Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,373,638	T→C	intergenic (‑62/+60)	nanA ← / ← nanR	N‑acetylneuraminate lyase/DNA‑binding transcriptional dual regulator NanR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,373,638	0	T	C	100.0%	321.0 / NA	81	intergenic (‑62/+60)	nanA/nanR	N‑acetylneuraminate lyase/DNA‑binding transcriptional dual regulator NanR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/36);  total (45/36)

CTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGTTGAGATGCGATTGCAACGC  >  NC_000913/3373504‑3373772                                                                                                                                       |                                                                                                                                       cTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAAtt                                                                                                                                   >  2:1057961/1‑140 (MQ=255)      tCCGGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCa                                                                                                                              <  1:1187793/140‑1 (MQ=255)      tCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCa                                                                                                                              <  1:962176/140‑1 (MQ=255)      tCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCa                                                                                                                              <  1:1133073/140‑1 (MQ=255)         aGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAaca                                                                                                                           <  1:968337/140‑1 (MQ=255)           tGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACAcc                                                                                                                         >  1:907736/1‑140 (MQ=255)           tGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACAcc                                                                                                                         <  1:745088/140‑1 (MQ=255)            gCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTAAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCa                                                                                                                              >  1:817773/1‑134 (MQ=255)            gCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTAAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCa                                                                                                                              <  2:817773/134‑1 (MQ=255)                gttgttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCaa                                                                                                                             <  1:1292066/131‑1 (MQ=255)                gttgttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCaa                                                                                                                             >  2:1292066/1‑131 (MQ=255)                gttgttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATa                                                                                                                    >  1:339208/1‑140 (MQ=255)                gttgttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATa                                                                                                                    >  2:767783/1‑140 (MQ=255)                   gttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAg                                                                                                                 <  1:1029339/140‑1 (MQ=255)                   gttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAg                                                                                                                 <  1:744041/140‑1 (MQ=255)                    ttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGa                                                                                                                <  2:655288/140‑1 (MQ=255)                     tgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGaa                                                                                                               >  2:207771/1‑140 (MQ=255)                       gTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATAc                                                                                                                   >  1:844504/1‑134 (MQ=255)                       gTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATAc                                                                                                                   <  2:844504/134‑1 (MQ=255)                        tCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGc                                                                                                            >  2:1045305/1‑140 (MQ=255)                          aaaaGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCtt                                                                                                          <  1:797572/140‑1 (MQ=255)                                 gTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGc                                                                                                   >  2:20938/1‑140 (MQ=255)                                    aGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGAt                                                                                                <  2:1256267/140‑1 (MQ=255)                                      gAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCt                                                                                              >  2:100039/1‑140 (MQ=255)                                       aGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTg                                                                                             <  1:664555/140‑1 (MQ=255)                                       aGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATAACTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTg                                                                                             <  2:861618/140‑1 (MQ=255)                                          gCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCtt                                                                                          <  1:207771/140‑1 (MQ=255)                                           cAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTc                                                                                         >  1:217103/1‑140 (MQ=255)                                                   aCGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGt                                                                                 <  2:594042/140‑1 (MQ=255)                                                   aCGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGt                                                                                 >  1:594042/1‑140 (MQ=255)                                                         cGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCatt                                                                           >  1:863719/1‑140 (MQ=255)                                                         cGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCatt                                                                           >  2:34877/1‑140 (MQ=255)                                                         cGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCa                                                                             <  1:1215156/138‑1 (MQ=255)                                                         cGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCa                                                                             >  2:1215156/1‑138 (MQ=255)                                                          gTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCatta                                                                          >  1:1132991/1‑140 (MQ=255)                                                                   ttGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCttttt                                                                 >  1:219986/1‑140 (MQ=255)                                                                    tGCCATAAATACCTCTGAAGTGATGCTTGTATGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTttg                                                                >  2:414616/1‑140 (MQ=255)                                                                     gCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTttgt                                                               >  2:30824/1‑140 (MQ=255)                                                                            aTACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTggtggt                                                        <  2:339208/140‑1 (MQ=255)                                                                                ctctGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGa                                                    >  2:572782/1‑140 (MQ=255)                                                                                ctctGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTACACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGa                                                    >  1:383527/1‑140 (MQ=255)                                                                                     aaGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGaa                                               >  2:412442/1‑140 (MQ=255)                                                                                     aaGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGaa                                               >  2:413276/1‑140 (MQ=255)                                                                                     aaGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGa                                                >  1:687832/1‑139 (MQ=255)                                                                                     aaGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGa                                                <  2:687832/139‑1 (MQ=255)                                                                                        tGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGc                                            <  1:767783/140‑1 (MQ=255)                                                                                          aTGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCg                                           <  1:744391/139‑1 (MQ=255)                                                                                          aTGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCg                                           >  2:744391/1‑139 (MQ=255)                                                                                          aTGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGt                                          >  1:773518/1‑140 (MQ=255)                                                                                          aTGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGt                                          >  2:1103439/1‑140 (MQ=255)                                                                                          aTGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGt                                          >  2:390165/1‑140 (MQ=255)                                                                                          aTGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGt                                          >  1:2156/1‑140 (MQ=255)                                                                                           tGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTg                                         <  2:1132991/140‑1 (MQ=255)                                                                                               tGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAg                                     >  1:914296/1‑140 (MQ=255)                                                                                               tGTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAg                                     >  2:323721/1‑140 (MQ=255)                                                                                                gTCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAgg                                    >  2:941385/1‑140 (MQ=255)                                                                                                 tCTGATAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGt                                   >  1:310693/1‑140 (MQ=255)                                                                                                    gATAAACGATATACCTTTATACCTGTTACACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGc                                <  2:383527/140‑1 (MQ=255)                                                                                                     aTAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCa                               >  1:49341/1‑140 (MQ=255)                                                                                                      tAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTg                                         >  1:612511/1‑129 (MQ=255)                                                                                                      tAAACGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTg                                         <  2:612511/129‑1 (MQ=255)                                                                                                         aCGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAgaga                           <  2:914296/140‑1 (MQ=255)                                                                                                         aCGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAgaga                           <  1:572782/140‑1 (MQ=255)                                                                                                         aCGATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAgaga                           <  1:413276/140‑1 (MQ=255)                                                                                                           gATATACCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACg                         <  1:323721/140‑1 (MQ=255)                                                                                                             tataCCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCt                       >  2:1088363/1‑140 (MQ=255)                                                                                                               taCCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAgaga                           <  1:110154/134‑1 (MQ=255)                                                                                                               taCCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAgaga                           >  2:110154/1‑134 (MQ=255)                                                                                                               taCCTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGt                     >  1:180302/1‑140 (MQ=255)                                                                                                                  cTTTATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGTTga                  <  2:773518/140‑1 (MQ=255)                                                                                                                    ttATACCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGTTgaga                >  2:814183/1‑140 (MQ=255)                                                                                                                     tataCCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGTTGAGAt               >  1:685061/1‑140 (MQ=255)                                                                                                                       taCCTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGTTGAGATGc             >  1:447688/1‑140 (MQ=255)                                                                                                                         ccTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGTTGAGATGCGa           <  1:414616/140‑1 (MQ=255)                                                                                                                         ccTGTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGTTGAGATGCGa           >  1:304392/1‑140 (MQ=255)                                                                                                                            gTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGTTGAGATGCGATTg        >  2:482018/1‑140 (MQ=255)                                                                                                                            gTTATACCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGTTGAGATGCGATTg        >  1:1118721/1‑140 (MQ=255)                                                                                                                                taCCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGTTGAGATGCGATTGCAAc    <  1:482018/140‑1 (MQ=255)                                                                                                                                 aCCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGTTGAGATGCGATTGCAACg   <  2:685061/140‑1 (MQ=255)                                                                                                                                 aCCAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGTTGAGATGCGATTGCAACg   <  2:447688/140‑1 (MQ=255)                                                                                                                                  ccAGACCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGTTGAGATGCGATTGCAACGc  <  2:310693/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       CTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGTGGCATTATTTCTTTTTGTTGGTGGTCTGACCGAAAGCGTGCCAGGTAGCAGAGACGCTGTTGAGATGCGATTGCAACGC  >  NC_000913/3373504‑3373772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑