Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,847,546	C→G	G360A (GGC→GCC)	uhpC ←	inner membrane protein sensing glucose‑6‑phosphate

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,847,546	0	C	G	100.0%	127.0 / NA	35	G360A (GGC→GCC)	uhpC	inner membrane protein sensing glucose‑6‑phosphate
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (20/15);  total (20/15)

TATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTCGGTTGCCGT  >  NC_000913/3847409‑3847681                                                                                                                                          |                                                                                                                                        tATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCa                                                                                                                                       >  1:1463437/1‑140 (MQ=255)    cGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAg                                                                                                                                    >  1:1532388/1‑140 (MQ=255)                      gCCACCCAGCAGGCGCCCCCCCCAGATAAGCAAACAAGCCGACAACCCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTg                                                                                                                  <  2:1464150/140‑1 (MQ=255)                            cagcagGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGaagaag                                                                                                            <  1:1596205/140‑1 (MQ=255)                               cAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAg                                                                                                         >  2:1538281/1‑140 (MQ=255)                                aaGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAgg                                                                                                        <  1:1773434/140‑1 (MQ=255)                                    gccGCCCCCAGATAAGCAAACAAGCCGACAACCCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGc                                                                                                    <  2:1781097/138‑1 (MQ=255)                                    gACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACCCTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGc                                                                                                    <  1:1504175/140‑1 (MQ=255)                                    gACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGc                                                                                                    <  2:1301369/140‑1 (MQ=255)                                       ccccccAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTg                                                                                                 <  1:1607262/139‑1 (MQ=255)                                         ccccAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCa                                                                                               >  1:1522788/1‑140 (MQ=255)                                            cAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCa                                                                                            >  1:1280440/1‑140 (MQ=255)                                                 aaGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAg                                                                                       <  1:1474319/140‑1 (MQ=255)                                                   gCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCt                                                                                     >  2:1694197/1‑140 (MQ=255)                                                         aaGCCGACAACCCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCaaa                                                                               <  2:1532388/140‑1 (MQ=255)                                                         aaGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCaaa                                                                               >  2:1679088/1‑140 (MQ=255)                                                             cGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGc                                                                           <  2:1732897/140‑1 (MQ=255)                                                               ccAAACCCCGTCGCCGCCCCTGCCGCCTCTTGGGGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCAt                                                                         <  2:1441140/139‑1 (MQ=255)                                                               aCAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCAt                                                                         <  1:1380481/140‑1 (MQ=255)                                                                      ccGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCcac                                                                  <  1:1694197/140‑1 (MQ=255)                                                                                              tgtgGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAAtt                                          >  2:1613702/1‑140 (MQ=255)                                                                                                           tGCCGCCGCCATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGaaaa                             <  2:1463437/140‑1 (MQ=255)                                                                                                             ccgccgccATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATc                           >  1:1630152/1‑140 (MQ=255)                                                                                                               gccgccATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCaa                         >  2:1522555/1‑140 (MQ=255)                                                                                                                 cgccATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAAt                       >  2:1762332/1‑140 (MQ=255)                                                                                                                   ccATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGTCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTc                     >  2:1730925/1‑140 (MQ=255)                                                                                                                   ccATACCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTc                     >  2:1543960/1‑140 (MQ=255)                                                                                                                     atcccGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCAt                   <  2:1712648/137‑1 (MQ=255)                                                                                                                       aCCGATTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCg                 >  2:1660743/1‑140 (MQ=255)                                                                                                                           aTTAACATCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGACAATCAAATTCATCGGccc             >  2:1250259/1‑140 (MQ=255)                                                                                                                             tAACATCTGTGGGGCAAAGACCAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTc           >  1:1511508/1‑140 (MQ=255)                                                                                                                                  tCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTCGGTTg      >  2:1682016/1‑140 (MQ=255)                                                                                                                                  tCTGTGGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTCGGTTg      <  1:1538281/140‑1 (MQ=255)                                                                                                                                     gtgGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTCGGTTGCCg   >  2:1267731/1‑140 (MQ=255)                                                                                                                                     gtgGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTCGGTTGCCg   >  2:1438218/1‑140 (MQ=255)                                                                                                                                      tgGGGCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTCGGTTGCCGt  >  2:1503167/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCATCGGCCCTCGGTTGCCGT  >  NC_000913/3847409‑3847681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑