Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,835,418	T→C	N171S (AAT→AGT)	yicJ ←	putative xyloside transporter YicJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,835,418	0	T	C	100.0%	185.7 / NA	50	N171S (AAT→AGT)	yicJ	putative xyloside transporter YicJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/25);  total (25/25)

GACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGA  >  NC_000913/3835283‑3835547                                                                                                                                        |                                                                                                                                  gACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAAc                                                                                                                               >  2:1565621/1‑140 (MQ=255)        ttgtAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGc                                                                                                                        <  2:1690201/140‑1 (MQ=255)          gtAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGcat                                                                                                                      <  1:1781076/140‑1 (MQ=255)                ggtgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAg                                                                                                                >  1:1767622/1‑140 (MQ=255)                ggtgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAg                                                                                                                >  1:1350657/1‑140 (MQ=255)                  tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGaa                                                                                                              >  1:1433745/1‑140 (MQ=255)                  tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGaa                                                                                                              <  2:1721462/140‑1 (MQ=255)                  tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGaa                                                                                                              <  2:1614659/140‑1 (MQ=255)                  tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGaa                                                                                                              <  2:1587608/140‑1 (MQ=255)                  tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGaa                                                                                                              >  1:1275777/1‑140 (MQ=255)                  tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGaa                                                                                                              >  1:1536361/1‑140 (MQ=255)                  tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGaa                                                                                                              >  2:1468516/1‑140 (MQ=255)                  tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGaa                                                                                                              >  2:1385419/1‑140 (MQ=255)                  tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGaa                                                                                                              <  1:1654510/140‑1 (MQ=255)                     ttCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAg                                                                                                           >  1:1325630/1‑140 (MQ=255)                     ttCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAg                                                                                                           <  2:1332504/140‑1 (MQ=255)                            cgTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACa                                                                                                            <  2:1300235/132‑1 (MQ=255)                            cgTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACa                                                                                                            >  1:1300235/1‑132 (MQ=255)                            cgTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAg                                                                                                    >  1:1562328/1‑140 (MQ=255)                                  ttAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGcc                                                                                              >  2:1777867/1‑140 (MQ=255)                                    aGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTc                                                                                            <  1:1473399/140‑1 (MQ=255)                                           aagaaACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGt                                                                                     >  1:1323515/1‑140 (MQ=255)                                           aagaaACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGt                                                                                     >  2:1562294/1‑140 (MQ=255)                                                       gCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACaaa                                                                         >  1:1718908/1‑140 (MQ=255)                                                           gCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGc                                                                     <  1:1507107/140‑1 (MQ=255)                                                                atGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGa                                                                >  1:1603192/1‑140 (MQ=255)                                                                         accacGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCga                                                       >  1:1665925/1‑140 (MQ=255)                                                                          ccacGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCgag                                                      <  2:1767622/140‑1 (MQ=255)                                                                          ccacGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCgag                                                      >  2:1608675/1‑140 (MQ=255)                                                                           cacGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCgaga                                                     >  2:1548997/1‑140 (MQ=255)                                                                             cGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATa                                                   >  2:1762044/1‑140 (MQ=255)                                                                                    gACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAg                                            <  2:1323515/140‑1 (MQ=255)                                                                                         cgATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCggg                                       >  1:1304139/1‑140 (MQ=255)                                                                                          gATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGt                                      <  1:1451355/140‑1 (MQ=255)                                                                                            tACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGTTACGCTGAGTCGGGTCa                                    >  2:1655722/1‑140 (MQ=255)                                                                                            gaCCGCCCTGGAAACCGAGTGGTTTAGTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCGGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGGGATACGCTGAGTCGGGTCa                                    <  2:1266369/139‑1 (MQ=255)                                                                                              ccGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCAtt                                  <  2:1562328/140‑1 (MQ=255)                                                                                              ccGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCAtt                                  <  1:1562294/140‑1 (MQ=255)                                                                                                 cccTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGt                               >  2:1751215/1‑140 (MQ=255)                                                                                                   cTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGa                             <  1:1565621/140‑1 (MQ=255)                                                                                                             aGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACaccacc                   <  2:1536361/140‑1 (MQ=255)                                                                                                             aGTGGTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACaccacc                   >  2:1633307/1‑140 (MQ=255)                                                                                                                ggTTTATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCaa                <  2:1433745/140‑1 (MQ=255)                                                                                                                  tttATTATCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATg              <  1:1708162/140‑1 (MQ=255)                                                                                                                    tattatCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATgcg            <  2:1665925/140‑1 (MQ=255)                                                                                                                      ttatCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCa          <  2:1718908/140‑1 (MQ=255)                                                                                                                         tCACCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTa       <  1:1762044/140‑1 (MQ=255)                                                                                                                           aCCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGt        >  1:1526458/1‑137 (MQ=255)                                                                                                                           aCCGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGt        <  2:1526458/137‑1 (MQ=255)                                                                                                                            ccGCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAgg    >  2:1484793/1‑140 (MQ=255)                                                                                                                              gCCAATTAAACTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGa  <  2:1604995/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  GACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGA  >  NC_000913/3835283‑3835547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑