Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,616,162	C→A	A23A (GCC→GCA)	bepA →	beta‑barrel assembly‑enhancing protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,616,162	0	C	A	100.0%	69.2 / NA	22	A23A (GCC→GCA)	bepA	beta‑barrel assembly‑enhancing protease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/9);  total (13/9)

CGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCCCCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTACGCGGCAGCG  >  NC_000913/2616025‑2616283                                                                                                                                          |                                                                                                                          cggcgCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGcacc                                                                                                                         >  1:1461111/1‑140 (MQ=255)     gcgGTGGGAACGATATTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCg                                                                                                                     <  1:1507287/140‑1 (MQ=255)             aaCGATATTTCACAGTATCGGTGAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGa                                                                                                             <  1:1653919/140‑1 (MQ=255)               cGATATTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACa                                                                                                           <  2:1783508/140‑1 (MQ=255)                 atatTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAgc                                                                                                         >  2:1608756/1‑140 (MQ=255)                              tCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGc                                                                                            <  2:1461111/140‑1 (MQ=255)                               cGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGcc                                                                                           >  1:1585627/1‑140 (MQ=255)                                 gTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCgg                                                                                         >  2:1767937/1‑140 (MQ=255)                                            aTCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAAcc                                                                              >  1:1430988/1‑140 (MQ=255)                                                         cAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCa                                                                 <  1:1608756/140‑1 (MQ=255)                                                           ggATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACg                                                               >  1:1264094/1‑140 (MQ=255)                                                              tAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCtt                                                            >  2:1268661/1‑140 (MQ=255)                                                                   gTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCAt                                                       <  1:1767937/140‑1 (MQ=255)                                                                      aTGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTgg                                                    >  1:1729938/1‑140 (MQ=255)                                                                      aTGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTgg                                                    >  2:1622303/1‑140 (MQ=255)                                                                           cAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAgg                                               <  2:1264094/140‑1 (MQ=255)                                                                             ggCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGaa                                             >  1:1479011/1‑140 (MQ=255)                                                                                   tGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAg                                       >  2:1614954/1‑140 (MQ=255)                                                                                        aaaaaCCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATggg                                  <  2:1585627/140‑1 (MQ=255)                                                                                                             aTTGCTGCTATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCt             >  1:1377525/1‑140 (MQ=255)                                                                                                                  tgctATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTAcgcg        >  1:1277572/1‑140 (MQ=255)                                                                                                                   gctATGACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTACGCgg       >  2:1440698/1‑140 (MQ=255)                                                                                                                        gACCATTGGTCAGGTAGCACCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTACGCGGCAgcg  <  2:1479011/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                          CGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCCCCGGCGTTTGCCGACAGCGCAGACACCTTGCCGGATATGGGAACCTCCGCAGGAAGCACGCTTTCCATTGGTCAGGAAATGCAGATGGGCGACTATTATGTCCGCCAGCTACGCGGCAGCG  >  NC_000913/2616025‑2616283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑