Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,685,797	G→A	intergenic (‑290/‑38)	glyA ← / → hmp	serine hydroxymethyltransferase/nitric oxide dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,685,797	0	G	A	100.0%	166.8 / NA	49	intergenic (‑290/‑38)	glyA/hmp	serine hydroxymethyltransferase/nitric oxide dioxygenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/26);  total (23/26)

GGAGAATAAAGAGCTTGCAACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGTTT  >  NC_000913/2685661‑2685933                                                                                                                                         |                                                                                                                                         ggAGAATAAAGAGCTTGCAACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTaaga                                                                                                                                       <  2:265628/140‑1 (MQ=255)    gaATAAAGAGCTTGCAACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATAc                                                                                                                                    <  1:1184746/140‑1 (MQ=255)     aaTAAAGAGCTTGCAACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACa                                                                                                                                   <  2:141073/140‑1 (MQ=255)       tAAAGAGCTTGCAACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATc                                                                                                                                 >  1:325996/1‑140 (MQ=255)       tAAAGAGCTTGCAACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATc                                                                                                                                 >  1:637885/1‑140 (MQ=255)                             ggATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGaa                                                                                                           <  1:972355/140‑1 (MQ=255)                                 ttCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGAcc                                                                                                       >  1:39976/1‑140 (MQ=255)                                    ttttCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCata                                                                                                    >  2:865926/1‑140 (MQ=255)                                         aGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCtt                                                                                               <  1:865926/140‑1 (MQ=255)                                         aGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCtt                                                                                               >  1:1075022/1‑140 (MQ=255)                                         aGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCtt                                                                                               >  1:1103087/1‑140 (MQ=255)                                              tgtgATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGc                                                                                          >  1:563714/1‑140 (MQ=255)                                               gtgATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCt                                                                                         <  2:325996/140‑1 (MQ=255)                                                  aTGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCGAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCaa                                                                                      <  2:923732/140‑1 (MQ=255)                                                     cAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAAcc                                                                                   >  1:1051658/1‑140 (MQ=255)                                                       aaTTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCAt                                                                                 <  2:637885/140‑1 (MQ=255)                                                           tttCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCt                                                                             <  1:586791/140‑1 (MQ=255)                                                           tttCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCt                                                                             <  1:59451/140‑1 (MQ=255)                                                                    atcaCATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTaaaa                                                                    >  2:1037049/1‑140 (MQ=255)                                                                     tcaCATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAg                                                                   <  1:777333/140‑1 (MQ=255)                                                                         aTTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGccac                                                               <  1:892607/140‑1 (MQ=255)                                                                             tttctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTaaaa                                                                    <  1:121531/131‑1 (MQ=255)                                                                             tttctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTaaaa                                                                    >  2:121531/1‑131 (MQ=255)                                                                             tttctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTa                                                                       <  2:941322/128‑1 (MQ=255)                                                                             tttctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTa                                                                       >  1:941322/1‑128 (MQ=255)                                                                             tttctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATc                                                           >  2:855595/1‑140 (MQ=255)                                                                               tctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATccc                                                         >  2:879195/1‑140 (MQ=255)                                                                               tctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATccc                                                         >  1:303480/1‑140 (MQ=255)                                                                               tctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATccc                                                         >  2:835329/1‑140 (MQ=255)                                                                               tctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATccc                                                         >  2:633255/1‑140 (MQ=255)                                                                                  gAAAAACACCAAAGAACCATTTACATTGCAGGGCTA‑TTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTa                                                     <  2:673478/140‑1 (MQ=255)                                                                                   aaaaaCACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGccac                                                               <  2:453466/130‑1 (MQ=255)                                                                                   aaaaaCACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGccac                                                               >  1:1020068/1‑130 (MQ=255)                                                                                   aaaaaCACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGccac                                                               >  1:453466/1‑130 (MQ=255)                                                                                   aaaaaCACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGccac                                                               <  2:1020068/130‑1 (MQ=255)                                                                                              aGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAAc                                          <  1:835329/140‑1 (MQ=255)                                                                                                   cATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGc                                     >  2:373653/1‑140 (MQ=255)                                                                                                     tttACATTGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCATTTACTGGTGGAAACGGGGCCa                                   >  1:686641/1‑140 (MQ=255)                                                                                                            tGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTTACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTaa                            <  1:481087/140‑1 (MQ=255)                                                                                                            tGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTTACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTaa                            >  2:481087/1‑140 (MQ=255)                                                                                                            tGCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTaa                            <  1:1037049/140‑1 (MQ=255)                                                                                                            tGCAGGGATATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTaa                            >  2:76872/1‑140 (MQ=255)                                                                                                             gCAGGGCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAAc                           >  1:496667/1‑140 (MQ=255)                                                                                                                  gCTATTTTTTATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCATTTACTGGTGGAAACGGGGCCAAAGTTAACCGccc                      <  2:686641/140‑1 (MQ=255)                                                                                                                          ttATAAGATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTAc              <  1:76872/140‑1 (MQ=255)                                                                                                                                gATGCATTTAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGt        <  1:86622/140‑1 (MQ=255)                                                                                                                                      tttAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGttt  <  1:879195/140‑1 (MQ=255)                                                                                                                                      tttAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGttt  <  2:1199129/140‑1 (MQ=255)                                                                                                                                      tttAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGttt  <  2:1169604/140‑1 (MQ=255)                                                                                                                                      tttAAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGttt  <  1:855595/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GGAGAATAAAGAGCTTGCAACCGGAAACGGATTTCTTTTCAGGTTTGTGATGCAAATTTTTCACTTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGTTT  >  NC_000913/2685661‑2685933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑