Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,279,622 T→G F382V (TTT→GTT)  ghxP → guanine/hypoxanthine transporter GhxP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,279,6220TG96.5% 100.9 / ‑3.5 29F382V (TTT→GTT) ghxPguanine/hypoxanthine transporter GhxP
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (16/12);  total (17/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-01

ATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAA  >  NC_000913/4279490‑4279750
                                                                                                                                    |                                                                                                                                
aTCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGa                                                                                                                           <  1:1700547/140‑1 (MQ=255)
       ttGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTttgtt                                                                                                                    >  2:1408413/1‑140 (MQ=255)
         ggCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGa                                                                                                                  >  2:1249384/1‑140 (MQ=255)
                      tcctGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGt                                                                                                     <  1:1244031/140‑1 (MQ=255)
                             tCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTa                                                                                              <  1:1768130/140‑1 (MQ=255)
                               tGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACg                                                                                            >  1:1536049/1‑140 (MQ=255)
                               tGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACg                                                                                            >  2:1279168/1‑140 (MQ=255)
                                gTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACgg                                                                                           <  1:1388343/140‑1 (MQ=255)
                                  tCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCt                                                                                         >  2:1297963/1‑140 (MQ=255)
                                         tcCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCa                                                                                  >  2:1307035/1‑140 (MQ=255)
                                           cGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATc                                                                                >  1:1571892/1‑140 (MQ=255)
                                              tctctTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTg                                                                             <  2:1536049/140‑1 (MQ=255)
                                                 ctTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTg                                                                          <  1:1279168/140‑1 (MQ=255)
                                                   tACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGAc                                                                        >  2:1283065/1‑140 (MQ=255)
                                                        cGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTa                                                                   >  2:1265234/1‑140 (MQ=255)
                                                          ttCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAAc                                                                 >  1:1350091/1‑140 (MQ=255)
                                                                 gTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTaa                                                          <  1:1615248/140‑1 (MQ=255)
                                                                 gTAGGCAATGGCTCCGGCGCGGTTGTACGTTGGCCTGCTGATGCTGTCCAACATGGAGAAAATCGGCTTTGGTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTaa                                                          <  2:892465/140‑1 (MQ=255)
                                                                    tGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAg                                                       >  2:1293722/1‑140 (MQ=255)
                                                                            cTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATg                                               >  2:1739997/1‑140 (MQ=255)
                                                                            cTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATg                                               >  2:1781331/1‑140 (MQ=255)
                                                                                      gATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTcg                                     <  1:1408413/140‑1 (MQ=255)
                                                                                            cGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGActc                               <  1:1283065/140‑1 (MQ=255)
                                                                                                 gCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTg                          >  1:1425975/1‑140 (MQ=255)
                                                                                                   ctgctgATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGAt                        <  1:1297963/140‑1 (MQ=255)
                                                                                                        gatgctgaGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGgtc                   <  1:1265234/140‑1 (MQ=255)
                                                                                                           gctgaGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGGTACGGCCGTTTTCATTGTGCTGACCCGTAACATCGTTACAGGCATCATGTTCGGCTTCCCGGCCCTGGTGATTGgtcgtc                >  2:1307431/1‑140 (MQ=255)
                                                                                                              gaGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTgg             <  1:1307035/140‑1 (MQ=255)
                                                                                                                gCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGtt           >  1:1575698/1‑140 (MQ=255)
                                                                                                                         cGAAAATCGACTTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGaa  >  2:1708896/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                
ATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAA  >  NC_000913/4279490‑4279750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑