| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 650,809 | 0 | T | G | 30.9% | 55.9 / 18.1 | 42 | T350P (ACC→CCC) | citC | citrate lyase synthetase |
| Reads supporting (aligned to +/- strand): ref base T (10/19); new base G (13/0); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGA > NC_000913/650675‑650944 | cGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCtttt > 1:1162327/1‑140 (MQ=255) tgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTTTCCTGCGGGGGGCTTTTgtc > 2:405673/1‑138 (MQ=255) tATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAg < 1:552342/140‑1 (MQ=255) ttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTTTCTTGCGGGGGGGTTTTTACGAGCTGCcgc > 2:552342/1‑140 (MQ=255) ttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCcgc < 2:1195754/140‑1 (MQ=255) cGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGc > 1:531147/1‑140 (MQ=255) atatCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCgg > 2:1197226/1‑140 (MQ=255) cTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTg < 2:193882/140‑1 (MQ=255) gCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCggggggggt > 2:1185920/1‑134 (MQ=255) cGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTTACCTGTTTCTCCTGCGGGGGGCTTTTGACGAGCTTCCGCGTCCTGGCggggggggtc > 2:614944/1‑133 (MQ=255) cGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGTGGTc > 2:819129/1‑140 (MQ=255) cGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTATTTTTTACCTGGTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTc > 2:1235240/1‑140 (MQ=255) gAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑g < 1:614944/140‑1 (MQ=255) aGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGGCCTGGCGGGGGGGCTCAAGCA‑gg > 2:337972/1‑140 (MQ=255) ggggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTc < 1:337972/140‑1 (MQ=255) gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCt < 1:980851/140‑1 (MQ=255) gATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCaa < 2:1162327/140‑1 (MQ=255) catcaCATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAg < 1:1235240/140‑1 (MQ=255) cacaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGt < 1:405673/140‑1 (MQ=255) cacaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGt < 2:531147/140‑1 (MQ=255) cacaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCCGTTTCTCCTGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGCTCCAACA‑GGGTCTGCCAAAAAATCAGCgg > 2:1177343/1‑139 (MQ=255) caTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAACA‑GGTTCTGCAAATAATGCAGCGGGa > 1:19656/1‑140 (MQ=255) caTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACA‑GGGTCTGCCAAAAAAGCAGCGTgg > 1:1234696/1‑139 (MQ=255) ccccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTg < 1:1185920/140‑1 (MQ=255) ccccAGACTCAAGGGTGCCTGCAACGGCGGGCCGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAGCA‑GGGTCTGCAAATAATACAGCGGGGCTg > 2:69325/1‑140 (MQ=255) gACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAGCA‑GGGTCTTCAAATAATGCAGCGGGGCTGCAggg > 2:991639/1‑140 (MQ=255) gACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGGGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAggg > 1:718187/1‑140 (MQ=255) aaGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCCGGCGGGGGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGCGGCTGCAGGGGcccg > 2:1065096/1‑138 (MQ=255) aGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAGCA‑GGGTCTGCAAATAATGCAGCGGGACTGCAGGGGCCCgc > 2:975708/1‑140 (MQ=255) aGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACA‑GGGTTTGCAAATAAATCAGCGTGGCTGCCGGGGCCCgc > 2:361352/1‑140 (MQ=255) aGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCA‑GGGTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgc > 1:1031513/1‑140 (MQ=255) tGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTCACCTGTTTTTCACGAGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTTCAGCA‑AGGTTTTTGAATAATGCCGCGTGTATGCAGGGGACCgcggcg > 2:1225644/1‑140 (MQ=255) gCCTGCAACGGCGGGCTGGTTTTTTTTTATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCCAGGAGGGTTTTCCAAAAATTGC‑GCGGGGCTGCCGGGGGCCGCGgcgc > 2:1262159/1‑140 (MQ=255) ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGcgcg < 1:991639/140‑1 (MQ=255) ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGcgcg < 1:975708/140‑1 (MQ=255) ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGcgcg < 1:739891/140‑1 (MQ=255) cTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGa < 1:1209888/140‑1 (MQ=255) aaCGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGc < 1:894452/140‑1 (MQ=255) cggGCTTGTTTATTTTCATTTTTTCCCTGTTTTTCCTGCGGGGGGCTTTTGCCGGGAGGCCGCGTCCTGGCGGGAGTGCTTAAGAA‑GGGTCTGCAAAAAATGCAGTGTTTCTGCACGGGCCCGGGGGGCGCTAGCCCTga > 2:480450/1‑140 (MQ=255) cggGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACA‑GGGTCTGCAAATAATGCAGCGGGGCTGCCGGGGCCCACGGCGCGAGAACCGTgg > 2:894452/1‑139 (MQ=255) cggGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACC‑GGGTCTGCAAATAATGCAGCGTGACTGCAGGGGCCAGCGGCGCGGTAGCCgggg > 2:1209888/1‑137 (MQ=255) ttATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGtttt > 1:1001039/1‑140 (MQ=255) ttCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGcc < 1:1229985/140‑1 (MQ=255) ttCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGcc < 2:159008/140‑1 (MQ=255) aTTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagc > 1:792120/1‑140 (MQ=255) ttttCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagcag < 1:69325/140‑1 (MQ=255) tCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTg > 2:83047/1‑140 (MQ=255) tttCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTAcc > 2:1137434/1‑140 (MQ=255) ggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGa > 1:925048/1‑140 (MQ=255) | CGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGA > NC_000913/650675‑650944 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |