BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	224,933	A→G	noncoding (1163/1542 nt)	rrsH →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	224,933	0	A	G	91.3%	77.4 / 49.4	298	noncoding (1163/1542 nt)	rrsH	16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base A (14/12); new base G (107/165); total (121/177)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 305 total aligned reads displayed.

GTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATA  >  NC_000913/224794‑225018

gTGCCTTCGGGAACTGCGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCa                                                                                       >  2:1492743/1‑140 (MQ=21)

   ccTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTg                                                                                    <  1:1757734/140‑1 (MQ=34)

      tCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATa                                                                                 <  2:1737158/140‑1 (MQ=34)

              ttcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCggag                                                                         >  1:1142337/5‑140 (MQ=2)

              ttcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCggag                                                                         <  1:564328/136‑1 (MQ=2)

               tcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCggagg                                                                        <  1:835615/137‑1 (MQ=2)

               tcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCggagg                                                                        <  2:341020/137‑1 (MQ=2)

                tgAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTggagga                                                                       <  1:1704560/140‑1 (MQ=32)

                      aGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTg                                                                  <  1:586664/140‑1 (MQ=2)

                        gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACGAACCGGAGGAAGGTggg                                                                >  1:417530/5‑140 (MQ=2)

                        gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                                                               >  2:951339/5‑140 (MQ=2)

                        gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                                                               >  1:1211099/5‑140 (MQ=2)

                        gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCggagg                                                                        >  2:87623/5‑131 (MQ=2)

                        gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                                                               >  1:1184577/5‑140 (MQ=2)

                        gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                                                               >  1:24334/5‑140 (MQ=2)

                        gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                                                               >  2:765329/1‑140 (MQ=2)

                        gTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTgggg                                                               >  1:1488588/1‑140 (MQ=32)

                         tggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGa                                                              <  2:934162/137‑1 (MQ=2)

                          ggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGAt                                                             <  1:110111/138‑1 (MQ=2)

                          gctgcATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGAt                                                             >  2:1540853/1‑140 (MQ=32)

                           gtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATg                                                            >  2:563433/2‑140 (MQ=2)

                                  gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                     <  2:129289/138‑1 (MQ=2)

                                  gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                     <  1:87241/138‑1 (MQ=11)

                                  gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                     >  1:705732/3‑140 (MQ=11)

                                   ttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaaa                                                    >  1:283336/2‑139 (MQ=11)

                                    tGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaaa                                                    <  1:647495/140‑2 (MQ=2)

                                      tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtca                                                 >  2:422996/1‑140 (MQ=2)

                                      tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtca                                                 >  2:806782/1‑140 (MQ=2)

                                      tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGa                                                              >  2:477411/1‑127 (MQ=2)

                                      tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGa                                                              <  1:477411/127‑1 (MQ=2)

                                      tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtca                                                 >  1:1231169/1‑140 (MQ=2)

                                      tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtca                                                 >  1:1166517/1‑140 (MQ=2)

                                       cgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtcat                                                <  2:676606/140‑1 (MQ=2)

                                       cgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCAGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtcat                                                <  1:669422/140‑1 (MQ=2)

                                        gtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAatcatc                                               >  2:645745/1‑140 (MQ=2)

                                         tcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtcatca                                              <  1:435123/140‑1 (MQ=2)

                                         tcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtcatca                                              <  1:1012359/140‑1 (MQ=2)

                                           aGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGTGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg                                            >  1:520935/1‑140 (MQ=2)

                                           aGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg                                            <  1:1219998/140‑1 (MQ=2)

                                           aGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg                                            >  1:759649/1‑140 (MQ=11)

                                            gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           <  1:965618/140‑2 (MQ=2)

                                            gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           >  2:1036868/1‑139 (MQ=2)

                                            gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           <  2:178076/140‑2 (MQ=2)

                                            gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           >  2:1131151/1‑139 (MQ=2)

                                             cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           >  1:86000/1‑139 (MQ=2)

                                             cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           <  2:1114696/140‑2 (MQ=2)

                                             cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgcc                                          >  2:282981/1‑138 (MQ=2)

                                             cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgcc                                          >  1:458/1‑138 (MQ=2)

                                             cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           >  2:66488/1‑138 (MQ=2)

                                                 tgtcgtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt                                      <  2:700541/140‑1 (MQ=2)

                                                 tgtcgtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt                                      <  2:1149083/140‑1 (MQ=2)

                                                 tgtcgtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt                                      <  2:1677397/140‑1 (MQ=2)

                                                     gtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGa                                  >  1:977036/1‑140 (MQ=2)

                                                     gtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaaat                                                   >  2:257526/1‑121 (MQ=2)

                                                      tgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTGTTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAc                                 <  2:1173602/140‑1 (MQ=2)

                                                      tgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAc                                 <  1:1201321/140‑1 (MQ=2)

                                                      tgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAc                                 <  1:1036868/140‑1 (MQ=2)

                                                      tgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAc                                 <  2:737499/140‑1 (MQ=2)

                                                      tgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAc                                 <  2:574118/140‑1 (MQ=2)

                                                      tgAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGAc                                 <  1:1271751/140‑1 (MQ=32)

                                                       gagaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAc                                 <  2:710837/137‑1 (MQ=2)

                                                        agaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcct                               <  2:458/138‑2 (MQ=2)

                                                         gaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcctg                              <  2:533615/139‑3 (MQ=2)

                                                            gTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGc                           <  2:228743/140‑1 (MQ=2)

                                                             ttGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                >  2:1007985/1‑134 (MQ=2)

                                                              tGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTa                         <  2:1193238/140‑1 (MQ=2)

                                                              cgggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTa                         <  2:607671/139‑1 (MQ=2)

                                                               gggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                        <  1:196907/140‑1 (MQ=2)

                                                               gggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                        <  1:514722/140‑1 (MQ=2)

                                                               gggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                        <  2:1231169/140‑1 (MQ=2)

                                                               gggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                        <  1:914195/140‑1 (MQ=2)

                                                                ggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                        <  2:832369/140‑1 (MQ=2)

                                                                 gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                      <  1:788965/140‑1 (MQ=2)

                                                                 gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                      <  1:330673/140‑1 (MQ=2)

                                                                  ttAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacaca                     <  2:778466/140‑1 (MQ=2)

                                                                  ttAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacaca                     <  2:515424/140‑1 (MQ=2)

                                                                    aaGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                <  1:139174/127‑1 (MQ=2)

                                                                     aGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGt                  >  2:1771625/1‑140 (MQ=32)

                                                                     aGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGc                           <  1:626431/131‑1 (MQ=2)

                                                                     aGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGc                           >  2:626431/1‑131 (MQ=2)

                                                                     aGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGt                  >  1:358268/1‑140 (MQ=2)

                                                                      gTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGta                 <  1:156688/140‑2 (MQ=2)

                                                                      gTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGta                 <  2:910073/140‑2 (MQ=2)

                                                                      gTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGta                 <  1:828308/140‑2 (MQ=2)

                                                                      gTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGta                 <  1:729707/140‑2 (MQ=2)

                                                                       tCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGta                 >  2:374426/1‑139 (MQ=2)

                                                                       tCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAc                                 <  1:1076668/123‑1 (MQ=2)

                                                                          cGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                      <  1:1001282/131‑1 (MQ=2)

                                                                          cGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGtactac             >  2:106839/1‑140 (MQ=2)

                                                                           gCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACa            <  1:1540853/140‑1 (MQ=32)

                                                                           gCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACa            <  2:1211099/140‑1 (MQ=2)

                                                                           gCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACa            <  2:1078955/140‑1 (MQ=2)

                                                                                gAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgc       <  2:869305/140‑1 (MQ=2)

                                                                                gAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgc       <  2:977036/140‑1 (MQ=2)

                                                                                gAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgc       <  2:466811/140‑1 (MQ=2)

                                                                                gAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgc       <  2:359469/140‑1 (MQ=2)

                                                                                gAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgc       <  1:1131151/140‑1 (MQ=2)

                                                                                 aGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                        <  2:401745/122‑1 (MQ=2)

                                                                                 aGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                        >  1:401745/1‑122 (MQ=2)

                                                                                     cAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATa  <  2:1410256/140‑1 (MQ=32)

GTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATA  >  NC_000913/224794‑225018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`