| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,206,435 | 0 | C | T | 27.5% | 97.8 / 25.1 | 51 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (4/33); new base T (14/0); total (18/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.37e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAT > NC_000913/3206298‑3206567 | aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCa < 2:80335/140‑1 (MQ=255) aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGa > 2:1359153/1‑140 (MQ=255) aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGa > 2:939566/1‑140 (MQ=255) aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGa > 2:637005/1‑140 (MQ=255) aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGa > 2:1402890/1‑140 (MQ=255) cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACa < 2:1045942/140‑1 (MQ=255) ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATAc < 1:939566/140‑1 (MQ=255) ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATAc < 1:637005/140‑1 (MQ=255) ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATAc < 2:519088/140‑1 (MQ=255) cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACa < 1:1402890/140‑1 (MQ=255) ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTT‑TCAGACAaaaaaaaa > 1:154618/1‑140 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑c > 1:1457531/1‑124 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑c < 2:1457531/124‑1 (MQ=255) gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTgg < 2:1487917/140‑1 (MQ=255) ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGTAATTTTTCCCAGAAAAACAaaaaaagga > 2:683846/1‑137 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAg < 1:291681/140‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAg < 2:1508031/140‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAg < 1:683846/140‑1 (MQ=255) tctGGCGCAATATTCATCCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAg < 1:1347834/140‑1 (MQ=255) ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑TCCAACAAAAACAAAAAAAGGAGTTg > 2:1258916/1‑140 (MQ=255) ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑TCAGACAAATATAAAAAAAGGAggtt > 2:400629/1‑137 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑TCAGAAAAAAAAAAAAAATGGAGGTgt > 2:29337/1‑139 (MQ=255) aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCatg < 1:400629/140‑1 (MQ=255) atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑TCAGACAAATACAAAAAATGGAGTTGCCatga > 1:150514/1‑140 (MQ=255) tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCatgat < 1:781770/140‑1 (MQ=255) tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAg < 2:1342188/140‑1 (MQ=255) gacagcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CTAGACAAATACAAAAACTGGAGTTGCCATGATGAg < 1:1023268/135‑1 (MQ=255) cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGc < 2:60266/140‑1 (MQ=255) ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa < 1:142906/140‑1 (MQ=255) ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGATG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa < 2:1433390/140‑1 (MQ=255) cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaaa < 1:357315/140‑1 (MQ=255) aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑TCAGACAAAAACAAAAAATGGAGGTGCCCTGATGAGCGGAAGta > 1:1101017/1‑140 (MQ=255) ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑TCAAACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGaaaagaa > 2:1399389/1‑136 (MQ=255) ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑TCCGACAAAAAAAAAAAATGGAGGTGCCATGATGAGGGGAAGgaata > 1:423977/1‑140 (MQ=255) gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑TCAGACAAAAAAAAAAAAAGGGGGTGCCATGGTTAGGGGAAGTAATAAga > 2:226080/1‑139 (MQ=255) gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AAATTTT‑TTACACAAAAACAAAAAAATGGGGTGTCATGATGAGCGAAAAgaataaaa > 2:1074835/1‑138 (MQ=255) gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑TTAAACAAAAACAAAAAAAGGAGTTGCCATTATGAGCGAAAGaaaaaatgtc > 2:1023268/1‑136 (MQ=255) gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa < 2:79259/140‑1 (MQ=255) ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc < 1:226080/140‑1 (MQ=255) aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg < 1:685332/140‑1 (MQ=255) cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTg < 1:1359153/140‑1 (MQ=255) aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa < 1:1399389/140‑1 (MQ=255) tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GAATTTT‑TTAGACAAAAAAAAAAAAATGGGGTGGCATTTTGAGGGAAAATAATAAAAAAAAGGCAGGGAAT‑aa > 2:762994/1‑140 (MQ=255) tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑TCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAACAAAAAGCAGGGAAT‑aa > 1:1409464/1‑140 (MQ=255) tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑aa < 2:1523019/140‑1 (MQ=255) aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTg < 2:870694/140‑1 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑TCAAACAAAAAAAAAAAATGGGGGTGCCCTGATGAGCGAAAGAAAAAAACAAAAGGCAAGGAAA‑AAAATGACAGAGAt > 2:819976/1‑140 (MQ=255) ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑TCTAACAAAAAAAAAAAATAGGGGTGGCCTGGTGAGGGAAAAAAAAAAACAAAAAGCAGAGAAA‑AAAATGAAAAAAATTGTTGc > 2:346210/1‑140 (MQ=255) cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑TCAGACAAAAAAAAAAAAAGGGGTTGGCCTGATGAGGGGAAAGAATAAAAAAAAAGGAGTGAAA‑AAAATGAAAAAGAATGTTGCTAACt > 2:1448540/1‑140 (MQ=255) aaaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCa < 2:423977/139‑1 (MQ=255) caTTTTTAGTGGTAGTTTGGGGGGGTA‑AATTTTT‑CCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCt < 2:154618/140‑1 (MQ=255) ttttAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAcc < 2:1409464/140‑1 (MQ=255) aGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa < 1:1448540/140‑1 (MQ=255) ttGTGGCGGTG‑AATTTTT‑TCACACCAAAACAAAAAATGGAGTTGCCGTGATGAGAGGAAAAAAAAAACAACAAGAAACGAATAAAGTTG‑CCGAGATTGTCGCTAAATTTAACCCCCTGATTTTTACCCCAATGCCtgatg > 2:460981/1‑140 (MQ=255) tgGCGGTG‑AATTTTT‑TCAGACAAAAAAAAAAAATGGGGGTGCCATGATGAGGGAAAGAAATAAACAACAAGCAGTGAAA‑AAATTTACAGAGATTGTCGCTAACTTTACCGCCCTGATTTTTACCCCCATGCCTGGTGACg > 2:1335860/1‑140 (MQ=255) gCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg < 1:1586949/140‑1 (MQ=255) gCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg < 1:1384746/140‑1 (MQ=255) gTG‑AATTTTT‑TCAGACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGAAAGTAAAAAACAAAAAGCAGTGAAA‑AAAATGACAGAGATTGTTGCTAACTTTACCCCCCTGATTTTTACCCCAATGCCTGATGACgtgggg > 2:619451/1‑138 (MQ=255) g‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa < 1:1447384/140‑1 (MQ=255) g‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa < 1:619451/140‑1 (MQ=255) aaTTTTT‑TCAGACAAATACAAAAAATGGGGGTGGCCTGATGAGGGAAAGTAATAAACAACAGGCAGTGAAT‑AAATTGAAAGAGATTGTTGCTAACTTTACCGCCATGATTTTTTCCCCAATGCCTGATGACGTGGTGGAt > 2:1357972/1‑140 (MQ=255) | AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAT > NC_000913/3206298‑3206567 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |