Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,191	C→A	intergenic (‑121/‑122)	sdhE ← / → ygfZ	FAD assembly factor/folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,191	0	C	A	100.0%	215.7 / NA	63	intergenic (‑121/‑122)	sdhE/ygfZ	FAD assembly factor/folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/34);  total (29/34)

TTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGCTTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTAC  >  NC_000913/3041054‑3041323                                                                                                                                          |                                                                                                                                     ttgttgTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGAtt                                                                                                                                    >  2:815073/1‑140 (MQ=255) ttgttgTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGAtt                                                                                                                                    >  2:626395/1‑140 (MQ=255)              ccATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGaa                                                                                                                       >  1:38191/1‑140 (MQ=255)                    tcttcCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGcc                                                                                                                 <  1:626395/140‑1 (MQ=255)                      ttcCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTc                                                                                                               >  2:111540/1‑140 (MQ=255)                      ttcCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTc                                                                                                               >  1:418483/1‑140 (MQ=255)                       tcCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCg                                                                                                              >  1:937625/1‑140 (MQ=255)                        ccTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGc                                                                                                             <  1:55721/140‑1 (MQ=255)                          tGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAg                                                                                                           <  2:697337/140‑1 (MQ=255)                          tGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAg                                                                                                           <  2:556061/140‑1 (MQ=255)                           gTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAg                                                                                                           >  2:609972/1‑139 (MQ=255)                           gTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAg                                                                                                           <  1:609972/139‑1 (MQ=255)                            tctcACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAg                                                                                                                      >  2:468061/1‑127 (MQ=255)                            tctcACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAg                                                                                                                      <  1:468061/127‑1 (MQ=255)                            tctcACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGa                                                                                                          >  2:970514/1‑139 (MQ=255)                            tctcACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGa                                                                                                          <  1:970514/139‑1 (MQ=255)                               cACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGc                                                                                                      <  2:326406/140‑1 (MQ=255)                               cACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGc                                                                                                      >  1:518715/1‑140 (MQ=255)                               cACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGc                                                                                                      <  1:652803/140‑1 (MQ=255)                                aCGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGca                                                                                                     >  2:870416/1‑140 (MQ=255)                                          aGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGt                                                                                           <  2:939417/140‑1 (MQ=255)                                          aGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAATAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGt                                                                                           <  1:677854/140‑1 (MQ=255)                                            tACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAg                                                                                         >  1:697603/1‑140 (MQ=255)                                                cGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGaa                                                                                     <  2:418483/140‑1 (MQ=255)                                                 ggCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGaaa                                                                                    >  2:524322/1‑140 (MQ=255)                                                    tATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCa                                                                                 <  2:518715/140‑1 (MQ=255)                                                    tATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCa                                                                                 <  1:492183/140‑1 (MQ=255)                                                          aaCGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCa                                                                           <  1:623598/140‑1 (MQ=255)                                                          aaCGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCa                                                                           <  1:88259/140‑1 (MQ=255)                                                             gtgtTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTg                                                                        <  2:697603/140‑1 (MQ=255)                                                             gtgtTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTg                                                                        <  2:937625/140‑1 (MQ=255)                                                                tttttCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCt                                                                     >  1:238455/1‑140 (MQ=255)                                                                   ttCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCa                                                                           >  1:201794/1‑131 (MQ=255)                                                                   ttCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCa                                                                           <  2:201794/131‑1 (MQ=255)                                                                     cGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAg                                                                <  1:117499/140‑1 (MQ=255)                                                                       ccTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGct                                                              <  1:111540/140‑1 (MQ=255)                                                                        cttcttTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGctc                                                             >  1:722884/1‑140 (MQ=255)                                                                           cttTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCttt                                                          <  1:524322/140‑1 (MQ=255)                                                                           cttTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCttt                                                          <  1:870416/140‑1 (MQ=255)                                                                           cttTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCATCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCttt                                                          >  2:385154/1‑140 (MQ=255)                                                                                 cGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCAt                                                    >  2:157911/1‑140 (MQ=255)                                                                                 cGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCAt                                                    <  1:815073/140‑1 (MQ=255)                                                                                    gAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAg                                                 >  1:849497/1‑140 (MQ=255)                                                                                              aaaCACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTa                                                        <  2:535878/123‑1 (MQ=255)                                                                                              aaaCACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTa                                                        >  1:535878/1‑123 (MQ=255)                                                                                                 cacCATCGCGATTGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGcc                                    >  2:682087/1‑140 (MQ=255)                                                                                                  acCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCg                                   <  2:238455/140‑1 (MQ=255)                                                                                                     atcgcgatcgcgAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACg                                      >  2:210203/1‑134 (MQ=255)                                                                                                     atcgcgatcgcgAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACg                                      <  1:210203/134‑1 (MQ=255)                                                                                                     atcgcgatcgcgAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTa                                >  1:916995/1‑140 (MQ=255)                                                                                                     atcgcgatcgcgAAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTa                                >  2:393985/1‑140 (MQ=255)                                                                                                       cgcgatcgcgaAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAg                              >  2:195280/1‑140 (MQ=255)                                                                                                       cgcgatcgcgaAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAg                              >  2:380702/1‑140 (MQ=255)                                                                                                       cgcgatcgcgaAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAg                              >  2:900657/1‑140 (MQ=255)                                                                                                         cgatcgcgaAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCATCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCa                            <  1:385154/140‑1 (MQ=255)                                                                                                           atcgcgaAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAAc                          >  2:613069/1‑140 (MQ=255)                                                                                                              gcgaAATTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCa                       <  1:393985/140‑1 (MQ=255)                                                                                                                 aaaTTAATTCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGa                    <  2:38191/140‑1 (MQ=255)                                                                                                                    ttaattCACAACAATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGActc                 >  1:41738/1‑140 (MQ=255)                                                                                                                           acaacaATTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATg          <  2:41738/140‑1 (MQ=255)                                                                                                                             aacaaTTCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGc        <  1:195280/140‑1 (MQ=255)                                                                                                                                  ttCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTa   <  1:613069/140‑1 (MQ=255)                                                                                                                                   tCAATGGATTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTac  <  1:682087/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     TTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCCTTCTTTTACGGGAATATCAGTAAACACCATCGCGATCGCGAAATTAATTCACAACAATTCAATGGCTTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTAC  >  NC_000913/3041054‑3041323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑