Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,863,500	A→G	T244A (ACC→GCC)	gapA →	glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,863,500	0	A	G	100.0%	129.4 / NA	36	T244A (ACC→GCC)	gapA	glyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/15);  total (21/15)

CGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGAAGTTT  >  NC_000913/1863364‑1863635                                                                                                                                         |                                                                                                                                        cggcgCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCg                                                                                                                                      <  1:1317123/140‑1 (MQ=255)                catcatCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAg                                                                                                                       >  2:1289128/1‑140 (MQ=255)                   catcCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTg                                                                                                                    >  2:1491045/1‑140 (MQ=255)                   catcCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTg                                                                                                                    >  2:1565159/1‑140 (MQ=255)                    atcCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGc                                                                                                                   <  2:1534248/140‑1 (MQ=255)                           ccTCTACCGGTGCTGCT‑AAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACg                                                                                                           >  1:1784913/1‑140 (MQ=255)                              ctACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAg                                                                                                         <  1:1681724/140‑1 (MQ=255)                                       ctgctAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCaaa                                                                                                >  1:1585733/1‑140 (MQ=255)                                                  gTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTaa                                                                                     <  1:1610873/140‑1 (MQ=255)                                                   tAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTaaa                                                                                    >  1:1418290/1‑140 (MQ=255)                                                    aGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAg                                                                                   >  1:1630416/1‑140 (MQ=255)                                                          aGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAgctgctg                                                                             >  1:1740036/1‑140 (MQ=255)                                                              cTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTCTCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGa                                                                         <  2:1410428/140‑1 (MQ=255)                                                               tGCCAGAACTGAATGGCAAACCGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGaa                                                                        >  2:1302530/1‑140 (MQ=255)                                                                gCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAg                                                                       <  1:1651936/140‑1 (MQ=255)                                                                     aaCTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGaa                                                                  >  1:1290998/1‑140 (MQ=255)                                                                      aCTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGaaa                                                                 >  1:1341144/1‑140 (MQ=255)                                                                       cTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAAt                                                                >  2:1670767/1‑140 (MQ=255)                                                                            tGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAg                                                           <  1:1491045/140‑1 (MQ=255)                                                                               cAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCg                                                        >  2:1373020/1‑140 (MQ=255)                                                                                 aaCTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGtt                                                      <  2:1521809/140‑1 (MQ=255)                                                                                    tgactgGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTg                                                   >  1:1240902/1‑140 (MQ=255)                                                                                       ctgGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGc                                                <  2:1259100/140‑1 (MQ=255)                                                                                        tgGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCt                                               <  1:1565159/140‑1 (MQ=255)                                                                                               gcgttccgcgttccGACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGa                                        <  1:1283207/140‑1 (MQ=255)                                                                                                cgttccgcgttccgACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCCGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTTAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGaa                                       >  2:1752544/1‑140 (MQ=255)                                                                                                  ttccgcgttccgACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGa                                     <  2:1281229/140‑1 (MQ=255)                                                                                                   tccgcgttccgACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGAt                                    <  2:1585733/140‑1 (MQ=255)                                                                                                    ccgcgttccgACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATg                                   >  1:1301095/1‑140 (MQ=255)                                                                                                        gttccgACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACgt                               <  2:1784913/140‑1 (MQ=255)                                                                                                        gttccgACCCCGAACGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCAAAGATGACgt                               <  1:1289128/140‑1 (MQ=255)                                                                                                                    aaCGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCgt                   >  1:1771668/1‑139 (MQ=255)                                                                                                                    aaCGTATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGa                   >  2:1641395/1‑140 (MQ=255)                                                                                                                        tATCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTc               >  2:1711345/1‑140 (MQ=255)                                                                                                                         aTCTGTAGTTGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCa              >  1:1559814/1‑140 (MQ=255)                                                                                                                                 ttGACCTGGCCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGaa      >  1:1660790/1‑140 (MQ=255)                                                                                                                                   gacctggccGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGAAGt    >  2:1243743/1‑140 (MQ=255)                                                                                                                                   gacctggccGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGAAGt    >  1:1780324/1‑140 (MQ=255)                                                                                                                                   gacctggccGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGAAGt    >  2:1562909/1‑140 (MQ=255)                                                                                                                                     cctggccGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGAAGttt  <  2:1341144/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGAAGTTT  >  NC_000913/1863364‑1863635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑