Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,426,967	T→C	noncoding (67/76 nt)	alaU ←	tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,426,967	0	T	C	100.0%	12.6 / NA	5	noncoding (67/76 nt)	alaU	tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/5);  total (0/5)

GATGGTGGAGCTATGCGGGATCGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACC  >  NC_000913/3426956‑3427100            |                                                                                                                                      acaaatggagccaAGCGGGATCGAACCGCTGACCTCCTGCTTGCAAGGCAGGCGCTCTCCCAGCTGAGCTATGGCCCCTTAAtgggatcaacactcatgtgttgataatggtgggccttagtggactcgaaccac            <  1:192543‑M1/130‑54 (MQ=255) acaaatggagccaAGCGGGATCGAACCGCTGACCTCCTGCTTGCAAGGCAGGCGCTCTCCCAGCTGAGCTATGGCCCCTTAAtgggatcaacactcatgtgttgataatggtgggccttagtggactcgaaccac            >  2:192543‑M1/6‑82 (MQ=255)    aatggagCCAAGCGGGATCGAACCGCTGACCTCCTGCTTGCAAGGCAGGCGCTCTCCCAGCTGAGCTATGGCCCCTTAAtgggatcaacactcatgtgttgataatggtgggccttagtggactcgaaccaccgacctca    <  1:11709‑M1/138‑62 (MQ=255)      ttgagCCAAGCGGGATCGAACCGCTGACCTCCTGCTTGCAAGGCAGGCGCTCTCCCAGCTGAGCTATGGCCCCTTAAtgggatcaacactcatgtgttgataatggtgggccttagtggactcgaaccaccgacctcacc  <  2:1116214‑M1/138‑64 (MQ=255)      tggAGCCAAGCGGGATCGAACCGCTGACCTCCTGCTTGCAAGGCAGGCGCTCTCCCAGCTGAGCTATGGCCCCTTAAtgggatcaacactcatgtgttgataatggtgggccttagtggactcgaaccaccgacctcacc  <  1:1104791‑M1/140‑64 (MQ=255)      tggAGCCAAGCGGGATCGAACCGCTGACCTCCTGCTTGCAAGGCAGGCGCTCTCCCAGCTGAGCTATGGCCCCTTAAtgggatcaacactcatgtgttgataatggtgggccttagtggactcgaaccaccgacctcacc  <  2:556350‑M1/140‑64 (MQ=255)      tggAGCCAAGCGGGATCGAACCGCTGACCTCCTGCTTGCAAGGCAGGCGCTCTCCCAGCTGAGCTATGGCCCCTTAAtgggatcaacactcatgtgttgataatggtgggccttagtggactcgaaccaccgacctcacc  <  2:771158‑M1/140‑64 (MQ=255)            |                                                                                                                                      GATGGTGGAGCTATGCGGGATCGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACC  >  NC_000913/3426956‑3427100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑