Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,982,037	A→G	F49S (TTC→TCC)	araE ←	arabinose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,982,037	0	A	G	100.0%	281.2 / NA	72	F49S (TTC→TCC)	araE	arabinose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/32);  total (40/32)

CAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTA  >  NC_000913/2981907‑2982168                                                                                                                                   |                                                                                                                                    cAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGCAATGGACGGCAACg                                                                                                                            >  1:57569/1‑140 (MQ=255)   ggCTGTATTTACGCCCCACGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCt                                                                                                                          <  1:247907/140‑1 (MQ=255)     cTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTcc                                                                                                                        <  1:231850/140‑1 (MQ=255)        tATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGc                                                                                                                     >  2:180938/1‑140 (MQ=255)             aCGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTa                                                                                                                <  1:201228/140‑1 (MQ=255)              cGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTAc                                                                                                               >  2:699376/1‑140 (MQ=255)                 cccAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGcc                                                                                                            <  2:653968/140‑1 (MQ=255)                  ccAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCg                                                                                                           <  1:358942/140‑1 (MQ=255)                  ccAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCg                                                                                                           >  1:6593/1‑140 (MQ=255)                  ccAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACC‑‑GCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGat                                                                                                         >  1:779243/1‑140 (MQ=255)                      gCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGatat                                                                                                       <  1:203783/140‑1 (MQ=255)                            cGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGAc                                                                                                 <  2:340955/140‑1 (MQ=255)                            cGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGAc                                                                                                 <  1:515065/140‑1 (MQ=255)                             gACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCTAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACg                                                                                                              >  2:962165/1‑126 (MQ=255)                             gACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCTAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACg                                                                                                              <  1:962165/126‑1 (MQ=255)                              aCAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCa                                                                                               <  1:5850/140‑1 (MQ=255)                                aGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCaaa                                                                                             >  1:102893/1‑140 (MQ=255)                                aGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCaaa                                                                                             >  2:791714/1‑140 (MQ=255)                                    aaCCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAAc                                                                                         >  1:573888/1‑140 (MQ=255)                                       cATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAAt                                                                                      <  1:180938/140‑1 (MQ=255)                                            aaCAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAAc                                                                                         >  1:414220/1‑132 (MQ=255)                                            aaCAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAAc                                                                                         <  2:414220/132‑1 (MQ=255)                                            aaCAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGc                                                                                 >  2:111256/1‑140 (MQ=255)                                            aaCAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGc                                                                                 >  1:858906/1‑140 (MQ=255)                                              cAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGa                                                                               >  2:105772/1‑140 (MQ=255)                                                 cgcACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCg                                                                            >  2:313082/1‑140 (MQ=255)                                                 cgcACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCg                                                                            >  2:294919/1‑140 (MQ=255)                                                 cgcACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCg                                                                            >  2:546057/1‑140 (MQ=255)                                                 cgcACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCg                                                                            >  1:880783/1‑140 (MQ=255)                                                 cgcACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCg                                                                            >  2:67436/1‑140 (MQ=255)                                                 cgcACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCg                                                                            >  2:778768/1‑140 (MQ=255)                                                 cgcACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTAGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGACATCAAGACCAAATAACAATCCTGCGACCg                                                                            >  2:692098/1‑140 (MQ=255)                                                          tgctgcACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTa                                                                   <  2:6593/140‑1 (MQ=255)                                                          tgctgcACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTa                                                                   <  2:102893/140‑1 (MQ=255)                                                          tgctgcACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTa                                                                   <  1:546057/140‑1 (MQ=255)                                                           gctgcACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTAc                                                                  <  1:313082/140‑1 (MQ=255)                                                                  cGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGaaaca                                                           <  1:294919/140‑1 (MQ=255)                                                                   gAGCATCATGCTACTAACCACCCATTCCTGCAAACGACCGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCagcagc                                                                     >  2:381300/1‑129 (MQ=255)                                                                   gAGCATCATGCTACTAACCACCCATTCCTGCAAACGACCGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCagcagc                                                                     <  1:381300/129‑1 (MQ=255)                                                                    cgCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGaaacaaa                                                         <  2:779243/139‑1 (MQ=255)                                                                    aGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGaaacaaa                                                         >  1:598246/1‑140 (MQ=255)                                                                    aGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGaaacaaa                                                         <  2:13193/140‑1 (MQ=255)                                                                    aGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGaaacaa                                                          <  1:285497/139‑1 (MQ=255)                                                                    aGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGaaacaa                                                          >  2:285497/1‑139 (MQ=255)                                                                      catcatGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAaacaaaca                                                       <  1:67436/140‑1 (MQ=255)                                                                        tcatGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACata                                                     <  2:573888/140‑1 (MQ=255)                                                                                ctaACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATAcg                                             >  2:716377/1‑140 (MQ=255)                                                                                   accaccCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATAcgccg                                          >  1:660535/1‑140 (MQ=255)                                                                                   accaccCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATAcgccg                                          >  2:486861/1‑140 (MQ=255)                                                                                     caccCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCcgcg                                        >  1:413685/1‑140 (MQ=255)                                                                                        ccATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTAt                                     >  1:742425/1‑140 (MQ=255)                                                                                        ccATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTAt                                     >  2:386005/1‑140 (MQ=255)                                                                                        ccATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTAt                                     >  1:45736/1‑140 (MQ=255)                                                                                             ccTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGc                                <  1:256643/140‑1 (MQ=255)                                                                                             ccTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGc                                >  2:638265/1‑140 (MQ=255)                                                                                                gCAAACGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCaaa                             <  1:699376/140‑1 (MQ=255)                                                                                                     cGACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACg                        <  2:660535/140‑1 (MQ=255)                                                                                                      gACTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGt                       <  1:111256/140‑1 (MQ=255)                                                                                                        cTGGTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTgg                     >  1:580063/1‑140 (MQ=255)                                                                                                          ggTCAGCACAAAGTAATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAACGAACGTGGCg                   <  2:880783/140‑1 (MQ=255)                                                                                                           gTCAGCACAAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGt                  >  1:943637/1‑140 (MQ=255)                                                                                                                cacaAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAg             <  2:413685/140‑1 (MQ=255)                                                                                                                cacaAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAg             >  1:954555/1‑140 (MQ=255)                                                                                                                  caAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGt                       >  2:43495/1‑128 (MQ=255)                                                                                                                  caAAGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGt                       <  1:43495/128‑1 (MQ=255)                                                                                                                     aGTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGAt        >  1:404919/1‑140 (MQ=255)                                                                                                                      gTGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGAtt       <  2:45736/140‑1 (MQ=255)                                                                                                                       tGATCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTc      <  1:486861/140‑1 (MQ=255)                                                                                                                          tCGGTAATGGACGGCAACGCTCCGGCGGTTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGt   >  2:336395/1‑140 (MQ=255)                                                                                                                          tCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGt   >  2:638313/1‑140 (MQ=255)                                                                                                                          tCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGt   >  2:698427/1‑140 (MQ=255)                                                                                                                          tCGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGt   >  2:961875/1‑140 (MQ=255)                                                                                                                           cGGTAATGGACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTa  <  1:638265/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                    CAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCGGTAATGAACGGCAACGCTCCGGCGATTACGCCGATATCAAGACCAAATAACAATCCTGCGACCGCAGCAGCTACCGAAACAAACATATTCATACGCCGCGTATCCCGCAAAGAACGTGGCGTTAAAGCAGATTCCGTA  >  NC_000913/2981907‑2982168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑