Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,898,139	Δ1 bp	coding (118/588 nt)	cbrC →	UPF0167 family colicin E2 tolerance protein CbrC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,898,139	0	G	.	100.0%	391.3 / NA	90	coding (118/588 nt)	cbrC	UPF0167 family colicin E2 tolerance protein CbrC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (47/43);  total (47/43)

AGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTT  >  NC_000913/3898004‑3898273                                                                                                                                        |                                                                                                                                       aGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑Tttat                                                                                                                                   <  2:998104/140‑1 (MQ=255)     gACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACt                                                                                                                               >  1:889204/1‑140 (MQ=255)      aCAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTc                                                                                                                              <  2:652454/140‑1 (MQ=255)        aGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCgg                                                                                                                            <  1:438976/140‑1 (MQ=255)        aGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCgg                                                                                                                            <  1:239546/140‑1 (MQ=255)             gTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTccc                                                                                                                       <  1:74764/140‑1 (MQ=255)              tttCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCt                                                                                                                      >  2:1004468/1‑140 (MQ=255)                tCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑Tttat                                                                                                                                   <  2:189135/125‑1 (MQ=255)                tCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑Tttat                                                                                                                                   >  1:189135/1‑125 (MQ=255)                tCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCttt                                                                                                                    >  2:800932/1‑140 (MQ=255)                    tGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCgg                                                                                                                            <  1:215900/128‑1 (MQ=255)                    tGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCgg                                                                                                                            >  2:215900/1‑128 (MQ=255)                    tGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTccc                                                                                                                       <  1:448783/133‑1 (MQ=255)                    tGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTccc                                                                                                                       >  2:448783/1‑133 (MQ=255)                    tGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTAtt                                                                                                                >  1:1080611/1‑140 (MQ=255)                    tGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTAtt                                                                                                                >  2:805210/1‑140 (MQ=255)                        tCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGt                                                                                                            >  2:169309/1‑140 (MQ=255)                        tCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGt                                                                                                            >  2:500612/1‑140 (MQ=255)                           aaaTATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTtga                                                                                                         <  2:825920/140‑1 (MQ=255)                            aaTATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTAt                                                                                                                 <  2:1020577/131‑1 (MQ=255)                            aaTATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTAt                                                                                                                 >  1:1020577/1‑131 (MQ=255)                            aaTATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTtgat                                                                                                        >  2:359395/1‑140 (MQ=255)                                tCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAg                                                                                                    <  2:521899/140‑1 (MQ=255)                                  aGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAAGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGtt                                                                                                  <  1:64819/140‑1 (MQ=255)                                    gCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGa                                                                                                >  1:1013388/1‑140 (MQ=255)                                    gCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGa                                                                                                <  2:670217/140‑1 (MQ=255)                                      cGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTtga                                                                                                         >  1:253392/1‑129 (MQ=255)                                      cGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTtga                                                                                                         <  2:253392/129‑1 (MQ=255)                                        ttACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAt                                                                                            >  2:824189/1‑140 (MQ=255)                                        ttACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAt                                                                                            >  1:1044/1‑140 (MQ=255)                                        ttACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAt                                                                                            >  1:5910/1‑140 (MQ=255)                                         tACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGa                                                                                                >  1:314695/1‑135 (MQ=255)                                         tACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGa                                                                                                <  2:314695/135‑1 (MQ=255)                                         tACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAtc                                                                                           >  2:881764/1‑140 (MQ=255)                                            cccAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATctct                                                                                        <  1:1004468/140‑1 (MQ=255)                                              cAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAtctc                                                                                         >  2:898148/1‑137 (MQ=255)                                              cAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAtctc                                                                                         <  1:898148/137‑1 (MQ=255)                                              aaaTTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGt                                                                                      <  2:398659/139‑1 (MQ=255)                                                aTTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTcc                                                                                    <  1:359395/140‑1 (MQ=255)                                                      atatCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCgtggtg                                                                              <  1:500612/140‑1 (MQ=255)                                                           atcCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTg                                                                         >  1:369473/1‑140 (MQ=255)                                                           atcCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTg                                                                         >  2:901621/1‑140 (MQ=255)                                                                aaGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGAc                                                                    <  1:800932/140‑1 (MQ=255)                                                                     aCTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTc                                                               >  2:201894/1‑140 (MQ=255)                                                                     aCTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTc                                                               >  1:812865/1‑140 (MQ=255)                                                                       tGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTctg                                                             >  1:1040930/1‑140 (MQ=255)                                                                       tGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTctg                                                             >  2:702319/1‑140 (MQ=255)                                                                       tGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTctg                                                             >  2:896165/1‑140 (MQ=255)                                                                           aaCAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTctgctgc                                                         >  1:600674/1‑140 (MQ=255)                                                                            aCAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGa                                                                     >  1:921630/1‑127 (MQ=255)                                                                            aCAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGa                                                                     <  2:921630/127‑1 (MQ=255)                                                                                   cATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGt                                                 >  1:505368/1‑140 (MQ=255)                                                                                     tttGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGttt                                               >  2:871296/1‑140 (MQ=255)                                                                                       tGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGc                                             <  2:428855/140‑1 (MQ=255)                                                                                           cAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGt                                         <  1:824189/140‑1 (MQ=255)                                                                                              gATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGt                                      >  2:522538/1‑140 (MQ=255)                                                                                                tAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGttt                                    >  1:966829/1‑140 (MQ=255)                                                                                                tAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGttt                                    <  1:896165/140‑1 (MQ=255)                                                                                                  aaaCCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTc                                  <  2:5910/140‑1 (MQ=255)                                                                                                   aaCCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTc                                  <  1:831624/139‑1 (MQ=255)                                                                                                   aaCCGTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTc                                  >  2:831624/1‑139 (MQ=255)                                                                                                       gTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgat                             <  1:881764/140‑1 (MQ=255)                                                                                                       gTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgat                             <  2:1040930/140‑1 (MQ=255)                                                                                                       gTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgat                             <  2:812865/140‑1 (MQ=255)                                                                                                       gTAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgat                             <  2:600674/140‑1 (MQ=255)                                                                                                        tAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgatg                            >  2:771779/1‑140 (MQ=255)                                                                                                        tAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgatg                            >  1:633662/1‑140 (MQ=255)                                                                                                        tAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgatg                            >  2:962200/1‑140 (MQ=255)                                                                                                        tAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgatg                            >  2:1128629/1‑140 (MQ=255)                                                                                                        tAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgatg                            >  2:1181819/1‑140 (MQ=255)                                                                                                        tAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgatg                            >  2:245421/1‑140 (MQ=255)                                                                                                        tAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgatg                            >  2:517831/1‑140 (MQ=255)                                                                                                        tAGAGTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTAAAAAGTTTGCAGGTAGTTTTCAGgatg                            >  2:881236/1‑140 (MQ=255)                                                                                                          gagTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgatgat                          <  1:871296/140‑1 (MQ=255)                                                                                                            gTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGc                        <  2:714948/140‑1 (MQ=255)                                                                                                            gTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGc                        <  2:966829/140‑1 (MQ=255)                                                                                                            gTGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGc                        <  1:702319/140‑1 (MQ=255)                                                                                                             tGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGcc                       >  1:107499/1‑140 (MQ=255)                                                                                                             tGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGcc                       <  2:889204/140‑1 (MQ=255)                                                                                                             tGCGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTAAAAAGTTTGCAGGTAGTTTTCAGGATGATGcc                       <  1:881236/140‑1 (MQ=255)                                                                                                               cGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAg                     >  1:363910/1‑140 (MQ=255)                                                                                                               cGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAg                     >  1:354998/1‑140 (MQ=255)                                                                                                               cGATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAg                     >  2:1145648/1‑140 (MQ=255)                                                                                                                gATTGCTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGc                    >  2:538677/1‑140 (MQ=255)                                                                                                                     cTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGa               <  1:771779/140‑1 (MQ=255)                                                                                                                     cTGTGAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGa               <  1:245421/140‑1 (MQ=255)                                                                                                                       gtgAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAg             >  1:110397/1‑140 (MQ=255)                                                                                                                        tgAACAACAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAgg            <  2:369473/140‑1 (MQ=255)                                                                                                                          aacaacAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGgtg          <  2:73362/140‑1 (MQ=255)                                                                                                                          aacaacAGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGgtg          <  2:15441/140‑1 (MQ=255)                                                                                                                           acaacaGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGgtgt         <  1:805210/140‑1 (MQ=255)                                                                                                                               caGACGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGaa     <  1:901621/140‑1 (MQ=255)                                                                                                                                  aCGTCA‑TTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAAttt  <  2:805940/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       AGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTT  >  NC_000913/3898004‑3898273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑