Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,710,640	C→A	A146E (GCG→GAG)	rsxG →	SoxR [2Fe‑2S] reducing system protein RsxG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,710,640	0	C	A	100.0%	201.0 / NA	58	A146E (GCG→GAG)	rsxG	SoxR [2Fe‑2S] reducing system protein RsxG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/28);  total (30/28)

CGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTAAAACGCGCCGGATTGTACGC  >  NC_000913/1710506‑1710775                                                                                                                                       |                                                                                                                                        cGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTa                                                                                                                                    >  2:372778/1‑140 (MQ=255)  ggTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTaa                                                                                                                                   >  1:581985/1‑140 (MQ=255)    tGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTaaaa                                                                                                                                 >  2:1120107/1‑140 (MQ=255)           cAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAg                                                                                                                          <  1:1120107/140‑1 (MQ=255)           cAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAg                                                                                                                          <  1:693537/140‑1 (MQ=255)           cAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAg                                                                                                                          <  1:258507/140‑1 (MQ=255)            aGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAgt                                                                                                                         <  1:1312522/140‑1 (MQ=255)                   ggtggGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAg                                                                                                                  <  2:284237/140‑1 (MQ=255)                   ggtggGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAg                                                                                                                  <  2:824929/140‑1 (MQ=255)                   ggtggGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAg                                                                                                                  <  1:445102/140‑1 (MQ=255)                        gAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAgatgat                                                                                                             <  2:626454/140‑1 (MQ=255)                           ccGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAgatgat                                                                                                             >  2:243800/1‑137 (MQ=255)                           ccGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAgatgat                                                                                                             <  1:243800/137‑1 (MQ=255)                           ccGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATgcg                                                                                                          >  2:1145828/1‑140 (MQ=255)                           ccGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATgc                                                                                                           <  1:1302169/139‑1 (MQ=255)                           ccGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATgc                                                                                                           >  2:1302169/1‑139 (MQ=255)                                 ttAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGTGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTgg                                                                                                    >  2:1299310/1‑140 (MQ=255)                                    aCGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCg                                                                                                 <  1:1145828/140‑1 (MQ=255)                                        cACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTga                                                                                             >  1:998363/1‑140 (MQ=255)                                         aCGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTgaa                                                                                            >  1:278111/1‑140 (MQ=255)                                                ttGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCC‑TTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGAtg                                                                                    >  2:1085252/1‑140 (MQ=255)                                                  ggCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGc                                                                                                  >  1:875730/1‑125 (MQ=255)                                                  ggCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGc                                                                                                  <  2:875730/125‑1 (MQ=255)                                                  ggCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGAtgg                                                                                   >  1:629611/1‑140 (MQ=255)                                                    cACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGAtggtg                                                                                 <  1:372778/140‑1 (MQ=255)                                                       gcgcgTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGgtggtg                                                                              >  2:1150785/1‑140 (MQ=255)                                                       gcgcgTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGgtggtg                                                                              <  1:283200/140‑1 (MQ=255)                                                       gcgcgTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGgtggtg                                                                              >  2:1100328/1‑140 (MQ=255)                                                         gcgTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGAt                                                                            >  1:481275/1‑140 (MQ=255)                                                               cAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGAc                                                                      <  2:998363/140‑1 (MQ=255)                                                               cAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCACACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGAc                                                                      <  1:1165543/140‑1 (MQ=255)                                                                         cGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCg                                                            >  2:418798/1‑140 (MQ=255)                                                                         cGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCg                                                            >  1:1046819/1‑140 (MQ=255)                                                                         cGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCg                                                            >  1:672066/1‑140 (MQ=255)                                                                             aCGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGgcgc                                                        <  2:672066/140‑1 (MQ=255)                                                                             aCGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGgcgc                                                        <  2:629611/140‑1 (MQ=255)                                                                              cGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGcgcg                                                       <  2:1046819/140‑1 (MQ=255)                                                                               gCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGcga                                                      <  1:860565/140‑1 (MQ=255)                                                                               gCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGcga                                                      <  1:28897/140‑1 (MQ=255)                                                                                   gggCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGAt                                                  <  2:581985/140‑1 (MQ=255)                                                                                    ggCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGAtt                                                 >  2:1016306/1‑140 (MQ=255)                                                                                        tGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTc                                             >  1:940686/1‑140 (MQ=255)                                                                                         ggCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTcc                                            >  1:718202/1‑140 (MQ=255)                                                                                           cGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTcccc                                          >  1:927013/1‑140 (MQ=255)                                                                                              tAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATATCGACCAGTTCACCGGCGCGACGATTACTCCCcgcg                                       <  2:481275/140‑1 (MQ=255)                                                                                               aaaaTCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTcccc                                          >  2:897294/1‑136 (MQ=255)                                                                                               aaaaTCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTcccc                                          <  1:897294/136‑1 (MQ=255)                                                                                                aaaTCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCcgcgcg                                     <  2:312021/140‑1 (MQ=255)                                                                                                     gAACTGCGCCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCggtggt                                <  2:278111/140‑1 (MQ=255)                                                                                                          gcgcCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATg                           <  2:957046/140‑1 (MQ=255)                                                                                                           cgcCTTTCTGACTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGc                          >  2:619814/1‑140 (MQ=255)                                                                                                                     aCTGGATCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTAAAAcgc                >  2:968130/1‑140 (MQ=255)                                                                                                                          aTCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTaa                             <  2:780597/122‑1 (MQ=255)                                                                                                                          aTCACCCATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTaa                             >  1:780597/1‑122 (MQ=255)                                                                                                                               ccATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTAAAACGCGCCGGATTGt      >  1:1297662/1‑140 (MQ=255)                                                                                                                               ccATTTTGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTAAAACGCGCCGGATTGt      >  1:990386/1‑140 (MQ=255)                                                                                                                                   tttGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTAAAACGCGCCGGATTGTACGc  >  1:1326381/1‑140 (MQ=255)                                                                                                                                   tttGAGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTAAAACGCGCCGGATTGTACGc  >  2:118228/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        CGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTAAAACGCGCCGGATTGTACGC  >  NC_000913/1710506‑1710775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑