  1:841071/1‑140 (MQ=255)                                                                                        aaCACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAcc                                            >  1:894720/1‑140 (MQ=255)                                                                                          cacGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCg                                          >  2:498431/1‑140 (MQ=255)                                                                                          cacGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCg                                          >  1:1095268/1‑140 (MQ=255)                                                                                          cacGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCg                                          >  1:1185880/1‑140 (MQ=255)                                                                                          cacGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCg                                          >  2:769390/1‑140 (MQ=255)                                                                                          cacGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCg                                          >  1:362910/1‑140 (MQ=255)                                                                                          cacGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCg                                          >  2:403817/1‑140 (MQ=255)                                                                                          cacGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCg                                          >  1:475835/1‑140 (MQ=255)                                                                                            cGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGtt                                        <  1:718321/140‑1 (MQ=255)                                                                                               tATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAg                                     >  2:767925/1‑140 (MQ=255)                                                                                                 tGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCg                                   >  1:570814/1‑140 (MQ=255)                                                                                                   tAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGtt                                 <  2:170142/140‑1 (MQ=255)                                                                                                     gTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCa                               <  1:403817/140‑1 (MQ=255)                                                                                                         gTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATc                             <  2:1251861/138‑1 (MQ=255)                                                                                                         gTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATc                             >  1:1251861/1‑138 (MQ=255)                                                                                                         gTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCca                           >  1:1061577/1‑140 (MQ=255)                                                                                                          ttCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCcaa                          <  2:133872/140‑1 (MQ=255)                                                                                                          ttCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCcaa                          >  1:1118511/1‑140 (MQ=255)                                                                                                           tCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCcaac                         >  1:741146/1‑140 (MQ=255)                                                                                                           tCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCcaac                         >  2:209158/1‑140 (MQ=255)                                                                                                               gCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATa                     >  1:2524/1‑140 (MQ=255)                                                                                                               gCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATa                     >  2:796977/1‑140 (MQ=255)                                                                                                                ccccGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAg                    >  2:557665/1‑140 (MQ=255)                                                                                                                    gACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCa                               >  1:1251877/1‑125 (MQ=255)                                                                                                                    gACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCa                               <  2:1251877/125‑1 (MQ=255)                                                                                                                    gACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGcc                >  1:1323566/1‑140 (MQ=255)                                                                                                                     aCATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCg               <  2:841071/140‑1 (MQ=255)                                                                                                                       aTCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCa             <  1:209158/140‑1 (MQ=255)                                                                                                                            ggggCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGc        <  1:1206370/140‑1 (MQ=255)                                                                                                                            ggggCTTATTGACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGc        <  1:91518/140‑1 (MQ=255)                                                                                                                                  tattgACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTcc  <  2:502925/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        CATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCC  >  NC_000913/1620107‑1620375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑