Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,829,981	T→C	D262G (GAC→GGC)	astA ←	arginine N‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,829,981	0	T	C	100.0%	273.1 / NA	69	D262G (GAC→GGC)	astA	arginine N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/39);  total (30/39)

GGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGAT  >  NC_000913/1829845‑1830115                                                                                                                                         |                                                                                                                                       ggTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCcac                                                                                                                                     >  1:1401920/1‑140 (MQ=255)    aCGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACt                                                                                                                                  <  2:553427/140‑1 (MQ=255)             caccacGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCg                                                                                                                         >  1:1131705/1‑140 (MQ=255)             caccacGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCg                                                                                                                         >  1:661632/1‑140 (MQ=255)             caccacGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCg                                                                                                                         >  1:1306868/1‑140 (MQ=255)             caccacGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCg                                                                                                                         >  1:1404467/1‑140 (MQ=255)                cacGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCggtc                                                                                                                      <  1:1239548/140‑3 (MQ=255)                cacGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGcc                                                                                                                      <  2:1163795/140‑1 (MQ=255)                 acGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGccc                                                                                                                     <  2:814276/140‑1 (MQ=255)                  cgcgGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGcc                                                                                                                      >  1:33960/1‑138 (MQ=255)                  cgcgGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGcc                                                                                                                      <  2:33960/138‑1 (MQ=255)                  cgcgGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCa                                                                                                                    >  1:211410/1‑140 (MQ=255)                  cgcgGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCa                                                                                                                    >  1:757092/1‑140 (MQ=255)                  cgcgGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCa                                                                                                                    >  2:200315/1‑140 (MQ=255)                  cgcgGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCa                                                                                                                    >  1:1159730/1‑140 (MQ=255)                    cgGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCAcc                                                                                                                  >  1:1394643/1‑140 (MQ=255)                      gAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGt                                                                                                                <  2:578501/140‑1 (MQ=255)                        aaTGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCa                                                                                                              <  2:1518376/140‑1 (MQ=255)                            gTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGa                                                                                                          <  2:201065/140‑1 (MQ=255)                            gTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGa                                                                                                          <  2:726066/140‑1 (MQ=255)                             tGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGAt                                                                                                         <  1:1348938/140‑1 (MQ=255)                             tGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGAt                                                                                                         <  1:138521/140‑1 (MQ=255)                               aTAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGt                                                                                                       <  2:757092/140‑1 (MQ=255)                               aTAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGt                                                                                                       <  2:661632/140‑1 (MQ=255)                               aTAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGt                                                                                                       >  1:954123/1‑140 (MQ=255)                                 aaTTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCg                                                                                                     >  2:1374792/1‑140 (MQ=255)                                  aTTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGa                                                                                                    <  1:200315/140‑1 (MQ=255)                                  aTTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGa                                                                                                    <  1:919601/140‑1 (MQ=255)                                   ttttCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGat                                                                                                   <  2:1175840/140‑1 (MQ=255)                                   ttttCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGat                                                                                                   <  1:554209/140‑1 (MQ=255)                                      tCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGAtata                                                                                                >  2:1618218/1‑140 (MQ=255)                                      tCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGAtata                                                                                                >  1:1416698/1‑140 (MQ=255)                                         ttGGCGACCAGGCAGGCTGGGAAACCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGtt                                                                                             >  1:1102021/1‑140 (MQ=255)                                            gCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACg                                                                                          <  2:1404467/140‑1 (MQ=255)                                                       ggcTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAAc                                                                               <  2:777616/140‑1 (MQ=255)                                                        gcTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAAcc                                                                              >  1:977833/1‑140 (MQ=255)                                                          tGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACctt                                                                            >  1:753302/1‑140 (MQ=255)                                                            ggAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACcttct                                                                          <  2:842726/140‑1 (MQ=255)                                                            ggAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACcttct                                                                          <  2:192833/140‑1 (MQ=255)                                                            ggAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACcttct                                                                          <  2:1401920/140‑1 (MQ=255)                                                            ggAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACcttct                                                                          <  2:1306868/140‑1 (MQ=255)                                                            ggAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACcttct                                                                          <  1:1374792/140‑1 (MQ=255)                                                            ggAAACCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACcttct                                                                          <  2:1102021/140‑1 (MQ=255)                                                                  cGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTcc                                                                    >  2:1494337/1‑140 (MQ=255)                                                                        gcgcAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCAcc                                                              <  2:753302/140‑1 (MQ=255)                                                                            aGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACcgcgc                                                          >  2:865334/1‑140 (MQ=255)                                                                              gCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGGTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCgg                                                        >  2:1356837/1‑140 (MQ=255)                                                                               cTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCggg                                                       <  2:1237811/140‑1 (MQ=255)                                                                                   ccTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAgg                                                   >  1:139481/1‑140 (MQ=255)                                                                                   ccTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAgg                                                   >  2:828877/1‑140 (MQ=255)                                                                                   ccTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAgg                                                   >  1:918953/1‑140 (MQ=255)                                                                                        tgtcACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCgg                                              >  1:1480310/4‑140 (MQ=255)                                                                                        tGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCgg                                              >  2:1247427/1‑140 (MQ=255)                                                                                          ccACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGtt                                            >  2:654696/1‑140 (MQ=255)                                                                                           cACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGttt                                           <  2:1394643/140‑1 (MQ=255)                                                                                                  accaGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATg                                    >  2:348202/1‑140 (MQ=255)                                                                                                     aGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTAc                                 <  2:139481/140‑1 (MQ=255)                                                                                                     aGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTAc                                 <  2:918953/140‑1 (MQ=255)                                                                                                       ccGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCt                               >  1:861276/1‑140 (MQ=255)                                                                                                       ccGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCt                               <  2:954123/140‑1 (MQ=255)                                                                                                       ccGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCt                               <  1:654696/140‑1 (MQ=255)                                                                                                        cGACTTTTACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCA‑CGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGa                             >  1:489370/1‑140 (MQ=255)                                                                                                              ttACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGAt                        <  1:1494337/140‑1 (MQ=255)                                                                                                              ttACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGAt                        <  2:1416698/140‑1 (MQ=255)                                                                                                               tACGGATGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATg                       <  2:1480310/140‑1 (MQ=255)                                                                                                                     tGGCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCA‑CGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCt                <  2:489370/140‑1 (MQ=255)                                                                                                                      ggCGCGCACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCt                >  1:281782/1‑140 (MQ=255)                                                                                                                         gcgcACGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTggg             <  2:1177222/140‑1 (MQ=255)                                                                                                                             aCGCGGTCGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTc         <  1:1247427/140‑1 (MQ=255)                                                                                                                                    cGATGCCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGAt  <  2:281782/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGAT  >  NC_000913/1829845‑1830115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑