Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,886,861	T→C	Y120C (TAT→TGT)	cas3 ←	CRISPR‑associated endonuclease/helicase Cas3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,886,861	0	T	C	100.0%	130.4 / NA	36	Y120C (TAT→TGT)	cas3	CRISPR‑associated endonuclease/helicase Cas3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/18);  total (18/18)

TAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCAAACTTTC  >  NC_000913/2886728‑2886990                                                                                                                                      |                                                                                                                                  tAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGAc                                                                                                                             <  2:1304100/140‑1 (MQ=255)       aaaaCCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTg                                                                                                                       <  2:1433799/140‑1 (MQ=255)              tgatgTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATg                                                                                                                >  2:1402940/1‑140 (MQ=255)              tgatgTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATg                                                                                                                >  1:1379374/1‑140 (MQ=255)              tgatgTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATg                                                                                                                >  1:1707800/1‑140 (MQ=255)                     cTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAAt                                                                                                         >  1:1524335/1‑140 (MQ=255)                          aCGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTTAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCCCCATGATTAAATTTACg                                                                                                    <  1:1615323/140‑1 (MQ=255)                           cGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACgg                                                                                                   <  2:1431566/140‑1 (MQ=255)                             gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGca                                                                                                 >  1:1477912/1‑140 (MQ=255)                               ctctACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGcaca                                                                                               >  1:1772086/1‑140 (MQ=255)                                  tACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACAttt                                                                                            >  1:1463148/1‑140 (MQ=255)                                   ccccATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTTAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTtg                                                                                           <  2:1650390/139‑1 (MQ=255)                                   aCCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTtg                                                                                           <  1:1674102/140‑1 (MQ=255)                                        tGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTg                                                                                      >  2:1566714/1‑140 (MQ=255)                                          gAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGAt                                                                                    <  1:1668656/140‑1 (MQ=255)                                                       aTAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTg                                                                       >  1:1282650/1‑140 (MQ=255)                                                            gATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGc                                                                  >  1:1753479/1‑140 (MQ=255)                                                             aTGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCg                                                                 >  1:1333366/1‑140 (MQ=255)                                                               gaggagCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGtt                                                               >  1:1309632/1‑140 (MQ=255)                                                                aggagCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTg                                                              <  1:1513423/140‑1 (MQ=255)                                                                  gagCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCa                                                            >  1:1717354/1‑140 (MQ=255)                                                                      ggCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGAt                                                        >  1:1419533/1‑140 (MQ=255)                                                                      ggCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGAt                                                        >  2:1323981/1‑140 (MQ=255)                                                                      ggCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGAt                                                        >  2:1353088/1‑140 (MQ=255)                                                                       gCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGAtt                                                       >  1:1638038/1‑140 (MQ=255)                                                                            aaaaaaaCTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAAtt                                                  <  2:1477912/140‑1 (MQ=255)                                                                                           aTCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTctg                                   <  2:1772086/140‑1 (MQ=255)                                                                                                cGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGAt                              <  2:1354311/140‑1 (MQ=255)                                                                                                        tGCTCTGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTg                      <  2:1638038/140‑1 (MQ=255)                                                                                                           tctGAAAGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGaa                   <  2:1309632/140‑1 (MQ=255)                                                                                                                aaGTGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGta              >  2:1468416/1‑140 (MQ=255)                                                                                                                   tGAATCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTatat           <  2:1717354/140‑1 (MQ=255)                                                                                                                       tCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCaaa       <  2:1524335/140‑1 (MQ=255)                                                                                                                       tCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCaaa       <  2:1282650/140‑1 (MQ=255)                                                                                                                       tCCTGGTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACAATTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCaaa       <  1:1402940/140‑1 (MQ=255)                                                                                                                            gTTAAACCAACACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCAAACTTTc  <  2:1379374/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  TAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCCAACTTTCTGCTGATTTATATTGGAATCGTATATCAAACTTTC  >  NC_000913/2886728‑2886990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑