Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,483,268	0	T	A	20.2%	195.9 / 15.3	79	L24F (TTA→TTT)	emrK	tripartite efflux pump membrane fusion protein EmrK
Reads supporting (aligned to +/- strand):  ref base T (46/17);  new base A (0/16);  total (46/33)
Fisher's exact test for biased strand distribution p-value = 5.41e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCAT  >  NC_000913/2483129‑2483403                                                                                                                                            |                                                                                                                                        tATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaat                                                                                                                                         >  1:6383/1‑140 (MQ=255)             cAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGcc                                                                                                                             >  2:110699/1‑140 (MQ=255)                tGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAAt                                                                                                                          >  2:26505/1‑140 (MQ=255)                tGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAAt                                                                                                                          >  2:638119/1‑140 (MQ=255)                tGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAAt                                                                                                                          >  2:599521/1‑140 (MQ=255)                  acacTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATaa                                                                                                                        >  1:45362/1‑140 (MQ=255)                  acacTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGACTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATaa                                                                                                                        >  2:1112485/1‑140 (MQ=255)                   cacTCCCTGAGTTTTGTGCAAAAATTGAATCTCCATCCCCCGTGAAATAGGCGTCATCTTTATTAATCATGTCTTCTAATCCCATGGACCAAAAGGCATAGCCACTTAAAAACGAAAAAAAAAAAACTACCGCCAATaaa                                                                                                                       <  2:548921/140‑1 (MQ=255)                       cccTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCCCCTGAAAACGCAAAAAATAAAACTACCGCCAATAAAGaaa                                                                                                                   <  2:194738/139‑1 (MQ=255)                       aCCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGaaa                                                                                                                   >  1:151864/1‑140 (MQ=255)                       aCCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGaaa                                                                                                                   <  1:26505/140‑1 (MQ=255)                        ccTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTTAAAACGCAATAAATAAAACTACCGCCAATAAAGaaaa                                                                                                                  >  1:172487/1‑140 (MQ=255)                        ccTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATCCCATTGACCAATAGGCATAGGCCCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGaaaa                                                                                                                  <  1:818603/140‑1 (MQ=255)                        ccTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGACATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGaaaa                                                                                                                  >  1:917494/1‑140 (MQ=255)                        ccTGAGACTTGTGCAAAAATTGGATCTGCATCCCCCGTGACATAGGCGTCACCTGTACTAATCTTTTCTCCTAATTCCATTGACAAAAAGCCAAAGGCCCCTAAAACCGAAAAAAAAAAAACTACCGCCAATAAAGaaaa                                                                                                                  <  2:594004/140‑1 (MQ=255)                         cTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAg                                                                                                                 >  1:1292538/1‑140 (MQ=255)                            agaCTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTAt                                                                                                              >  1:637342/1‑140 (MQ=255)                                ttGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttc                                                                                                          >  1:810954/1‑140 (MQ=255)                                ttGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttc                                                                                                          >  1:821053/1‑140 (MQ=255)                                 tgtgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttct                                                                                                         >  1:1374322/1‑140 (MQ=255)                                  gtgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt                                                                                                        <  1:599521/140‑1 (MQ=255)                                  gtgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt                                                                                                        >  2:1000947/1‑140 (MQ=255)                                  gtgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt                                                                                                        >  2:1093428/1‑140 (MQ=255)                                  gtgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt                                                                                                        >  2:858903/1‑140 (MQ=255)                                  gtgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt                                                                                                        >  2:1599216/1‑140 (MQ=255)                                  gtgCAAAAATTGGATCTGCATCCCCCGTGACATGGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACAAATAGGCATAGGCACCTGAAACCGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTttctt                                                                                                        <  2:151864/140‑1 (MQ=255)                                   tgaaaaaaTTGATTCTTTATTCCCCGTCCCTTAGGGTTTATCTCACCTAATAATGTCTTTAATTTCCATCCACCAGAAGTGAAAGGCCCCTAAAAGCACAAAAAATAAAGCATCCGCCAATAAAGAAAAGTATTttcttc                                                                                                       <  2:917494/135‑1 (MQ=255)                                           ttGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGaa                                                                                               <  1:936576/140‑1 (MQ=255)                                                  tGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGttttt                                                                                        >  2:1255647/1‑140 (MQ=255)                                                    cATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTa                                                                                      >  2:867123/1‑140 (MQ=255)                                                    cATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTa                                                                                      >  1:532096/1‑140 (MQ=255)                                                     aTTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAt                                                                                     <  1:680794/140‑1 (MQ=255)                                                     aTTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAt                                                                                     <  1:1333436/140‑1 (MQ=255)                                                      ttCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAtt                                                                                    >  1:20998/1‑140 (MQ=255)                                                         cccGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGa                                                                                 >  1:527488/1‑140 (MQ=255)                                                           cGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCCCCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAt                                                                               <  1:1231020/140‑1 (MQ=255)                                                           cGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATCCCATTGACCAATAGGCATAGGCACCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAt                                                                               <  2:1327981/140‑1 (MQ=255)                                                             tGACATAGGCGTCATCTGTACTAATCATTTCTTCTAATCCCATTGACCAATAGGCATAGGCACCTGAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTa                                                                             <  2:1383954/140‑1 (MQ=255)                                                               aCATGGGCGTCAGCTGTAATACTCATGTGTTTTAATCCCAGTGACCAATAGGCAGGGACCCCTGAAAACCAAAAAAAAAAAGTTGCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAAt                                                                           <  2:1597374/140‑1 (MQ=255)                                                               aCATAGGCGTCATCTGTACTAATCATTTCTTCTAATCCCATTGACAAATAGGCAAAGGCACCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAAt                                                                           <  2:6383/140‑1 (MQ=255)                                                               aCATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCCCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAAt                                                                           <  1:696255/140‑1 (MQ=255)                                                               aCATAGGCGTCATCTGTACTAATCATGTCTTCAAATCCCATTGACAAATAGGCATAGGCCCCTAAAAACAAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAAt                                                                           <  2:637342/140‑1 (MQ=255)                                                                cATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATc                                                                          <  1:204531/140‑1 (MQ=255)                                                                  aaGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTg                                                                        <  2:492242/139‑1 (MQ=255)                                                                   aGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGt                                                                       >  2:1505140/1‑140 (MQ=255)                                                                    ggCGTCATTTGTACTAATCATTTCTTCTAATTCCATTGACAAATAGGAATAGGCACCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGtt                                                                      <  2:1292538/140‑1 (MQ=255)                                                                    ggCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAAACCAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGtt                                                                      <  2:322251/140‑1 (MQ=255)                                                                        tCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCAc                                                                  >  1:721287/1‑140 (MQ=255)                                                                          aTCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTTTTTGAATTAATCTGTTCCACta                                                                >  2:983056/1‑140 (MQ=255)                                                                          aTCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACta                                                                >  1:8660/1‑140 (MQ=255)                                                                           tCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTa                                                                             >  2:1454787/1‑126 (MQ=255)                                                                           tCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTa                                                                             <  1:1454787/126‑1 (MQ=255)                                                                            cTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtatt                                                              <  1:858903/140‑1 (MQ=255)                                                                                     tCATGTCTTCAAATTCCATTCAACAATAGGCATAGCCACCTAAAAACAAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCAt                                                     <  2:835965/140‑1 (MQ=255)                                                                                       aTGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCAttt                                                   >  1:1287348/1‑140 (MQ=255)                                                                                         gTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtct                                                 >  1:1187568/1‑140 (MQ=255)                                                                                         gTCTTCTAATTCCATTGACCAACAGGCATAGGCACCTGAAAACGAAATAAATACAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtct                                                 >  2:739889/1‑140 (MQ=255)                                                                                              ctAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAg                                            >  2:537087/1‑140 (MQ=255)                                                                                                   tCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGa                                        <  1:1541925/139‑1 (MQ=255)                                                                                                   tCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGa                                        >  2:1541925/1‑139 (MQ=255)                                                                                                    ccATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGaaa                                      <  1:854017/140‑1 (MQ=255)                                                                                                    ccATTGACAAATAGGCATAGGCCCCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGaaa                                      <  2:20998/140‑1 (MQ=255)                                                                                                      aTTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAtt                                    >  1:448230/1‑140 (MQ=255)                                                                                                      aTTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAtt                                    >  1:790262/1‑140 (MQ=255)                                                                                                       tttaCCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAttt                                   >  2:1168817/4‑140 (MQ=255)                                                                                                             aaTAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaat                             <  1:1505140/140‑1 (MQ=255)                                                                                                             aaTAGGCATAGGCACCTGAAAACGCAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaat                             <  1:1031526/140‑1 (MQ=255)                                                                                                               taggcataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattg                           >  2:834249/1‑140 (MQ=255)                                                                                                               taggcataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattg                           >  1:1578463/1‑140 (MQ=255)                                                                                                               aaggcataggccCCTAAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattg                           <  2:1357269/139‑1 (MQ=255)                                                                                                                  gcataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattgaat                        >  1:739513/1‑140 (MQ=255)                                                                                                                    ataggcaCCTGAAAGCGCAAAAAATAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCCAATTTCTCATAGATGAAATTTATGAATtgaatga                      <  1:1112485/140‑1 (MQ=255)                                                                                                                    ataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatga                      >  1:393821/1‑140 (MQ=255)                                                                                                                    ataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatga                      >  1:657318/1‑140 (MQ=255)                                                                                                                    ataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatga                      >  2:1392927/1‑140 (MQ=255)                                                                                                                    ataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatga                      >  1:1244015/1‑140 (MQ=255)                                                                                                                    ataggcaCCTGAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatga                      <  1:519267/140‑1 (MQ=255)                                                                                                                    ataggcaCCAAAAAACAAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatga                      <  2:1578463/140‑1 (MQ=255)                                                                                                                     taggcaCCTGAAAACAAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatgaa                     <  1:125797/140‑1 (MQ=255)                                                                                                                      aggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaa                    >  2:294768/1‑140 (MQ=255)                                                                                                                      aggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaa                    >  1:106131/1‑140 (MQ=255)                                                                                                                      aggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaa                    >  1:1495644/1‑140 (MQ=255)                                                                                                                         caCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAaga                 >  1:508914/1‑140 (MQ=255)                                                                                                                            cTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGca              >  1:699143/1‑140 (MQ=255)                                                                                                                                    gCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttct      <  1:42227/140‑1 (MQ=255)                                                                                                                                    gCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttct      <  1:1255647/140‑1 (MQ=255)                                                                                                                                    gCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttct      <  1:867123/140‑1 (MQ=255)                                                                                                                                      aataaataAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc    <  2:532096/140‑1 (MQ=255)                                                                                                                                      aataaataAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc    <  2:739513/140‑1 (MQ=255)                                                                                                                                      aataaataAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc    <  2:657318/140‑1 (MQ=255)                                                                                                                                      aataaataAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc    <  1:537087/140‑1 (MQ=255)                                                                                                                                      aataaaaaAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc    <  1:933440/140‑1 (MQ=255)                                                                                                                                      aataaaaaAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc    <  1:1418437/140‑1 (MQ=255)                                                                                                                                      aataaaaaAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc    <  1:693884/140‑1 (MQ=255)                                                                                                                                      aaaaaaaaaaaCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc    <  1:1617454/137‑1 (MQ=255)                                                                                                                                      aaaaaaaaaaaCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc    <  1:638119/137‑1 (MQ=255)                                                                                                                                        aaaaaaaaaTAACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCat  <  2:527488/139‑1 (MQ=255)                                                                                                                                            |                                                                                                                                        TATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCAT  >  NC_000913/2483129‑2483403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑