Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	997,479	A→C	F12V (TTT→GTT)	ssuE ←	NADPH‑dependent FMN reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	997,479	0	A	C	100.0%	263.5 / NA	68	F12V (TTT→GTT)	ssuE	NADPH‑dependent FMN reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/33);  total (35/33)

CCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGTCTGTCGGAGAGACAAGAAAATTCCAAATATAAATT  >  NC_000913/997347‑997606                                                                                                                                     |                                                                                                                                ccGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGAc                                                                                                                         >  2:1283137/1‑140 (MQ=255)  cGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACt                                                                                                                        <  2:861/140‑1 (MQ=255)  cGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACt                                                                                                                        <  2:162264/140‑1 (MQ=255)  cGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACt                                                                                                                        <  2:498338/140‑1 (MQ=255)  cGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACt                                                                                                                        >  1:518925/1‑140 (MQ=255)    gACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTAc                                                                                                                      <  2:82856/140‑1 (MQ=255)    gACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTAc                                                                                                                      >  2:672717/1‑140 (MQ=255)         tCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGcc                                                                                                                 >  2:1303716/1‑140 (MQ=255)          cGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCa                                                                                                                <  2:361021/140‑1 (MQ=255)              aCGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGt                                                                                                            >  2:477212/1‑140 (MQ=255)                 aGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTCCATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGtgat                                                                                                         <  1:1037085/140‑1 (MQ=255)                 aGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGtgat                                                                                                         >  1:525936/1‑140 (MQ=255)                 aGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGtgat                                                                                                         >  2:108478/1‑140 (MQ=255)                 aGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGtgat                                                                                                         >  2:981231/1‑140 (MQ=255)                   cATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGc                                                                                                                  >  1:17605/1‑129 (MQ=255)                   cATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGc                                                                                                                  <  2:17605/129‑1 (MQ=255)                   cATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGtgatga                                                                                                       <  1:672717/140‑1 (MQ=255)                   cATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGtgatga                                                                                                       <  1:477212/140‑1 (MQ=255)                   cATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGtgatga                                                                                                       <  1:1303716/140‑1 (MQ=255)                   cATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGtgatga                                                                                                       >  2:738256/1‑140 (MQ=255)                   cATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGtgatga                                                                                                       <  2:90684/140‑1 (MQ=255)                     tAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGaca                                                                                                     >  1:48310/1‑140 (MQ=255)                     tAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGaca                                                                                                     <  1:1283137/140‑1 (MQ=255)                       aaGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACg                                                                                                   <  2:233206/140‑1 (MQ=255)                       aaGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACg                                                                                                   <  1:739854/140‑1 (MQ=255)                         gTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCa                                                                                                 <  2:1057726/140‑1 (MQ=255)                           aGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATa                                                                                               <  2:1078892/140‑1 (MQ=255)                            gATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATAc                                                                                              >  1:931808/1‑140 (MQ=255)                             aTCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATAct                                                                                             >  1:1155472/1‑140 (MQ=255)                               cTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATActct                                                                                           <  1:738256/140‑1 (MQ=255)                               cTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATActct                                                                                           <  1:1152899/140‑1 (MQ=255)                               cTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATActct                                                                                           <  1:981231/140‑1 (MQ=255)                                ttCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATActctc                                                                                          <  1:407068/140‑1 (MQ=255)                                 tCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATActctct                                                                                         >  1:948487/1‑140 (MQ=255)                                  ccGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACtctctc                                                                                        >  1:1099011/1‑140 (MQ=255)                                  ccGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACtctctc                                                                                        >  1:616423/1‑140 (MQ=255)                                    ggggCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCt                                                                                      >  2:451224/1‑140 (MQ=255)                                        cGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTtata                                                                                  >  2:126799/1‑140 (MQ=255)                                                     aTTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCtt                                                                     >  1:1179683/1‑140 (MQ=255)                                                          aGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTACTTATAACCAATTGTTCttttttt                                                                >  2:592330/1‑140 (MQ=255)                                                             gATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGtt                                                             <  2:518925/140‑1 (MQ=255)                                                              aTAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTa                                                            <  2:948487/140‑1 (MQ=255)                                                                 aaCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTg                                                                          <  2:126317/123‑1 (MQ=255)                                                                 aaCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTg                                                                          >  1:126317/1‑123 (MQ=255)                                                                           tCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACaa                                               <  1:592330/140‑1 (MQ=255)                                                                              aGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTc                                            >  1:1123655/1‑140 (MQ=255)                                                                              aGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTc                                            >  1:308002/1‑140 (MQ=255)                                                                               ggCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCg                                           >  1:160089/1‑140 (MQ=255)                                                                                gCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCgg                                          >  1:390291/1‑140 (MQ=255)                                                                                 ccATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGt                                         <  2:390291/140‑1 (MQ=255)                                                                                  cATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTc                                        <  2:160089/140‑1 (MQ=255)                                                                                    tttAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAg                                      >  1:632250/1‑140 (MQ=255)                                                                                     ttggTTTTTCCCGCGCATATTCAAGCAAGGAGCTGGAGCGAGAA‑GAACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGt                                     <  2:782513/137‑1 (MQ=255)                                                                                     ttAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTCCCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGt                                     <  2:525936/140‑1 (MQ=255)                                                                                     ttAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGt                                     >  2:505756/1‑140 (MQ=255)                                                                                     ttAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGt                                     <  1:505756/140‑1 (MQ=255)                                                                                      tAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGTc                                    >  1:782513/1‑140 (MQ=255)                                                                                             tCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGTCTGTCGga                             <  2:1155472/140‑1 (MQ=255)                                                                                              cccGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGTCTGTCGgag                            >  2:728989/1‑140 (MQ=255)                                                                                                   gcATATTCCAGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGTCTGTCGGAGAGACa                       >  1:706093/1‑140 (MQ=255)                                                                                                            aGCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGTCTGTCGGAGAGACAAGAAAATTc              <  2:706093/140‑1 (MQ=255)                                                                                                             gCAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGTCTGTCGGAGAGACAAGAAAATTcc             >  2:953313/1‑140 (MQ=255)                                                                                                              cAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGTCTGTCGGAGAGACAAGAAAATTCCa            <  2:48310/140‑1 (MQ=255)                                                                                                              cAAGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGTCTGTCGGAGAGACAAGAAAATTCCa            >  1:140846/1‑140 (MQ=255)                                                                                                               aaGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGTCTGTCGTAGAGACAAGAAAATTCCaa           >  1:299140/1‑140 (MQ=255)                                                                                                               aaGGAGCTGGAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGTCTGTCGGAGAGACAAGAAAATTCCaa           >  1:1215825/1‑140 (MQ=255)                                                                                                                       ggAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGTCTGTCGGAGAGACAAGAAAATTCCAAATATAAAt   <  2:1099011/140‑1 (MQ=255)                                                                                                                        gAGCGAGAAGG‑ACGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGTCTGTCGGAGAGACAAGAAAATTCCAAATATAAAtt  >  1:689469/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                CCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACATTGATAACAATTCGGTCAGTCTGTCGGAGAGACAAGAAAATTCCAAATATAAATT  >  NC_000913/997347‑997606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑