BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	224,943	T→C	noncoding (1173/1542 nt)	rrsH →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	224,943	0	T	C	92.8%	94.0 / 37.4	292	noncoding (1173/1542 nt)	rrsH	16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base T (12/9); new base C (106/165); total (118/174)
Fisher's exact test for biased strand distribution p-value = 1.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 297 total aligned reads displayed.

TGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATA  >  NC_000913/224808‑225018

ttcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGACACTCTAAAGAGACTGCCGGTGACAAACCggag                                                                         <  1:1225892/136‑1 (MQ=2)

 tcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCggagg                                                                        <  2:496757/137‑1 (MQ=2)

 tcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCggagg                                                                        <  1:835615/137‑1 (MQ=2)

 tcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCggagg                                                                        >  2:944776/4‑140 (MQ=2)

 gtgAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTggagg                                                                        <  2:1778473/140‑1 (MQ=32)

  caagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCggagga                                                                       <  1:226366/138‑1 (MQ=2)

       cAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTg                                                                  <  2:816298/140‑1 (MQ=2)

          gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCggagga                                                                       >  1:631186/5‑132 (MQ=2)

          gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                                                               >  1:4573/5‑140 (MQ=2)

          gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTCGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                                                               >  1:607671/5‑140 (MQ=2)

          gTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTgggg                                                               >  1:1488588/1‑140 (MQ=32)

           tggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCGGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGa                                                              >  2:368521/4‑140 (MQ=2)

            ggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGAt                                                             <  2:271985/138‑1 (MQ=2)

            gctgcATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGAt                                                             >  2:1540853/1‑140 (MQ=32)

             gtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGAt                                                             <  1:516475/139‑1 (MQ=2)

             gtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATg                                                            >  2:563433/2‑140 (MQ=2)

             gtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATg                                                            >  2:977721/2‑140 (MQ=2)

               gcATGGTTGTCGTCAGCTCGTGTCGTAAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGAc                                                          <  2:219871/140‑1 (MQ=2)

                cATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACg                                                         >  1:1139286/1‑140 (MQ=2)

                    gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                     <  2:129289/138‑1 (MQ=2)

                    gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                     >  2:537028/3‑140 (MQ=11)

                    gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                     <  1:87241/138‑1 (MQ=11)

                        tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGa                                                              <  2:850552/128‑1 (MQ=2)

                        tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGAt                                                             <  2:882281/128‑1 (MQ=2)

                        tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtca                                                 >  2:74738/1‑140 (MQ=11)

                          gtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAatcatc                                               >  2:645745/1‑140 (MQ=2)

                             aGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATcatcat                                             >  1:583785/1‑140 (MQ=2)

                              gcgcGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg                                            <  2:701582/137‑1 (MQ=2)

                              gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           >  2:1036868/1‑139 (MQ=2)

                              gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           <  1:965618/140‑2 (MQ=2)

                              gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           <  1:1217726/140‑2 (MQ=2)

                              gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           <  1:676709/140‑2 (MQ=2)

                              gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           <  2:404608/140‑2 (MQ=2)

                              gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           >  2:1131151/1‑139 (MQ=2)

                               cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           <  2:1114696/140‑2 (MQ=2)

                               cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           <  1:733351/140‑2 (MQ=2)

                               cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           >  1:86000/1‑139 (MQ=2)

                               cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           <  1:1175860/140‑2 (MQ=2)

                               cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgcc                                          >  1:1097507/1‑138 (MQ=2)

                               cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgcc                                          >  2:963887/1‑138 (MQ=2)

                               cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgcc                                          >  1:16965/1‑138 (MQ=2)

                               cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           <  2:78243/139‑2 (MQ=2)

                               cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           <  1:66488/139‑2 (MQ=2)

                               cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           >  2:66488/1‑138 (MQ=2)

                                  gtgtCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCt                                       >  1:386029/1‑140 (MQ=2)

                                   tttcgtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtcatca                                              >  1:1129335/5‑132 (MQ=2)

                                   tgtcgtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt                                      >  2:462433/1‑140 (MQ=2)

                                   tgtcgtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt                                      <  2:1149083/140‑1 (MQ=2)

                                   tgtcgtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt                                      <  1:476361/140‑1 (MQ=2)

                                   tgtcgtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt                                      <  2:1677397/140‑1 (MQ=2)

                                   tgtcgtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt                                      <  1:537028/140‑1 (MQ=2)

                                    gtcgtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt                                      >  1:1206921/4‑140 (MQ=2)

                                    gtcgtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTa                                     >  1:119785/4‑140 (MQ=2)

                                       gtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGa                                  >  1:977036/1‑140 (MQ=2)

                                        tgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTGTTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAc                                 <  2:1173602/140‑1 (MQ=2)

                                        tgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAc                                 <  1:262628/140‑1 (MQ=2)

                                        tgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAc                                 <  2:574118/140‑1 (MQ=2)

                                        tgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAc                                 <  2:910628/140‑1 (MQ=2)

                                         gagaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAc                                 <  2:765722/137‑1 (MQ=2)

                                         gagaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                           <  1:1024654/126‑2 (MQ=2)

                                         gagaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                <  1:765329/137‑1 (MQ=2)

                                         gagaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                <  1:15439/137‑1 (MQ=2)

                                          agaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGCCCCTTATGAcct                               <  1:410015/138‑2 (MQ=2)

                                           gaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcctg                              <  2:685445/139‑3 (MQ=2)

                                              gTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGc                           <  2:386029/140‑1 (MQ=2)

                                               ttGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                <  1:1007985/134‑1 (MQ=2)

                                               ttGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                >  2:1007985/1‑134 (MQ=2)

                                                tGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTa                         <  2:1193238/140‑1 (MQ=2)

                                                cgggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTa                         <  2:607671/139‑1 (MQ=2)

                                                 gggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGTGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                        <  1:321697/140‑1 (MQ=2)

                                                 gggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                        <  2:233155/140‑1 (MQ=2)

                                                 gggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                        <  2:1231169/140‑1 (MQ=2)

                                                 gggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                        <  2:721512/140‑1 (MQ=2)

                                                 gggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                        <  1:914195/140‑1 (MQ=2)

                                                   gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCTGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                      <  1:502462/140‑1 (MQ=2)

                                                   gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                      <  2:14476/140‑1 (MQ=2)

                                                   gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                      <  1:788965/140‑1 (MQ=2)

                                                   gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                      <  2:519490/140‑1 (MQ=2)

                                                   gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                      <  2:177081/140‑1 (MQ=2)

                                                    ttAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTacaca                     >  1:1248413/1‑140 (MQ=33)

                                                    ttAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCGAATCATCATGCCCCTTATGACCTGGGCTacaca                     >  1:430534/1‑140 (MQ=2)

                                                      aaGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                >  2:139174/1‑127 (MQ=2)

                                                      aaGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                <  1:139174/127‑1 (MQ=2)

                                                      aaGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACg                   <  2:468821/140‑1 (MQ=2)

                                                      aaGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACg                   >  1:196622/1‑140 (MQ=2)

                                                       aGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                        >  1:356619/1‑135 (MQ=2)

                                                       aGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGc                           >  2:626431/1‑131 (MQ=2)

                                                        gTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGta                 <  1:828308/140‑2 (MQ=2)

                                                           ccGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTa              >  1:1150884/1‑140 (MQ=2)

                                                            cGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                      <  1:1001282/131‑1 (MQ=2)

                                                            cGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGtactac             >  2:106839/1‑140 (MQ=2)

                                                             gCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACa            <  2:1211099/140‑1 (MQ=2)

                                                             gCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACa            <  2:1078955/140‑1 (MQ=2)

                                                             gCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACa            <  1:71439/140‑1 (MQ=2)

                                                             gCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACa            <  2:342908/140‑1 (MQ=2)

                                                              cAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACa            <  1:1067416/140‑1 (MQ=2)

                                                                  gAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgc       <  2:870557/140‑1 (MQ=2)

                                                                   aGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgc       <  2:294938/140‑1 (MQ=2)

                                                                       cAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATa  >  1:1739724/1‑140 (MQ=33)

                                                                           ccctATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgc       <  2:427183/128‑1 (MQ=2)

TGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATA  >  NC_000913/224808‑225018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`